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Preprints

There are 3747 Preprints listed.

Amplexus Takeover in the Asian Common Toad, Duttaphrynus melanostictus, in Dumka, Jharkhand, India

Kulesh Bhandari

Published: 2026-10-01
Subjects: Ecology and Evolutionary Biology, Life Sciences

I report an instance of amplexus takeover in the Asian Common Toad, Duttaphrynus melanostictus, observed at Simardhuma village, Dumka District, Jharkhand, India, on 21 July 2025. Over an 18-minute sequence, a second male repeatedly interfered with an amplectant pair until the amplectant male released the female, after which the second male assumed amplexus. Although male replacement is known [...]

Expert Elicitation on demographic consequences of seabird compensatory measures

Kate Rebecca Searle, Anastasia Frantsuzova, Maria Bogdanova, et al.

Published: 2026-09-30
Subjects: Life Sciences

1. Protected seabirds present a serious constraint to offshore renewable energy expansion due to potential effects of displacement from habitat, barrier effects to movements, and collisions with turbine blades. The process of delivering compensatory measures to offset offshore wind (OW) impacts on seabirds is underway as deployments increase in scale and pace, driving cumulative impacts to become [...]

Large differences in thermal tolerance between isolated organs in rainbow trout

Leon Michael Pfeufer, Catharina Olsson, Fredrik Jutfelt

Published: 2026-09-30
Subjects: Life Sciences

Due to climate change, research on thermal performance limits in ectotherms has received much attention in the last two decades. However, the underlying physiological mechanisms and most vulnerable organs remain largely unknown. Here, we examined contractile performance in different organs from rainbow trout during an acute warming challenge, including the heart (cardiac muscle), caudal peduncle [...]

From training to implementation: building capacity in invasion science

Lorenzo Vilizzi

Published: 2026-09-30
Subjects: Life Sciences

Effective management of biological invasions requires scientifically robust decision-support tools and the capacity to apply them consistently. Risk identification is the first step of invasion risk analysis, yet comparatively little attention has been given to how such tools are transferred into operational use. This paper describes an international training programme based on the Invasiveness [...]

Toward a General Theory of Heteromorphic Reproduction

P. William Hughes

Published: 2026-09-30
Subjects: Ecology and Evolutionary Biology, Evolution, Life Sciences, Other Ecology and Evolutionary Biology, Research Methods in Life Sciences

Organisms often divide reproductive investment among outputs that differ in morphology, timing, or reproductive mechanism. In this paper I extend the minimum-risk treatment of seed heteromorphism to the more general question of organisms with ‘portfolios’ of biologically distinct reproductive outputs. The resulting framework specifies a common investment and fitness currency, calculates returns [...]

Genomic divergence between species identifies taxonomic outliers and establishes calibrated molecular clocks

Natalie Marion Allen, Mekala Sundaram, Avril Harder, et al.

Published: 2026-09-30
Subjects: Life Sciences

For decades, molecular clocks that rely on one or a few genes have been employed to quantify evolutionary rates (e.g., anagenesis) or to help date important phylogeographic events (e.g., vicariance). Genome-wide clocks remain largely unexplored, and the degree to which genome-wide divergence scales with time is still unknown in most vertebrates. Here, we expand the concept of the molecular clock [...]

Wildlife Responses to a Resident a Family: A Three-Year Camera Census on Restored Prairie–Savanna (2024–2026)

Damian Anthony Vraniak

Published: 2026-09-29
Subjects: Ecology and Evolutionary Biology, Life Sciences

A steward-conducted trail-camera census on a restored prairie–savanna complex in Washburn County, Wisconsin, labeled every frame from ten cameras at nine fixed stations for 2024, 2025 and January–June 2026 (11,820 frames; 11,135 estimated wildlife individuals; 1,027 frames of the resident family). White-tailed deer (Odocoileus virginianus) made up 67–83% of wildlife individuals in each year. [...]

Wolf–Deer and Guild Camera-trap Detections Across Wisconsin’s Statewide Wolf Range (2018–2025), Resolved by Deer Class: Buck Selection in the Hard-Antler Season, Bears and Fawns, and Class-Specific Reference Values for Displacement

Damian Anthony Vraniak

Published: 2026-09-29
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Life Sciences

A statewide analysis of wolf-enriched Snapshot Wisconsin camera data (Vraniak 2026d) reported four things: wolves following deer, a temporary rise in that following after the February 2021 wolf harvest, displacement of deer by wolves, and avoidance of people across five co-occurring species. All of it treated deer as a single class. This companion resolves the deer into antlered bucks, antlerless [...]

