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Preprints

There are 3312 Preprints listed.

Sex-reversed males are morphologically similar but have lower testosterone levels compared to sex-concordant males in the agile frog

Nadine Lehofer, Veronika Bókony, Andrea Kásler, et al.

Published: 2026-05-29
Subjects: Ecology and Evolutionary Biology

Environment-induced sex reversal – the mismatch between genetic and phenotypic sex caused by external conditions during early development – is increasingly documented in ectothermic vertebrates, yet its fitness-related consequences in natural populations remain poorly tested. To address this gap, we compared sex-reversed XX males and sex-concordant XY phenotypic males in agile frogs (Rana [...]

Developmental Plasticity in Response to Temperature Aligns with Allen’s but not Bergmann’s Rule

Rachel Bockrath, Hailey Wimberly-Lehmann, Ned A Dochtermann, et al.

Published: 2026-05-29
Subjects: Ecology and Evolutionary Biology

Increases in average global temperatures have caused researchers to revisit how species and populations are expected to respond to changing temperatures. Allen’s rule and Bergmann’s rule describe two ecogeographic patterns where species in high (cold) latitudes have shorter limbs and larger bodies, respectively, than species at low (warm) latitudes. This pattern is purported to be due to the [...]

Methodological choices influence ecological inference in passive acoustic monitoring of a Neotropical nightjar

Liliana Piatti, Daiene Louveira Hokama de Sousa, Beatriz dos Santos Oliveira, et al.

Published: 2026-05-29
Subjects: Behavior and Ethology, Biodiversity, Population Biology

Passive acoustic monitoring is increasingly used to investigate species activity and habitat use through occupancy analyses. Yet, the complex analytical workflow, from automated detector choice to confidence thresholds and statistical modeling framework, is amongst the factors that influence ecological inference, and the extent these decisions affect modelling outputs is poorly debated. Here, we [...]

Reimagining Training for the Next Generation of Ecologists

Bruno Eleres Soares, Marc W. Cadotte, Marilyn Grell-Brisk, et al.

Published: 2026-05-29
Subjects: Education, Life Sciences

Rampant environmental degradation and biodiversity loss underscore an urgent need for ecological knowledge that can directly help address social‑environmental challenges. We argue that improved Earth stewardship needs ecologists to integrate foundational ecological knowledge to contextual understanding, relational practices, and engagement with decision‑making when proposing effective solutions. [...]

The ecologist’s guide to microclimate modelling and thermal biology in R

Ilya MacLean, Lydia G Soifer, David Klinges

Published: 2026-05-28
Subjects: Life Sciences

Many ecological studies relate organismal responses to climate, but available datasets are often poor surrogates for the conditions experienced in nature. Microclimate models address this limitation by translating standard meteorological data into estimates of local temperature, humidity, and radiation at the scales relevant to organisms. Here, we provide a practical guide to mechanistic [...]

Inconsistent findings of ageing across different feather-quality indices in a wild passerine

Claire Lok Sze Tsui, Jan Komdeur, Terry Burke, et al.

Published: 2026-05-28
Subjects: Life Sciences

In most animals, individuals tend to decline in performance in later life, known as ageing. In birds, studies of ageing have traditionally concentrated on metrics of survival and reproductive success, whereas morphological traits have received comparatively little attention. Feather quality is a key morphological trait for passerines as it contributes to flight performance, camouflage and [...]

Sexual dichromatism in agile frogs: sex-reversed males closely resemble typical male coloration

Edina Nemesházi, Veronika Bókony, Ossi Nokelainen, et al.

Published: 2026-05-28
Subjects: Ecology and Evolutionary Biology

In species with sexual dichromatism, colouration can play an important role in intraspecific communication and affect breeding success. Communication by visual signals during the breeding season has been increasingly recognized to occur in anuran amphibians. However, most studies of sexual dichromatism have focused on consistent differences between males and females, and species with varying [...]

Hidden role plasticity of the reproductive caste in a morphologically differentiated termite society

Nobuaki Mizumoto, Clement Het Kaliang, Taisuke Kanao

Published: 2026-05-28
Subjects: Life Sciences, Social and Behavioral Sciences

Reproductive division of labor is the defining characteristic of eusocial insects, separating germline-like reproductives from soma-like workers. While most studies have focused on worker sterility, it is generally assumed that developing reproductives invest only in maturation, not in colony labor. Here we show that nymphs (pre-alates) in a highly structured termite society can contribute to [...]

