Preprints
Filtering by Subject: Ecology and Evolutionary Biology
Expanding the sentinel approach through multimodal integration: resolving underlying ecological processes with eDNA and computer vision
Published: 2026-05-19
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Life Sciences, Other Ecology and Evolutionary Biology, Research Methods in Life Sciences, Terrestrial and Aquatic Ecology
Sentinel approaches provide a semi-controlled method for quantifying in-field ecological interactions and processes while reducing bias and labour. They are, however, limited by difficulties ascribing taxonomic identities, behavioural context and temporal resolution to interacting agents. The integration of additional sources of data, including the analysis of DNA left behind on sentinel objects [...]
Ecoregion drives intraspecific variation of secondary chemistry in a big sagebrush common garden
Published: 2026-05-19
Subjects: Ecology and Evolutionary Biology
Phytochemistry plays an integral role in the health and survival of plants and can mediate community interactions, communication with neighboring plants, protection against disease and herbivores, and attraction of pollinators. We measured non-volatile compounds using liquid chromatography-mass spectrometry (LC-MS) and volatile compounds using gas chromatography (GC) at two different time points [...]
Removal and decomposition of fruit respond in opposite ways to canopy cover in a biodiversity experiment
Published: 2026-05-14
Subjects: Ecology and Evolutionary Biology, Entomology
Trees often produce more fruits than frugivores consume. As a result, many fruits fall to the ground, where they are either secondarily removed by vertebrates, potentially leading to seed dispersal, or they are decomposed by arthropods. Although often neglected, fallen fruits are an important component of forests and contribute to their functioning via these two distinct pathways. While fruit [...]
The bacterial immune system: identifying evolved defense adaptations
Published: 2026-05-13
Subjects: Ecology and Evolutionary Biology, Evolution, Microbiology
The last few years have witnessed a rapid expansion of reported bacterial defense mechanisms. Alongside established mechanisms of defense against molecular parasites (e.g. CRISPR-Cas, restriction-modification), hundreds of novel defenses are being described each year, contributing to an ever-expanding ‘bacterial immune system’. Terms like ‘defense’ and ‘immune’ are often used as shorthand for an [...]
Cold treatment benefits Mediterranean orchid seedlings cultivated in vitro
Published: 2026-05-11
Subjects: Biology, Ecology and Evolutionary Biology, Life Sciences, Other Ecology and Evolutionary Biology, Plant Biology, Plant Sciences
1. Effective ex situ propagation is increasingly critical for the conservation and restoration of terrestrial orchids threatened by habitat loss, over-collection and climate change. However, large-scale propagation remains constrained by developmental bottlenecks during the transition from protocorms to established plantlets with storage organs. Cold exposure is often recommended to improve [...]
Hybridization in Animal Evolution
Published: 2026-05-11
Subjects: Ecology and Evolutionary Biology, Life Sciences
In the past two decades, it has become clear that hybridization is so common in animal species as to be an almost universal feature of their evolutionary histories. Remnants of both ancient and contemporary hybridization events are present in the genomes of modern species, but their consequences are still not completely understood. In this review, we synthesize what is known about the [...]
Sexually antagonistic selection: a review of the theory and its implications
Published: 2026-05-08
Subjects: Ecology and Evolutionary Biology, Evolution, Population Biology
Sexually antagonistic selection arises when females and males have different fitness optima for traits with a shared genetic basis, so that the same alleles are favoured in one sex but disfavoured in the other. It has been implicated in a wide range of ecological and evolutionary processes, from the maintenance of a sex load to the evolution of sex chromosomes. Mathematical models have long been [...]
Optimizing sampling and monitoring of species interactions within Biodiversity Observation Networks
Published: 2026-05-08
Subjects: Biodiversity, Ecology and Evolutionary Biology
Optimal monitoring strategies should be designed to efficiently monitor all essential facets of biodiversity. Yet, species interactions are often overlooked in monitoring designs compared to spatial coverage and species richness, partly due to the inherent difficulty of sampling and monitoring interactions compared to species distributions. Here, we used simulations to test the efficiency of [...]
