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Preprints

Filtering by Subject: Ecology and Evolutionary Biology

Adaptive introgression in the context of climate adaptation

Thibault Leroy, Myriam Heuertz

Published: 2025-11-27
Subjects: Ecology and Evolutionary Biology, Genetics and Genomics

As the biosphere faces accelerating environmental disruption, including climate change, and the prospect of an anthropogenically-driven mass extinction, understanding the mechanisms that enable species to adapt has become increasingly urgent. One mechanism attracting growing attention is adaptive introgression, the transfer of beneficial genetic variation between closely related species. Although [...]

Quantifying impacts of policy and practice interventions on biodiversity and climate

Mark A Bradford, Eli P. Fenichel, H. Dean Hosgood, et al.

Published: 2025-11-27
Subjects: Agriculture, Biodiversity, Ecology and Evolutionary Biology, Forest Sciences, Life Sciences

There is urgent demand for ecosystem management interventions – targeted actions through policies and practices – that meaningfully address climate change and biodiversity loss while sustaining ecosystem delivery of water, food, fibre and fuel. Rigorous quantification of intervention outcomes is required for decision makers to identify, promote and scale effective interventions. Yet [...]

Forest tree fecundity declines as climate shifts

Jessie Josepha Foest, Jakub Szymkowiak, Marcin Dyderski, et al.

Published: 2025-11-27
Subjects: Ecology and Evolutionary Biology, Terrestrial and Aquatic Ecology

Tree fecundity underpins regeneration and range tracking, yet may decline when climates exceed reproductive niches. How fecundity has changed under recent climate change remains unclear. Here,  using Polish seed harvest data including 40,530 observations for five species across 438 districts, we show a reduction in viable seed production by 32–65% across 34 years (Quercus robur, & Q. petraea [...]

Plasticity and scaling through multinucleation: a key adaptation to challenging environments

Markus Ganter, Gautam Dey, Marie Jacobovitz

Published: 2025-11-26
Subjects: Cell and Developmental Biology, Cell Biology, Ecology and Evolutionary Biology, Life Sciences

Multinucleate cells, single cells containing multiple nuclei in a shared cytoplasm, are found across the eukaryotic tree of life. Having evolved independently in fungi, plants, protists, and animals, they thrive in environments ranging from nutrient-poor deep-sea sediments to dynamic soil microhabitats and host tissues. Multinucleate organization enables spatial specialization without internal [...]

Large regional variation in global impacts of agriculture on terrestrial insects and other arthropods

Daan Scheepens, Richard Cornford, Piero Visconti, et al.

Published: 2025-11-26
Subjects: Agriculture, Biodiversity, Ecology and Evolutionary Biology

Many insects and other arthropods are reported to be in rapid decline worldwide, mainly driven by changes in land use and climate. At the same time, arthropods provide many important services that benefit agriculture, and thus their losses may pose risks to food security. Although biodiversity responses vary between global realms, this spatial heterogeneity is not well-understood and is rarely [...]

A Community-Trait-Phylogenetic Framework: Ecological and Evolutionary Integration for Soil Microarthropod Assembly

Ting-Wen Chen

Published: 2025-11-26
Subjects: Biodiversity, Ecology and Evolutionary Biology, Life Sciences

Why does a single square meter of forest soil harbour thousands of animal species? Fifty years after Jonathan M. Anderson raised this question, soil ecology still struggles with a fragmented view on the coexistence of species. Researchers often study taxonomy, functional traits, and phylogeny in isolation. Each approach adds insight but leaves gaps in the picture of soil biodiversity.  In this [...]

A novel semantic theory of the assembly rules of interaction networks

Marco A. R. Mello, Sharlene Santana, Pierre-Michel Forget, et al.

Published: 2025-11-26
Subjects: Ecology and Evolutionary Biology

In the web of life, every interaction between species tells a story of cooperation, conflict, or chance. For centuries, ecologists have charted these stories to better understand phenomena such as pollination and disease. We have been using the lens of network science to distill them into topological patterns such as nestedness or modularity. Yet, like in the old buddhist parable of the blind [...]

Humanity’s redistribution of global biomass flows

Tomas Roslin, Jason Tylianakis

Published: 2025-11-18
Subjects: Ecology and Evolutionary Biology, Life Sciences

The biosphere is connected by flows of organic material (biomass), through biogenic (e.g., animal migration) or anthropogenic pathways (e.g., trade). We argue that humans have drastically altered Earth’s biomass flows by disrupting animal movement, directly transporting biomass and creating novel biotic pathways. In 2023, transnational anthropogenic transport of biomass through trade far exceeded [...]

