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Preprints

There are 3326 Preprints listed.

From social experience to social behaviour: hormonal and behavioural phenotypes during adolescence in male guinea pigs

Melanie Gleske, S. Helene Richter, Sylvia Kaiser

Published: 2026-06-02
Subjects: Animal Sciences, Biology, Life Sciences, Social and Behavioral Sciences

Adolescence is the transition from juvenility to adulthood and is characterized by prominent endocrine, neural and behavioural alterations. Thus, adolescence represents a sensitive phase during which social experiences can shape endocrine and behavioural phenotypes. Although the influence of the social environment during adolescence has been widely investigated, most studies assessed such effects [...]

Between categories and continua: growth-form organization in global leaf economics spectrum space

Stephen Antheny Mekwan

Published: 2026-06-02
Subjects: Ecology and Evolutionary Biology

The leaf economics spectrum (LES) describes a globally coordinated trade-off between acquisitive and conservative plant resource-use strategies, yet how major plant growth forms are organized within multidimensional LES space and the extent to which these patterns reflect evolutionary history remain incompletely resolved. Using species-level trait data from the TRY Plant Trait Database, we [...]

Motivations and organizational models for private sector biodiversity engagement

Matthew Selinske, Sarah Bekessy, Prue Addison, et al.

Published: 2026-06-02
Subjects: Agriculture, Ecology and Evolutionary Biology, Social and Behavioral Sciences

Private sector engagement is increasingly recognized as critical for addressing global biodiversity loss, yet the recent IPBES global assessment of business and biodiversity confirms that current economic conditions remain largely incompatible with the transformative change required. We conducted 30 semi-structured interviews with business leaders and sustainability practitioners across multiple [...]

Gradual development and chance beget individuality

Sean M. Ehlman, John McNamara, Ulrike Scherer, et al.

Published: 2026-06-02
Subjects: Behavior and Ethology, Developmental Biology, Ecology and Evolutionary Biology, Evolution

Behaviors – and thus behavioral individuality – rarely emerge fully formed but are instead built gradually through development, shaped by processes involving learning, skill formation, and experience. Prevailing theory in behavioral ecology, however, has largely focused on static equilibrium outcomes where behaviors are analyzed only as fully formed traits, often neglecting development. Here, we [...]

Concise guidelines and complementary checklists for improving research reliability and reproducibility

Jason Pither, Mathew Vis-Dunbar

Published: 2026-06-02
Subjects: Life Sciences

Checklists to address reproducibility shortfalls have proliferated but tend to be discipline- or research stage-specific and lack context or learning opportunities. We offer an alternative: one page, every stage, with a reason for each recommendation.

Evaluating the potential of molecular dietary analysis of predators for the detection of emerging plant pests

Kyle A Miller, Molly Davidson, Chris Hirst, et al.

Published: 2026-06-01
Subjects: Agriculture, Behavior and Ethology, Biodiversity, Biosecurity, Ecology and Evolutionary Biology, Entomology, Forest Biology, Forest Management, Forest Sciences, Genetics, Laboratory and Basic Science Research Life Sciences, Life Sciences, Research Methods in Life Sciences

Monitoring plant pests is crucial for maximising yields across agricultural and forest production systems, but also for the mitigation of invasive species spread. Traditional monitoring methods, such as mass trapping and direct observation, scale poorly and introduce latency between collection, detection and response. Since many plant pests are frequently consumed by predators, molecular dietary [...]

Cryptic diversity constrains biogeographical inference in microscopic animals: evidence from bdelloid rotifers in Greenland

Daniel Stec, Filip Matura, Marco Antonio Jiménez Santos, et al.

Published: 2026-06-01
Subjects: Life Sciences, Other Ecology and Evolutionary Biology, Terrestrial and Aquatic Ecology, Zoology

Species are fundamental units of biodiversity, yet their delimitation remains challenging in many organismal groups. The increasing use of DNA data has revealed widespread cryptic diversity, in which genetically distinct lineages are morphologically indistinguishable. Consequently, many morphology-based biogeographical inferences have likely overestimated species ranges, particularly in [...]

The Mother’s Dilemma: Ancient maternal trade-offs explain egalitarian moral cooperation.