Invasiveness reshapes the historical pattern of carp trait evolution
Published: 2026-05-07
Subjects: Ecology and Evolutionary Biology, Life Sciences
Human-mediated invasions are increasingly recognised as contemporary ecological disturbances with profound impacts on microevolutionary processes. However, whether such impacts extend beyond microevolutionary change to alter long-term evolutionary trajectories across lineages remains poorly explored. Using a global phylogenetic analysis of nearly 1,400 carp species (freshwater fishes of the [...]
Identifying knowledge gaps on simultaneous above- and belowground organism responses to global change
Published: 2026-05-07
Subjects: Ecology and Evolutionary Biology, Life Sciences, Terrestrial and Aquatic Ecology
Global change affects all terrestrial organisms regardless if they live above or below the ground. Even though they are strongly linked by various direct and indirect interactions, organisms can respond very differently to global change stressors, due to different features of above- and belowground compartments. Many different organism groups have shown declines in diversity, abundance or biomass [...]
Multi-scale surveillance reveals substantial egg-laying winter activity in Aedes albopictus mosquito populations across temperate Europe
Published: 2026-05-07
Subjects: Ecology and Evolutionary Biology, Entomology, Life Sciences
The Asian tiger mosquito (Aedes albopictus), a competent vector for dengue, chikungunya, and Zika viruses, has expanded rapidly across temperate Europe. European vector surveillance typically operates May to October, assuming winter diapause precludes activity and transmission risk. However, recent field observations suggest sustained egg-laying winter activity in southern European populations, [...]
Assessing the sensitivity and robustness of the Living Planet Index through simulated population dynamics: strengths, stability, and challenges
Published: 2026-05-07
Subjects: Animal Sciences, Biodiversity, Biology, Ecology and Evolutionary Biology, Forest Sciences, Life Sciences
Understanding population change through time is crucial for effective conservation of biodiversity. The Living Planet Index (LPI) is a key indicator for tracking global species abundance trends under the Kunming-Montreal Global Biodiversity Framework, offering a picture of population change over time. However, the sensitivity of the index to zero values or to the number of missing values in time [...]
The evolutionary link between food, condiments and medicine
Published: 2026-05-06
Subjects: Agricultural Science, Anthropology, Biodiversity, Biology, Botany, Ecology and Evolutionary Biology, Evolution, Food Science, Life Sciences, Plant Biology, Plant Sciences, Social and Behavioral Sciences
The deep relationship between humans and plants is of great interest to ethnobotanists, human ecologists, and evolutionary biologists. Humans have incorporated thousands of plant species into both traditional medicine and our diets, as foods and condiments. Many of these provide not only calories but also micronutrients and other bioactive compounds that contribute to health [1]. The boundaries [...]
Making survival spatial: an integrated model for territory occupancy and capture-recapture data
Published: 2026-05-05
Subjects: Biology, Ecology and Evolutionary Biology, Population Biology
Knowledge about spatial variation in survival is central to understanding population dynamics and guiding conservation, yet assessing it is very hard. This limitation arises because capture-mark-recapture (CMR) data required for such inference must be collected over large spatial extents, which is logistically demanding and seldom possible. By contrast, territory occupancy (TO) data are typically [...]
Does the substrate on which cryptogams grow matter for limno-terrestrial meiofauna?
Published: 2026-05-04
Subjects: Biology, Ecology and Evolutionary Biology, Life Sciences, Other Ecology and Evolutionary Biology, Terrestrial and Aquatic Ecology
Cryptogam habitats support a wide range of limno-terrestrial meiofauna, but the factors that shape their communities are still not well understood. The physical substrate that cryptogams grow on (e.g., soil, the base of a tree, or its trunk) can influence local moisture, temperature, and nutrient conditions, yet its role in structuring meiofaunal assemblages has rarely been tested systematically. [...]