Identifying social learning through peering: predictions and recommendations

Elisa Bandini, Elliot Howard-Spink, T Revathe, et al.

Published: 2026-05-28
Subjects: Life Sciences

Many species exhibit the capacity for social learning. However, the importance of social learning for wild individuals’ daily lives and its role in the emergence of animal culture remains to be uncovered. As observing conspecifics may provide a relatively safe and efficient means of learning, visual species may use observational learning to acquire various types of information from others. [...]

Canopy closure re-establishes ants in young tree plantations, while low soil pH limits ant diversity

Joshua Emil Spitz, Esteve Boutaud, Felix Fornoff, et al.

Published: 2026-05-28
Subjects: Entomology, Life Sciences, Terrestrial and Aquatic Ecology

1. Tree species richness is known to enhance biodiversity and ecosystem functioning, but its effects across trophic levels during forest restoration remain insufficiently understood. In reforestation on complex terrain, habitat complexity may moderate the effect of canopy closure on animal community reassembly, a relationship further shaped by the abiotic environment. 2. Ants, as key functional [...]

China must revise its regulation for managing non-native invasive species

Jingrui Sun, Phillip Joschka Haubrock, Ali Serhan Tarkan, et al.

Published: 2026-05-27
Subjects: Political Science, Terrestrial and Aquatic Ecology

Biological invasions are a leading contributor to the global biodiversity crisis, yet existing regulatory frameworks are challenged by definitions of ‘non-native’ species that are based on geopolitical rather than ecological boundaries. This perspective highlights the critical disconnect between administrative jurisdictions and biogeographic units, with focus on China’s biosecurity laws. These [...]

Navigating Spatial Trade-offs in Restoration Planning: A Multi-Objective Optimization Framework Integrating Ecological Feasibility

Matías Moreno-Faguett, Jessica Castillo, Jose Salgado Rojas, et al.

Published: 2026-05-27
Subjects: Biodiversity, Life Sciences, Natural Resources and Conservation, Physical Sciences and Mathematics, Sustainability, Terrestrial and Aquatic Ecology

Ecosystem restoration requires decision-support tools capable of balancing ecological benefits under limited resources while explicitly accounting for the long-term likelihood of restoration success. Despite its recognized importance, ecological feasibility has rarely been formulated as an optimization objective in spatial planning, typically being treated only as a constraint or biophysical [...]

The effectiveness of overwintering Eastern oysters (Crassostrea virginica) in cold-dry storage

Jannine D Chamorro, Josephine C DeMerit, Andrew R Villeneuve, et al.

Published: 2026-05-27
Subjects: Life Sciences

In the northernmost part of the Eastern oyster (Crassostrea virginica) range, oyster farmers face challenges maintaining stock through freezing winters. To avoid leaving oysters exposed to variable field conditions, many farmers overwinter oysters outside of the water in cold-dry storage (CDS). Here, we sought to add to the limited but growing empirical research examining the effectiveness of CDS [...]

From Individuals to Networks: The Role of Variation in Plant-Pollinator Communities' Responses to Global Change

James DeWitt Crall, Marilia Gaiarsa

Published: 2026-05-27
Subjects: Biology, Ecology and Evolutionary Biology, Integrative Biology, Life Sciences

1. Plant–pollinator communities are critical for biodiversity, ecosystem function, and human well-being. Yet our ability to predict divergent species responses to environmental change, the risk of abrupt collapse, or the potential for recovery in plant-pollinator systems remains limited. 2. Here, we argue that individual variation within species may play a critical but underappreciated role in [...]

Bayesian adaptive design for citizen science data collection: Exploring tensions between data and design

Max Savery, Stjin Luca

Published: 2026-05-27
Subjects: Life Sciences, Physical Sciences and Mathematics

1. Bayesian adaptive design can be applied in spatial settings where future survey locations need to be selected based on already available data. An important use case of adaptive design is the recommendation of locations for opportunistic, citizen science collection of species observation data, where some areas are already overrepresented and others are severely undersampled. 2. This work [...]

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