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Preprints

Filtering by Subject: Ecology and Evolutionary Biology

Density dependence buffers natural populations against multiple stressors

Man Qi, Rob Salguero-Gomez, Àlex Giménez Romero, et al.

Published: 2026-08-14
Subjects: Ecology and Evolutionary Biology, Life Sciences

Predicting population persistence under global change requires understanding how populations buffer against interacting stresses. Gradual stresses, such as droughts, suppress individual performance, whereas abrupt stresses, such as harvesting, remove individuals from populations. Because these stresses interact through density-dependent responses of growth, reproduction and survival, predicting [...]

Plant sex chromosome evolution in the phylogenomic era

Mark Hibbins, Stephen Wright

Published: 2026-08-13
Subjects: Ecology and Evolutionary Biology, Evolution, Genetics and Genomics, Life Sciences, Plant Sciences

Plants harbor a tremendous diversity of sexual systems, including various forms of chromosomal sex determination that have arisen repeatedly across major clades, especially in angiosperms. The causes and consequences of sex chromosome evolution are far-reaching, with implications for sexual system, mating system, and life history evolution, genome architecture, and speciation, extinction, and [...]

Lake Champlain fisheries and ecosystem in the context of the Laurentian Great Lakes: past research, current management issues, and future research priorities

Ellen Marsden, Thais A. Bernos, Jason D Stockwell

Published: 2026-08-11
Subjects: Ecology and Evolutionary Biology, Life Sciences

Lake Champlain is a large lake bordered by New York, Vermont, and Quebec. This bi-national, multi-jurisdictional lake shares many management issues and challenges with the much larger bi-national Laurentian Great Lakes, including a history of commercial fisheries, species declines and extirpations, sport fisheries supported by stocking, and invasion of non-native species. The bathymetric [...]

Chromosomal speciation through holocentric lenses

Inés Gómez-Ramos, Rogelio Sánchez-Villegas, Ana Valdés-Florido, et al.

Published: 2026-08-11
Subjects: Ecology and Evolutionary Biology, Evolution, Life Sciences

Chromosomal rearrangements (CRs) can trigger speciation by acting as barriers to gene flow. Two main theoretical frameworks were developed on how they contribute to speciation: causing infertility in heterokaryotypes (hybrid dysfunction) or creating islands of divergence through recombination suppression. However, these theories were developed with monocentric chromosomes in mind. Species with [...]

Beyond the mammalian nucleolus—a comparative perspective on nucleolar dynamics in stress and through the life cycle

Ricardo Carvalho, Elizaveta Gubanova, Gautam Dey

Published: 2026-08-11
Subjects: Cell and Developmental Biology, Cell Biology, Ecology and Evolutionary Biology, Evolution, Microbiology, Organismal Biological Physiology

Classically the site of ribosomal RNA transcription, processing and early ribosome assembly, the nucleolus is increasingly understood as a dynamic multiphase condensate reshaped by cell state. Most reviews of this plasticity are mammal-centred. Here we set mammals alongside fungi, plants and animal germlines, following the nucleolus through the life cycle under environmental change and through [...]

Impacts of the end Pliocene marine megafaunal extinction on North Atlantic trophic structure

Amy Shipley, Andrew P Beckerman, Catalina Pimiento, et al.

Published: 2026-08-11
Subjects: Ecology and Evolutionary Biology

Marine megafauna are experiencing widespread population declines and these losses are expected to have cascading effects, impacting ecosystem structure and function. However, quantifying these ecological consequences at a global scale remains challenging, requiring an understanding of long-term trophic dynamics. Palaeoecology can provide useful insights into how communities respond to taxonomic [...]

Impact of warming on functional traits of a keystone grazer in Arctic lakes

Athina Karapli-Petritsopoulou, Dörthe Becker, Dagmar Frisch

Published: 2026-08-07
Subjects: Comparative and Evolutionary Physiology, Ecology and Evolutionary Biology, Laboratory and Basic Science Research Life Sciences, Terrestrial and Aquatic Ecology

Temperature rise is one of the most pronounced abiotic changes currently affecting Αrctic ecosystems. Knowledge about the adaptation capacity of their key species is urgently needed to better understand the ecological consequences for these sentinel ecosystems. Previous work on a keystone zooplankton grazer, the obligatory parthenogenetic Daphnia pulicaria, from a lake in South-West Greenland [...]

EcoScape: Species-specific Functional Habitat Connectivity Via Efficient Parallel Propagation Simulations

Natalia Ocampo-Peñuela, Luca de Alfaro, Jasmine Tai, et al.

