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Preprints

There are 3069 Preprints listed.

Long-term annual seed production data of individual European beech (Fagus sylvatica) trees in the Netherlands

Cherine C. Jantzen, Marcel E. Visser

Published: 2026-03-24
Subjects: Life Sciences

Seed production of European beech (Fagus sylvatica) is highly variable between years and synchronised between individual trees (i.e., masting), creating years with high seed crops, separated by one or more years with little or no seed production. This pulsed reproduction has selective benefits for the trees, as it creates a cycle of predator satiation and starvation, and this way masting drives [...]

Foundational AI could usher in a new era for models of all life on Earth

Joseph Millard, Arman Pili, Katherine Berthon, et al.

Published: 2026-03-24
Subjects: Artificial Intelligence and Robotics, Ecology and Evolutionary Biology

Deep-learned foundational AI could usher in a new era for the simulation of whole ecosystems, argue Joseph Millard and colleagues.

Masting breakdown in European beech reduces fitness benefits of masting, partly explained by climate change

Cherine C. Jantzen, Joseph B. Burant, Marlène Gamelon, et al.

Published: 2026-03-24
Subjects: Life Sciences

1. Masting, which corresponds to highly synchronized but temporally variable seed production, is initiated by weather cues and is thus highly sensitive to climate change. Changes in these cues can lead to a masting breakdown, resulting in a reduction of the fitness benefits of masting through decreasing pollination efficiency and increasing predation risk for seeds. 2. Here, for the first time, [...]

Socio-sexual cues shape female diet choice in Drosophila melanogaster

Mabel Sydney, Jen Perry, Tracey Chapman

Published: 2026-03-24
Subjects: Life Sciences

Male harassment can disturb female feeding behaviour and limit females’ access to preferred foraging locations. However, it is not yet known how females trade off costs of sexual harassment or increased intrasexual competition against preference for dietary macronutrients when making foraging decisions. We used the fruit fly Drosophila melanogaster to investigate how female foraging decisions [...]

Habitat amount control is necessary but not sufficient to resolve the fragmentation debate

Juan Andrés Martínez Lanfranco

Published: 2026-03-24
Subjects: Life Sciences

Opposing conclusions from the same global multi-taxa dataset have intensified debate over whether fragmentation effects can be inferred independently of habitat amount in observational landscape studies. Gonçalves-Souza et al. (2025) reported lower local- and landscape-scale diversity in fragmented landscapes, whereas Fahrig et al. (2026) reanalysed the same dataset with continuous, scale-matched [...]

Microbial inoculants for soil restoration: A Practical Framework for Risk-Governed Stewardship

Gwyn Beattie, Anna Edlund, Joana Falcao Salles, et al.

Published: 2026-03-24
Subjects: Life Sciences

Global soil degradation and increasing reliance on chemical inputs threaten agricultural sustainability, driving interest in microbial inoculants as tools for soil restoration. These biological products have the potential to enhance nutrient cycling, improve soil structure, and support plant resilience, but their environmental release raises important safety and stewardship considerations. Here, [...]

Coexistence of phenotypic plasticity and habitat use in natural populations

Samantha Clare Patrick, Julien G. A. Martin, anne charmantier, et al.

Published: 2026-03-23
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Evolution, Life Sciences

When studying how individuals adapt to environmental changes, the environment is traditionally viewed as a passive backdrop, with individuals modifying their phenotype in response to environmental conditions (i.e., phenotypic plasticity). However, this perspective overlooks the active role of habitat choice in mediating individual responses to environmental changes. In this paper, we argue for [...]

Genetic variance and phenotypic selection on pathogen-linked oviposition choice in Drosophila

Cara Duffy, Qurratu'Aina Abdul Munir, Pedro F Vale

Published: 2026-03-23
Subjects: Animal Experimentation and Research, Behavior and Ethology, Entomology, Evolution, Genetics, Integrative Biology, Research Methods in Life Sciences, Zoology

Pathogen-avoidance behaviour is assumed to be adaptive, yet its phenotypic variability and genetic heritability are rarely quantified. In species lacking post-oviposition care, avoiding potentially infectious egg-laying substrates would improve offspring survival and should therefore be under strong selection. We used two-choice oviposition assays to quantify the phenotypic and genetic variance [...]

