Preprints
There are 2721 Preprints listed.
Resurrection of Anopheles darlingi FREP1 Ancestor Reveals Adaptive Evolution Characterized by Changes in Protein Stability and Plasmodium falciparum Interaction
Published: 2025-11-21
Subjects: Life Sciences
Fibrinogen-related protein 1 (FREP1), a midgut-localized fibrinogen-like lectin in Anopheles mosquitoes, mediates Plasmodium ookinete attachment by binding α-tubulin-1. To elucidate the evolutionary forces shaping this interaction, the study analysed FREP1 sequences from 29 Anopheles species using codon-based tests, ancestral sequence reconstruction, stability modelling, and docking. Both aBSREL [...]
RNA Virus Discovery from Daphnia meta-transcriptomes: A novel Tombunoda-like virus based on RNA dependent RNA polymerase identification
Published: 2025-11-21
Subjects: Bioinformatics, Life Sciences, Virology
The discovery of RNA viruses from meta-transcriptomes has led to an explosion in viral diversity and identified many novel host-virus associations. To date, no studies have examined the RNA virome of the model zooplankter Daphnia. From which only four viruses are known, with only one being well understood. Here, I assemble and annotate RNA-dependent RNA polymerase (RdRp) containing contigs from [...]
Changes in the fire resilience of Mediterranean trees in response to climate variability over the past 300 Years
Published: 2025-11-21
Subjects: Engineering, Life Sciences
With global warming, understanding the tree resilience to fire is still a scalding topic in ecology. Tree resilience would depend on fire intervals, and on interactions with atmospheric conditions, including temperature, precipitation, drought, and CO₂. These interactions would be exacerbated in Mediterranean ecosystems. We test this hypothesis by analysing variations in tree-ring widths [...]
The resurgence of Evening Grosbeak Coccothraustes vespertinus: A 2024-2025 winter revival in Boileau, Québec: A Research Study
Published: 2025-11-21
Subjects: Life Sciences
The Evening Grosbeak is widely distributed across Canada’s forests but has exhibited significant long-term declines (77-90%) over most of its range since 1970. Project FeederWatch data gathered between 1988 and 2006 indicated a significant survey wide decline in the mean flock size, 27% in 18 years. The number of sites reporting any sighting plunged 50%. Breeding bird survey (BBS) data indicate [...]
Reconciling top-down conservation priorities with bottom-up local needs
Published: 2025-11-21
Subjects: Community-based Research, Environmental Policy, Life Sciences, Policy Design, Analysis, and Evaluation
The success of global conservation goals risks being undermined by conflicts that arise when high-level, data-driven priorities clash with local needs and contexts. While top-down systematic planning efficiently identifies priority areas using large-scale, multi-dimensional data, it neglects the input of local communities and stakeholders. Here, we propose a novel priority-setting process that [...]
What evidence exists on the effectiveness of nature-inclusive designs for marine biodiversity in offshore wind farms? A Systematic map protocol
Published: 2025-11-21
Subjects: Biodiversity, Life Sciences, Marine Biology
The global expansion of offshore wind energy is essential for meeting climate targets, yet its ecological impacts on marine biodiversity remain a major concern. In line with the Kunming–Montreal Global Biodiversity Framework, which calls upon all economic sectors to reduce negative impacts and enhance positive contributions, Nature-inclusive Designs (NiDs) have been proposed to reduce the [...]
Oxidative stress after pollutant exposure depends strongly on experimental design and pollutant properties: a meta-analysis
Published: 2025-11-21
Subjects: Life Sciences
Measurements of reactive oxygen species (ROS) are often performed to assess a species’ general sublethal stress response to a pollutant. However, ROS bioassays often produce seemingly ambiguous results, and the drivers that lead to these differences are largely unknown. To approach this gap, we conducted a meta-analysis on ROS generation, ROS-associated damage products, enzyme activities, and [...]