Reservoir, sink, or reactor? Repositioning marine sponges as candidate indicators for coastal antimicrobial resistance monitoring

Wellington Felipe Costa

Published: 2026-09-29
Subjects: Life Sciences

Antimicrobial resistance (AMR) spreads through the environment, and in coastal areas it is investigated mainly in water and sediment. These matrices capture antimicrobial resistance genes (ARGs) passing through them but may fail to retain that information over time because of the dynamics inherent in these ecosystems. Filter-feeding invertebrates are obvious candidates for the role, and bivalves [...]

Population density, cooperation, and the evolution of toothed whale societies

Richard Connor, Sam Froman Walmsley

Published: 2026-09-29
Subjects: Behavior and Ethology, Life Sciences

Odontocete societies come in two primary forms: stable matrilineally-based groups, often with bonds between groups, and labile associations among related and unrelated females in a ‘fission-fusion’ grouping pattern. There is no general theory to explain why these differing strategies evolved. Primate models of social bond evolution, based on cooperation in food competition, lack utility for [...]

Modeling Decomposition: Beyond a Well-Mixed World

Carlos A Aguilar-Trigueros, William K Cornwell, Katherine Rocci, et al.

Published: 2026-09-29
Subjects: Botany, Ecology and Evolutionary Biology, Entomology

Carbon cycle models overlook resource patchiness and networked foraging characterizing terrestrial carbon dynamics. They treat inputs as well mixed and decomposers as acting at local scales, making substrate stoichiometry, rather than spatial access, the primary constraint on decomposition. Using deadwood decomposition by cord-forming fungi and termites as examples, we illustrate how resource [...]

Common, consequential, and actively maintained: a neo-balance view of genetic variation

Mark Christopher Bitter, Paul Schmidt, Dmitri Petrov

Published: 2026-09-29
Subjects: Ecology and Evolutionary Biology, Life Sciences

A fundamental and still unfinished task of evolutionary biology is to understand the nature of genetic variation and the evolutionary forces that shape it. The traditional approach to this problem is through retrospective inference from patterns in contemporary, static DNA sequence data. The prevailing view that emerged from these studies and from theoretical research is that segregating genetic [...]

Evenness under incomplete sampling: coupled error channels, hidden compensation, and the richness-estimation bottleneck

Luca Delvecchio

Published: 2026-09-29
Subjects: Biodiversity, Ecology and Evolutionary Biology

1. Normalized evenness combines an abundance-diversity functional with richness, so incomplete sampling acts through two coupled errors. For \(E_{\alpha,\beta}=L_\beta(D_\alpha)/L_\beta(S)\), the log-error decomposes exactly as \(T_\beta=U_\beta+V_\beta\). With observed richness, \(V_\beta\) is nonnegative whenever species are missed, but total error can have either sign. 2. We generated 950,100 [...]

Quantifying the probability of evolutionary rescue

Matthew Osmond, Hildegard Uecker, Carla Alejandre, et al.

Published: 2026-09-29
Subjects: Ecology and Evolutionary Biology

While evolutionary rescue is now a well-established term, there are subtleties in precisely defining it and conceptual complications in quantifying its probability. In this Perspective, we pay special attention to three issues: 1) How do we know that a population would go extinct in the absence of evolution? 2) How do we determine the probability of rescue if persistence is possible in the [...]

Canyoning simplifies benthic macroinvertebrate communities in Mediterranean canyons: evidence from Sierra y Cañones de Guara Natural Park

Rocío López-Flores, Diego Mota-Echeandía, Mario Gaspar, et al.

Published: 2026-09-29
Subjects: Life Sciences, Terrestrial and Aquatic Ecology

1. Recreational activities in freshwater ecosystems are increasing worldwide, yet their ecological consequences remain poorly understood. Canyoning is one of the most popular nature-based sports in Mediterranean rivers, but quantitative assessments of its effects on aquatic communities are scarce, particularly in protected areas where conservation and recreation coexist. Here, we evaluated how [...]

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