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Preprints

There are 3266 Preprints listed.

A Critical Year for Nature: Now is the time to accelerate action on the Global Biodiversity Framework

E.J. Milner-Gulland, Éilish Farrelly, Talitha Bromwich, et al.

Published: 2026-05-20
Subjects: Biochemistry, Biophysics, and Structural Biology, Biodiversity, Environmental Policy, Environmental Studies

In October 2026, Parties to the United Nations Convention on Biological Diversity (CBD) will convene to review progress against the Kunming-Montreal Global Biodiversity Framework (KMGBF) adopted in 2022. A global report will provide a summary of collective progress, primarily drawing from government self-reporting at the halfway mark to 2030, the target year for halting and reversing nature loss. [...]

Collembola eco-morphological indices (EMI) and Soil Biological Quality Index (QBS-c): a review and practical guidelines for soil health assessment

Martina Coletta, Antonietta La Terza, Stefan Scheu, et al.

Published: 2026-05-20
Subjects: Biodiversity, Ecology and Evolutionary Biology

Soil health assessments remain dominated by physicochemical indicators, largely due to limited functional understanding and a lack of practical tools for quantifying soil biodiversity in applied contexts. However, many soil functions are fundamentally driven by biotic components, highlighting the need for robust biological indicators. The Soil Biological Quality indices, QBS-ar and QBS-c (Parisi, [...]

The holobiont is not a useful model for most host-microbiome interactions

Gavin M Douglas, S. Andrew Inkpen

Published: 2026-05-20
Subjects: Environmental Microbiology and Microbial Ecology Life Sciences, Evolution, Life Sciences

The holobiont concept refers to a host and associated microbes. It has been critiqued over the last decade, primarily based on the argument that individual holobionts are not an appropriate level for analyzing multi-generation host dynamics, as most microbes are acquired from the environment. Several responses were given to this and other criticisms. The main response has been that the holobiont [...]

Fishers’ local knowledge strengthens seagrass restoration planning

Benjamin Lawrence Hopper Jones, Flo Taylor, Emma Fox, et al.

Published: 2026-05-20
Subjects: Biodiversity, Ecology and Evolutionary Biology, Marine Biology, Natural Resources and Conservation, Natural Resources Management and Policy, Social and Behavioral Sciences

Seagrass restoration is increasingly guided by habitat suitability models, yet restoration outcomes depend on more than biophysical suitability alone. In coastal social-ecological systems, fishers and anglers hold fine-scale, time-integrated knowledge of habitat condition, human use, and local constraints that are rarely incorporated at the outset of restoration planning. Here, we tested whether [...]

Insufficient environmental protection by the European regulatory framework for pesticides

Ralf B. Schäfer, Thomas Backhaus, Juliane Filser, et al.

Published: 2026-05-20
Subjects: Agriculture, Environmental Health Life Sciences, Terrestrial and Aquatic Ecology, Toxicology

Schriever et al. (2025) argue that environmental risk assessment of pesticides in the European Union is sufficiently protective and that regulatory thresholds are rarely exceeded. Here, we re-examine these claims based on new and previous evidence from monitoring, systematic reviews, and different types of field studies. The clear outcome is that measured pesticide concentrations frequently [...]

Multi-eared Listening: Pathways to Equitable Partnerships in a Transdisciplinary Ecoacoustics

Alice Eldridge, Leah Barclay, Grant Smith, et al.

Published: 2026-05-20
Subjects: Arts and Humanities, Life Sciences

Multi-Eared Listening is a call to integrate Indigenous, local, and Western scientific knowledges in a transdisciplinary ecoacoustic research. Following the Mi’kmaw principle of Two-Eyed Seeing, Multi-Eared Listening, points to the importance of braiding epistemologies in ecoacoustic research and conservation. Case studies from Ecuador, Ghana, Indonesia, Australia, and transnational sound art [...]

A financial framework for nature must that recognise that ecosystems have local temporal and spatial dynamics

William D Pearse, Franklin Allen, Simone Cenci, et al.

Published: 2026-05-19
Subjects: Biodiversity, Business

Decades of research and politics have led to a coherent set of biodiversity metrics and strong evidence that biodiversity supports human well-being. So why do we not have a financial system that encourages conservation and restoration of nature? We argue that a central reason is that the financing used has not been aligned with the spatial and temporal structure of biodiversity. Ecosystems are [...]