Functional stability of coastal plankton communities toward extreme weather-related stressors
Published: 2025-11-19
Subjects: Life Sciences
Extreme weather events, such as marine heatwaves and terrestrial runoff, are intensifying in many areas and pose growing threats. In the present study, we assessed the functional stability of plankton communities in response to these events by comparing their responses to marine heatwave and terrestrial runoff stressors. Using two in situ mesocosm experiments conducted at the same coastal site, [...]
Edge-of-range camera-trap records of Superb Lyrebird (Menura novaehollandiae) in western and central-north Tasmania (2018–2025)
Published: 2025-11-18
Subjects: Biodiversity, Life Sciences, Ornithology
Tasmania’s Superb Lyrebird (Menura novaehollandiae) was deliberately introduced to south‑east Tasmania in 1934 and has since dispersed across much of the island’s central bioregions. Despite this expansion, the Lyrebird’s future range dynamics remains uncertain, with recent modelling projecting that it will take over 50 years for the species to establish in the north-west of the island. Here we [...]
Humanity’s redistribution of global biomass flows
Published: 2025-11-18
Subjects: Ecology and Evolutionary Biology, Life Sciences
The biosphere is connected by flows of organic material (biomass), through biogenic (e.g., animal migration) or anthropogenic pathways (e.g., trade). We argue that humans have drastically altered Earth’s biomass flows by disrupting animal movement, directly transporting biomass and creating novel biotic pathways. In 2023, transnational anthropogenic transport of biomass through trade far exceeded [...]
Sex-specific mutation accumulation: A parsimonious explanation for sex differences in lifespan and ageing
Published: 2025-11-17
Subjects: Life Sciences
Sex differences in lifespan and ageing pervade the tree of life, yet their evolutionary origin is still debated. Adaptive trade-off models have long dominated the field but show mixed empirical support. Here we argue that sex-specific mutation accumulation is the most parsimonious evolutionary cause of sex-biased ageing. Because anisogamy and ecology shape reproductive and survival schedules, [...]
Bioclimatic modelling of the spread of Dirofilaria spp. in Europe, with a special focus on Ukraine
Published: 2025-11-17
Subjects: Life Sciences
Background: The zoonotic disease dirofilariosis, caused by Dirofilaria spp., is expanding geographically in Europe, a phenomenon increasingly linked to climate change. Understanding the environmental drivers of this spread is crucial for surveillance and public health planning. Objective: This study aims to model the ecological niche of Dirofilaria spp. in Europe, identify key climatic drivers, [...]
Response to “Radiolarian evolution: Analytical challenges in estimating the diversity and origin of Nature’s stars”
Published: 2025-11-14
Subjects: Biodiversity, Ecology and Evolutionary Biology, Environmental Microbiology and Microbial Ecology Life Sciences, Genetics and Genomics, Marine Biology
We appreciate Daniel Lahr’s concern (Lahr, 2025) on the interpretation and conclusions of our study “Extant diversity, biogeography, and evolutionary history of Radiolaria” (Sandin et al., 2025). Given that some of these comments were already addressed in our original study following reviewers comments and that such issues are well known in the molecular diversity and evolution fields we [...]
Microevolutionary consequences of social structure in wild spotted hyenas
Published: 2025-11-14
Subjects: Life Sciences
Social structure - arising from non-random associations and interactions among conspecifics - is a defining feature of most animal populations, yet evolutionary theory typically assumes genetic and social homogeneity. This disconnect limits our ability to predict how natural populations evolve. We combined nearly 30 years of behavioural, life-history, and genomic data from wild spotted hyenas [...]
Dispersion tests in generalised linear mixed-effects models - a methods comparison and practical guide
Published: 2025-11-14
Subjects: Applied Statistics, Ecology and Evolutionary Biology, Statistical Models
1. Underdispersion and overdispersion are common issues when analysing ecological data with generalised linear (mixed) models (GLMs/GLMMs). Overdispersion, the phenomenon where observations spread wider than expected by the fitted model, leads to anti-conservative p-values and, thus, to inflated type I error. In contrast, underdispersion, a narrower spread of the data than expected, causes overly [...]