Direct and biodiversity-mediated effects of climate on grassland productivity across the Alps

Pierre Gauzere, Maria Hällfors, Jussi Mäkinen, et al.

Published: 2026-03-23
Subjects: Ecology and Evolutionary Biology, Life Sciences

Understanding how climate shapes ecosystem productivity through both energetic constraints and biodiversity‑mediated pathways remains a major challenge in global change ecology, particularly in mountain grasslands where rapid warming and strong environmental gradients interact. Here, we disentangle and map direct climatic controls on productivity from indirect effects mediated by canopy [...]

Heritabilities and genetic correlations of Drosophila melanogaster locomotory behaviour traits: a high-throughput phenotyping approach

Szymon Marian Drobniak, Erian Macartney, Samantha Burke, et al.

Published: 2026-03-23
Subjects: Behavior and Ethology, Evolution

Genetic variance forms the basis for evolutionary inferences as it describes the evolutionary potential of traits. The major limitation of quantitative genetic studies is achieving sufficient power and sample sizes to estimate heritabilities with sufficient precision. This issue is especially important in the case of traits that are inherently susceptible to stochastic, nonbiological variation. [...]

High Data Quality Enhances Microplastic Toxicity Prediction

Ana L. Antonio Vital, Scott Coffin, Andrea Bonisoli-Alquati, et al.

Published: 2026-03-23
Subjects: Life Sciences

Unlike chemicals, microplastics (MPs) lack standardized identifiers, limiting the applicability of traditional predictive ecotoxicology methods such as quantitative structure-activity relationship (QSAR) models. This study aimed to predict MP toxicity using MP properties, MP concentration, organismal traits, endpoints, and experimental design, and to evaluate how data pre-processing, dataset [...]

Synergies and trade-offs between tree cover expansion efforts within and outside forests to achieve climate, biodiversity and human well-being outcomes

Pooja Choksi, Jennifer S. Powers, Laura Toro, et al.

Published: 2026-03-23
Subjects: Biodiversity, Environmental Studies, Nature and Society Relations, Other Forestry and Forest Sciences, Physical and Environmental Geography, Remote Sensing

Increasing tree cover is hailed as a leading climate mitigation strategy, yet there is increasing evidence that there may be trade-offs between trees in different parts of the landscape. Existing science, policy, and practice on natural climate solutions (NCS) assumes that trees outside of forests, for example on farms, homesteads, or in urban areas, are synergistic with forest conservation: if [...]

A robust method for quantifying the contribution of transient dynamics to variation in population growth rate

Christina Maria Hernandez, Harman Jaggi, James Cant, et al.

Published: 2026-03-20
Subjects: Population Biology

Understanding why population growth rates vary through time is central to ecology, evolution, and conservation. In structured populations, such variation arises from both environmentally-driven fluctuations in vital rates and intrinsic transient dynamics generated by changes in population structure. Despite long-standing recognition of these processes, existing approaches do not provide an exact [...]

Missing the mammals for the trees: comparative biogeography of southern Appalachian sky island biodiversity

Kimberly July Cook, David Webster, Juliet Stowe, et al.

Published: 2026-03-20
Subjects: Life Sciences

Sky islands are patches of relic ecosystems on mountaintops, often forest types, that were previously connected in the geological past and are now patchily distributed. Whether a particular sky island forest serves as an umbrella for conserving sky island fauna with a similar patchy distribution is an open question. To address this, we systematically delineate sky islands and identify [...]

Insect oviposition site selection as a simple system to investigate the ecology and evolution of pathogen avoidance behaviour

Pedro F Vale, Cara Duffy

Published: 2026-03-20
Subjects: Animal Experimentation and Research, Behavior and Ethology, Biology, Entomology, Evolution, Immunology and Infectious Disease, Integrative Biology, Laboratory and Basic Science Research Life Sciences, Research Methods in Life Sciences, Zoology

Pathogen avoidance is a form behavioural immunity and provides the first line of defence from infection. This article discusses insect oviposition as a tractable, mechanistically grounded model for behavioural immunity and sets a concrete research agenda linking individual egg-laying decisions to population level disease dynamics. Because egg laying decisions determine offspring exposure to [...]

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