Colossal Disinformation

Vincent Lynch

Published: 2026-05-19
Subjects: Biology, Ecology and Evolutionary Biology, Life Sciences

I have a position: de-extinction is almost impossible, however, deëxtinction, a word invented by the world’s first “de-extinction” company Colossal Laboratories & Bioscience’s can be (Max 2025); hereafter Colossal, which they define as the creation of genetically modified organisms (GMOs) that appear and act like extinct species, is possible. It’s branding, not science. I and other critics of [...]

The roles of density dependence, developmental asynchrony, and niche traversal costs in shaping the evolution of ontogenetic complexity

James G DuBose

Published: 2026-05-19
Subjects: Life Sciences

Most organisms undergo some degree of niche transition throughout their life cycles, which are typically accompanied by morphological, physiological, and/or behavioral changes. Ontogenetic complexity generally refers to the magnitude and abruptness of these changes. Evolutionary theory has described how various genetic properties facilitate and constrain the evolution of ontogenetic complexity, [...]

A Protocol for Standardizing Measurements and Enabling Global Harmonization of Herbarium Leaf Reflectance Spectra

Dawson M White, Flávia M Durgante, Matthew Austin, et al.

Published: 2026-05-19
Subjects: Biochemistry, Biophysics, and Structural Biology, Biodiversity, Bioinformatics, Botany, Ecology and Evolutionary Biology, Life Sciences, Other Ecology and Evolutionary Biology, Research Methods in Life Sciences

Reflectance spectroscopy offers a powerful approach to integrate high-throughput phenotypic data from herbarium specimens into the digital landscapes of ecology, evolution, and systematics. Because inconsistencies in instrumentation and measurement practices can increase noise and limit dataset compatibility, the International Herbarium Spectral Digitization Working Group (IHerbSpec) has [...]

Cheating and imperfect vaccines as drivers of bacterial evolution

Florian Lecorvaisier, Thomas Louis Philippe Martin

Published: 2026-05-19
Subjects: Life Sciences

Cheating is an ubiquitous evolutionary strategy, appearing everywhere throughout the tree of life. Among bacteria, cheating appears mostly through the consumption of public good without participation in their production. Some vaccines, because they specifically target these public goods, may alter the eco-evolutionary dynamics of cheating in bacterial populations of pathogenic species such as [...]

Oak masting remains stable despite climate warming

Michał Bogdziewicz, Jessie Foest, Jakub Szymkowiak, et al.

Published: 2026-05-19
Subjects: Ecology and Evolutionary Biology, Life Sciences

Climate warming alters tree reproduction, but long-term tests of how it affects the large, synchronised interannual fluctuations in seed production known as masting remain rare. Theory predicts that climate-driven declines in masting should be most likely when species combine high reproductive sensitivity to weather cues with rapid climatic change during the corresponding cue windows. We tested [...]

Parasites and forage as determinants of body condition and population size in an imperiled ungulate.

Benjamin Juan Padilla, Oscar Alejandro Aleuy, Petter Jacobsen, et al.

Published: 2026-05-19
Subjects: Life Sciences, Other Ecology and Evolutionary Biology, Parasitology, Population Biology

Rapid environmental changes are resulting in widespread changes in population size, health, and physiology of wildlife, especially at northern latitudes where the impacts of climate change are more pronounced. Barren-ground caribou (Rangifer tarandus groenlandicus) have declined across much of their range in recent decades, and while the ultimate causes are unknown, western science and local [...]

Quantitative Metabarcoding for Invertebrate Pest Monitoring and Management

Lachlan J. Gretgrix, Jack L. Scanlan, Francesco Martoni, et al.

Published: 2026-05-19
Subjects: Agriculture, Entomology, Molecular Genetics

Invertebrate pests are one of the most significant threats to global agriculture. To monitor these pests, invertebrate trapping methods are commonly used to collect a representation of the diversity of pest species present in the ecosystem. Assessing and monitoring such diversity is key to inform pest management strategies, but due to the complexity of bulk trap samples from non-selective [...]

Designing for nature doesn't cost the Earth

Jacinta Ellesse Humphrey, Holly Louise Kirk, Victoria Cook, et al.

Published: 2026-05-19
Subjects: Biodiversity, Life Sciences, Terrestrial and Aquatic Ecology, Urban Studies and Planning

1. A key barrier to the development of nature positive cities is the unknown cost of implementing novel urban design elements. Strict budgets and government approval processes make it challenging for developers to trial new approaches, meaning most developments rarely deviate from ‘business-as-usual’ (BAU). Further research is urgently needed to overcome this barrier to innovation and help [...]

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