Preprints
Filtering by Subject: Ecology and Evolutionary Biology
Sex-reversed males are morphologically similar but have lower testosterone levels compared to sex-concordant males in the agile frog
Published: 2026-05-30
Subjects: Ecology and Evolutionary Biology
Environment-induced sex reversal – the mismatch between genetic and phenotypic sex caused by external conditions during early development – is increasingly documented in ectothermic vertebrates, yet its fitness-related consequences in natural populations remain poorly tested. To address this gap, we compared sex-reversed XX males and sex-concordant XY phenotypic males in agile frogs (Rana [...]
Developmental Plasticity in Response to Temperature Aligns with Allen’s but not Bergmann’s Rule
Published: 2026-05-30
Subjects: Ecology and Evolutionary Biology
Increases in average global temperatures have caused researchers to revisit how species and populations are expected to respond to changing temperatures. Allen’s rule and Bergmann’s rule describe two ecogeographic patterns where species in high (cold) latitudes have shorter limbs and larger bodies, respectively, than species at low (warm) latitudes. This pattern is purported to be due to the [...]
Sexual dichromatism in agile frogs: sex-reversed males closely resemble typical male coloration
Published: 2026-05-28
Subjects: Ecology and Evolutionary Biology
In species with sexual dichromatism, colouration can play an important role in intraspecific communication and affect breeding success. Communication by visual signals during the breeding season has been increasingly recognized to occur in anuran amphibians. However, most studies of sexual dichromatism have focused on consistent differences between males and females, and species with varying [...]
From Individuals to Networks: The Role of Variation in Plant-Pollinator Communities' Responses to Global Change
Published: 2026-05-28
Subjects: Biology, Ecology and Evolutionary Biology, Integrative Biology, Life Sciences
1. Plant–pollinator communities are critical for biodiversity, ecosystem function, and human well-being. Yet our ability to predict divergent species responses to environmental change, the risk of abrupt collapse, or the potential for recovery in plant-pollinator systems remains limited. 2. Here, we argue that individual variation within species may play a critical but underappreciated role in [...]
“Ecological and Conservation Governance Condition Analysis Using Ecological Diversity Indices: A Study of Bhawal National Park”
Published: 2026-05-25
Subjects: Ecology and Evolutionary Biology, Environmental Education, Environmental Health and Protection, Environmental Indicators and Impact Assessment, Forest Biology, Forest Management, Other Forestry and Forest Sciences
Abstract: Once a historical biodiversity hotspot, the Bhawal National Park (BNP) in Bangladesh faces severe threats to its ecological integrity, despite its protected status. Located approximately 40 km north of Dhaka, the park’s original coppice Sal forest (Shorea robusta) ecosystem is now fragmented and degraded due to illegal deforestation and encroachment, leading to a drastic decline in [...]
Biorestorer: A Framework for Synthetic Succession with a Qualitative System Level Illustration
Published: 2026-05-22
Subjects: Civil and Environmental Engineering, Ecology and Evolutionary Biology
Ecosystem restoration in severely degraded or soil-absent environments requires approaches that operate independently of natural soils and make effective use of locally available resources. The Biorestorer platform introduces synthetic succession as a systems-based eco-engineering framework structured in sequential functional phases and aligned with in situ resource utilization (ISRU) principles, [...]
When dangerous predators are ignored: antipredator responses in temporary-pond amphibians
Published: 2026-05-22
Subjects: Ecology and Evolutionary Biology, Life Sciences
Antipredator behaviour is recognised as a key factor of reintroduction success, yet it remains poorly considered in conservation practice. Despite their conservation relevance, little is known about antipredator behaviour in Pelobatidae tadpoles, among which the endangered Italian lineage of common spadefoot toad Pelobates fuscus has been the target of several captive breeding and reintroduction [...]