Ben Thomas Gleeson

Published: 2026-06-01
Subjects: Biological and Physical Anthropology

The emergence of egalitarian moral norms is widely regarded as a crucial transition in human social evolution, yet it remains a puzzle. Existing explanatory models leave several issues unresolved and share a common reliance on male social agency and alliance-making, which predisposes them towards mechanisms based in social competition. By contrast, drawing on comparative panin socioecology, this [...]

Deterministic by design: causal inference challenges for ‘biodiversity change’ syntheses

Rebecca Spake, Richard McElreath, Luke C Evans

Published: 2026-06-01
Subjects: Ecology and Evolutionary Biology

Understanding biodiversity change over space and time is a central goal of ecology. A common approach to asking why biodiversity is changing over time, is to collate biodiversity time series from multiple sites and undertake a two-step analysis: First, site-level estimates of ‘biodiversity change’ are calculated. Second, those change estimates are regressed on putative environmental drivers, such [...]

A simple demographic explanation for the evolution of the dietary restriction response and its ecological relevance

Joel L Pick, Yimen Gerardo Araya Ajoy, Hannah Froy, et al.

Published: 2026-06-01
Subjects: Ecology and Evolutionary Biology, Life Sciences

Considerable life history plasticity is observed in response to variation in food availability and composition. This is perhaps best known in the context of dietary restriction, which consistently induces lower reproduction and higher survival across taxa, with nutritional geometry studies further demonstrating the importance of food composition as well as amount. Although there is a huge amount [...]

The coevolution of cooperation and socially-mediated dispersal: a model

Iris Prigent, Charles Mullon

Published: 2026-06-01
Subjects: Behavior and Ethology, Evolution, Population Biology

Limited dispersal can promote the evolution of cooperation by increasing relatedness between social partners. However it also intensifies kin competition, potentially cancelling the benefits of helping. Here, we analyse a model in which individuals evolve both (i) the probability of cooperating within social groups as adults, and (ii) the dispersal probability of juveniles conditional on the [...]

Do the benefits of hybridization outweigh the costs under conditions of global change?

Patrick Krapf, Thomas Blankers, Jonna Kulmuni

Published: 2026-06-01
Subjects: Biodiversity, Ecology and Evolutionary Biology, Life Sciences

Global change is predicted to facilitate hybridization but whether the hybrid populations persist and shape biodiversity remains unknown. At the grey zone, before speciation is completed, hybridization is likely leading to simultaneous costs and benefits for hybrid fitness. Whether the benefits outweigh the costs depends on the environment, as hybrid fitness, and potential incompatibilities, can [...]

Still Money for Nothing? Two Decades of Empirical Evaluation of Conservation Investments

Alex Caruana, Joseph W Bull, Paul J Ferraro, et al.

Published: 2026-05-29
Subjects: Life Sciences, Social and Behavioral Sciences

Twenty years ago, the landmark paper “Money for Nothing?” argued that biodiversity conservation relied too little on empirical evidence. It called for more evaluations of conservation effectiveness based on explicit counterfactuals, comparing observed outcomes with those that would likely have occurred in the absence of intervention. To assess progress towards this goal, we systematically [...]

Disentangling the global drivers of species use and use-driven extinction risk

Oscar Morton, Sharon Baruch-Mordo, Christopher Cooney, et al.

Published: 2026-05-29
Subjects: Biodiversity, Life Sciences

The use and trade of wild species is a cornerstone of subsistence livelihoods and global economies. Understanding which drivers of use threaten species is critical in distinguishing between beneficial sustainable exploitation and harmful overexploitation, and ensuring that conservation efforts are targeted where they are most urgently needed. We provide the first nuanced global assessment of [...]

Sex-reversed males are morphologically similar but have lower testosterone levels compared to sex-concordant males in the agile frog

Nadine Lehofer, Veronika Bókony, Andrea Kásler, et al.

Published: 2026-05-29
Subjects: Ecology and Evolutionary Biology

Environment-induced sex reversal – the mismatch between genetic and phenotypic sex caused by external conditions during early development – is increasingly documented in ectothermic vertebrates, yet its fitness-related consequences in natural populations remain poorly tested. To address this gap, we compared sex-reversed XX males and sex-concordant XY phenotypic males in agile frogs (Rana [...]

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