Response to “Radiolarian evolution: Analytical challenges in estimating the diversity and origin of Nature’s stars”

Miguel Mendez Sandin, Johan Renaudie, Noritoshi Suzuki, et al.

Published: 2025-11-14
Subjects: Biodiversity, Ecology and Evolutionary Biology, Environmental Microbiology and Microbial Ecology Life Sciences, Genetics and Genomics, Marine Biology

We appreciate Daniel Lahr’s concern (Lahr, 2025) on the interpretation and conclusions of our study “Extant diversity, biogeography, and evolutionary history of Radiolaria” (Sandin et al., 2025). Given that some of these comments were already addressed in our original study following reviewers comments and that such issues are well known in the molecular diversity and evolution fields we [...]

Dispersion tests in generalized linear mixed-effects models: a methods comparison and practical guide for ecologists

Melina de Souza Leite, Daniel Rettelbach, Florian Hartig

Published: 2025-11-14
Subjects: Applied Statistics, Ecology and Evolutionary Biology, Statistical Models

Underdispersion and overdispersion are common issues when analysing ecological data with generalized linear (mixed) models (GLMs/GLMMs). Overdispersion, the phenomenon where observations spread wider than expected by the fitted model, usually leads to anti-conservative p-values and, thus, to inflated type I error. In contrast, underdispersion, a narrower spread of the data than expected, causes [...]

Ecological, demographic and social factors shape helping decisions at different spatial scales in a facultative cooperative breeder

Jennifer Morinay, Ben J Hatchwell

Published: 2025-11-13
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Life Sciences

The fitness consequences of cooperative breeding are increasingly well understood, but the ecological and demographic factors driving helping remain contentious. Comparative and single-species studies have identified factors that promote the evolution of helping, but analyses typically test single hypotheses so the relative importance of different factors, and the spatial scale of their [...]

India’s dog crisis warrants governance reimagination, less animal management

Nishant Kumar, Tim Coulson

Published: 2025-11-13
Subjects: Behavior and Ethology, Biodiversity, Ecology and Evolutionary Biology, Life Sciences, Population Biology, Social and Behavioral Sciences

The Indian Supreme Court’s 2025 mandate to relocate millions of dogs exposed policy instability in cities where ecological realities, cultural practices, and institutional fragmentation collide. The crisis is less about animals and more about how urban governance fails to reconcile competing priorities, underscoring the collapse of the Indian coexistence model.

Beta-Diversity Beyond Bias: A Scalable Framework for Reliable Diversity Analysis from Citizen Science Data

Rahil Jasminkumar Amin, Jessie C Buettel, Leon A. Barmuta, et al.

Published: 2025-11-13
Subjects: Biodiversity, Ecology and Evolutionary Biology

Citizen science data offer unprecedented spatial and temporal coverage for biodiversity research, yet sampling biases compromise their reliability for β-diversity analyses. We introduce a comprehensive framework to address these challenges, integrating space–time scaling, quality thresholds, and multiple partitioning approaches to enhance detection of ecological signals. Applying our framework to [...]

Interacting disturbances reshape bird assemblages via divergent community trajectories across southeastern Australia

Rahil Jasminkumar Amin, Jessie C Buettel, Barry W. Brook

Published: 2025-11-12
Subjects: Biodiversity, Ecology and Evolutionary Biology

Species counts can remain stable even as ecological communities collapse. This paradox exposes a critical blind spot in biodiversity monitoring: richness metrics miss the compositional upheaval that defines modern ecological change. As human pressures intensify, specialists decline and generalists proliferate, creating numerically similar but functionally different communities. Using three [...]

The Queer & Trans Field Safety Assessment: a tool for protecting minoritized field scientists

Amelia Hope Reynolds, Evan Ho, Ronnie Steinitz, et al.

Published: 2025-11-12
Subjects: Ecology and Evolutionary Biology, Life Sciences

Ecological fieldwork poses heightened risks for LGBTQIA+ scientists due to inadequate safety protocols and identity-based vulnerabilities. Best practices to improve safety for queer field researchers exist, yet over 50% of LGBTQIA+ field scientists report feeling unsupported, with structural and cultural barriers unaddressed. Our team of 15 researchers from the University of California developed [...]

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