Published: 2026-08-06
Subjects: Ecology and Evolutionary Biology, Other Ecology and Evolutionary Biology, Population Biology

Well-connected habitats sustain viable populations through gene flow, repopulation potential, and functional habitat extent. Conserving and enhancing habitat connectivity has been identified as a priority to halt extinctions and safeguard 30% of the planet. Conservation prioritization would therefore benefit from habitat connectivity modeling that is applicable at broad spatial and taxonomic [...]

Characterization of Haplosporidium pinnae's biogeographical distribution by means of Ecological Niche Modelling techniques and consequences of climate change upon its range

Nicholas Alexander Vaz Reay

Published: 2026-08-06
Subjects: Ecology and Evolutionary Biology

This study provides a biogeographical characterization of protozoan endoparasite Haplosporidium pinnae's distribution following the associated Mass Mortality Event of endangered bivalve Pinna nobilis in 2016. The epizootic has reached mortality rates between 80% - 100% and was first reported in the south-western Mediterranean Sea. It has since spread to north-western waters, followed by the [...]

A Survey of the Terrestrial Angiospermic Indigenous Flora of College of Education, Agbor, Delta State, Nigeria

Evangeline O Chiedu, Bernard O Obadoni, Oseghale E Ehilen, et al.

Published: 2026-08-06
Subjects: Ecology and Evolutionary Biology, Plant Sciences

Seeking to explore the impact of developmental activities on plant biodiversity, a survey was embarked upon to document plant species naturally occurring on the College of Education (now University of Delta), Agbor, Delta State, Nigeria. A total of 48 species were recorded on Campus I, and 54 species were recorded on Campus II, belonging to 20 and 26 families respectively. On Campus I, the family [...]

How (not) to study insect microbiota in the age of high-throughput sequencing

Piotr Łukasik, Karol Hubert Nowak, Raphael Eisenhofer

Published: 2026-08-05
Subjects: Biodiversity, Ecology and Evolutionary Biology, Entomology, Environmental Microbiology and Microbial Ecology Life Sciences

The recognition of microbiota as a critical component of insect biology and evolution has driven a rapid expansion of insect-microbe interaction research. Yet while high-throughput sequencing has democratized access to microbial community profiling, it has also normalized low-resolution, artifact-prone approaches that often yield limited biological insights. Insects pose distinct challenges for [...]

Dark invasions as cryptic components of biological invasions

Jingrui Sun, Teun Everts, Ronaldo Sousa, et al.

Published: 2026-08-05
Subjects: Ecology and Evolutionary Biology, Terrestrial and Aquatic Ecology

Understanding and managing biological invasions typically requires knowledge of species records, known impacts, recognized pathways and definitions of non-native status, yet this information is rarely complete or without uncertainties. We develop the concept of dark invasions to describe a suite of hidden or underacknowledged aspects of biological invasions that are rarely quantified because [...]

Influence of Selected Urban Tree Species on Soil Microbial Activity, Litter Decomposition, and Soil Chemical Properties in Akure, Southwestern Nigeria

Zoe Faith Zebedee, Solomon Adejoro

Published: 2026-08-04
Subjects: Agriculture, Ecology and Evolutionary Biology, Forest Sciences, Other Life Sciences, Plant Sciences

Urban trees shape belowground ecological processes through litter deposition, root exudation, and interactions with soil microbial communities, yet species-specific effects are rarely incorporated into urban green-space management. This study evaluated the effects of five common urban tree species (Eucalyptus globulus, Azadirachta indica, Thuja occidentalis, Prunus dulcis, and Buddleja globosa) [...]

Life-history traits do not always evolve in response to changing thermal environments

Patrice Pottier, Liam R Dougherty, Ramakrishnan Vasudeva, et al.

Published: 2026-08-04
Subjects: Biodiversity, Biology, Comparative and Evolutionary Physiology, Ecology and Evolutionary Biology, Entomology, Integrative Biology, Zoology

Quantifying animals' capacity to evolve in response to changing environments is central to predicting ecological responses to climate change. However, empirical evidence for evolutionary responses to changing temperatures remains mixed, and we know little about how selection alters trait variance or generates trade-offs among traits. Here, we conducted a systematic review and meta-analysis of 44 [...]

Wolf–Deer Movement After the 2021 Wolf Harvest, by North-South Gradient and Proximity to Towns using a Five-County Camera Record (2018–2025)

Damian Anthony Vraniak

Published: 2026-08-03
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Life Sciences

Previous analysis of a wolf-enriched extract of the Snapshot Wisconsin camera network for five northwestern counties (Barron, Burnett, Bayfield, Douglas, and Sawyer; 525 cameras, 294,031 camera-days, 634,214 detections, 2018–2025; 1,856 wolf detections on 197 cameras) established patterns in the way gray wolves (Canis lupus) follow, select which class of deer to follow, and how strongly they [...]

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