Collembola eco-morphological indices (EMI) and Soil Biological Quality Index (QBS-c): a review and practical guidelines for soil health assessment
Published: 2026-05-20
Subjects: Biodiversity, Ecology and Evolutionary Biology
Soil health assessments remain dominated by physicochemical indicators, largely due to limited functional understanding and a lack of practical tools for quantifying soil biodiversity in applied contexts. However, many soil functions are fundamentally driven by biotic components, highlighting the need for robust biological indicators. The Soil Biological Quality indices, QBS-ar and QBS-c (Parisi, [...]
Fishers’ local knowledge strengthens seagrass restoration planning
Published: 2026-05-20
Subjects: Biodiversity, Ecology and Evolutionary Biology, Marine Biology, Natural Resources and Conservation, Natural Resources Management and Policy, Social and Behavioral Sciences
Seagrass restoration is increasingly guided by habitat suitability models, yet restoration outcomes depend on more than biophysical suitability alone. In coastal social-ecological systems, fishers and anglers hold fine-scale, time-integrated knowledge of habitat condition, human use, and local constraints that are rarely incorporated at the outset of restoration planning. Here, we tested whether [...]
Colossal Disinformation
Published: 2026-05-20
Subjects: Biology, Ecology and Evolutionary Biology, Life Sciences
I have a position: de-extinction is almost impossible, however, deëxtinction, a word invented by the world’s first “de-extinction” company Colossal Laboratories & Bioscience’s can be (Max 2025); hereafter Colossal, which they define as the creation of genetically modified organisms (GMOs) that appear and act like extinct species, is possible. It’s branding, not science. I and other critics of [...]
A Protocol for Standardizing Measurements and Enabling Global Harmonization of Herbarium Leaf Reflectance Spectra
Published: 2026-05-20
Subjects: Biochemistry, Biophysics, and Structural Biology, Biodiversity, Bioinformatics, Botany, Ecology and Evolutionary Biology, Life Sciences, Other Ecology and Evolutionary Biology, Research Methods in Life Sciences
Reflectance spectroscopy offers a powerful approach to integrate high-throughput phenotypic data from herbarium specimens into the digital landscapes of ecology, evolution, and systematics. Because inconsistencies in instrumentation and measurement practices can increase noise and limit dataset compatibility, the International Herbarium Spectral Digitization Working Group (IHerbSpec) has [...]
Oak masting remains stable despite climate warming
Published: 2026-05-20
Subjects: Ecology and Evolutionary Biology, Life Sciences
Climate warming alters tree reproduction, but long-term tests of how it affects the large, synchronised interannual fluctuations in seed production known as masting remain rare. Theory predicts that climate-driven declines in masting should be most likely when species combine high reproductive sensitivity to weather cues with rapid climatic change during the corresponding cue windows. We tested [...]
Expanding the sentinel approach through multimodal integration: resolving underlying ecological processes with eDNA and computer vision
Published: 2026-05-19
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Life Sciences, Other Ecology and Evolutionary Biology, Research Methods in Life Sciences, Terrestrial and Aquatic Ecology
Sentinel approaches provide a semi-controlled method for quantifying in-field ecological interactions and processes while reducing bias and labour. They are, however, limited by difficulties ascribing taxonomic identities, behavioural context and temporal resolution to interacting agents. The integration of additional sources of data, including the analysis of DNA left behind on sentinel objects [...]
Ecoregion drives intraspecific variation of secondary chemistry in a big sagebrush common garden
Published: 2026-05-19
Subjects: Ecology and Evolutionary Biology
Phytochemistry plays an integral role in the health and survival of plants and can mediate community interactions, communication with neighboring plants, protection against disease and herbivores, and attraction of pollinators. We measured non-volatile compounds using liquid chromatography-mass spectrometry (LC-MS) and volatile compounds using gas chromatography (GC) at two different time points [...]
Removal and decomposition of fruit respond in opposite ways to canopy cover in a biodiversity experiment
Published: 2026-05-14
Subjects: Ecology and Evolutionary Biology, Entomology
Trees often produce more fruits than frugivores consume. As a result, many fruits fall to the ground, where they are either secondarily removed by vertebrates, potentially leading to seed dispersal, or they are decomposed by arthropods. Although often neglected, fallen fruits are an important component of forests and contribute to their functioning via these two distinct pathways. While fruit [...]