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Preprints

Filtering by Subject: Life Sciences

Functional or Dysfunctional? A Refreshed Perspective on Functional Feeding Groups

Finlay Ryder, Fredric M. Windsor

Published: 2025-12-11
Subjects: Ecology and Evolutionary Biology, Life Sciences, Terrestrial and Aquatic Ecology

Functional feeding groups (FFGs), introduced in the 1970s, provided a framework for understanding freshwater ecosystems by classifying macroinvertebrates according to feeding mode and diet. Since then, the FFG approach has been widely applied and has significantly advanced our knowledge of ecosystem processes and functions. However, the framework represents a simplified view of complex trophic [...]

Spores of arbuscular mycorrhizal fungi inhabiting inside mossballs of Rigodium implexum

Roberto Godoy, Paula Aguilera, Javier Retamal, et al.

Published: 2025-12-11
Subjects: Life Sciences

The spores of four species of arbuscular mycorrhizal fungi (Phylum: Glomeromycota): Acaulospora laevis, Acaulospora sieverdingii, Ambispora gerdemannii, and Dominikia aurea, were found inhabiting inside mosballs formed by Rigodium implexum at two forest sites in southern Chile. These species were identified through morphological keys. R. implexum mossballs are usually 10-20 cm in diameter, [...]

How pesticide exposure and effects match with the intention of European pesticide regulation – a mini review

Ralf B. Schäfer, Juliane Filser, Matthias Liess, et al.

Published: 2025-12-11
Subjects: Life Sciences

Pesticides are widely used in European agriculture, requiring robust prospective risk assessment of their environmental exposure and effects on non-target species to safeguard biodiversity. We conducted a high-level evaluation to test whether: 1) measured environmental exposure concentrations remain below those established by risk assessment and 2) these concentrations prevent population- or [...]

Behavioural repertoire and the onset of precocious sexual behaviours in juvenile ruffs (Calidris pugnax)

Veronika A. Rohr-Bender, Udit Nair, Lina M. Giraldo-Deck, et al.

Published: 2025-12-10
Subjects: Animal Studies, Behavior and Ethology, Biology, Ecology and Evolutionary Biology, Life Sciences

Adult phenotypes are shaped by developmental processes during early life. This also applies to reproduction and dominance-related behaviours before maturation, called precocious sexual behaviours. However, beyond largely anecdotal reports, the onset of such behaviours is rarely studied. Here we document the development of precocious sexual behaviours in the ruff Calidris pugnax, a lekking [...]

The Origins of Human Cooperation Through Intergroup Relations in Our Closest Living Relatives

Luke Daniel Bakic-Pawlak, Stuart Wigby

Published: 2025-12-10
Subjects: Life Sciences, Social and Behavioral Sciences

Cooperation is the foundation of human society, yet our understanding of its evolutionary origins and underlying mechanisms remains limited. This review draws on intergroup encounters in our closest evolutionary relatives, chimpanzees (Pan troglodytes) and bonobos (Pan paniscus), to better understand the evolutionary pressures and processes that shaped human cooperation. In chimpanzees, [...]

PROPOSAL of A MONITORING PLAN for THE EUROPEAN EEL, ANGUILLA ANGUILLA L. 1758, IN SICILY.

ANTONINO DUCHI

Published: 2025-12-10
Subjects: Life Sciences

We outline a monitoring plan for European eel that has been proposed in 2021, as part of of the preparatory activities for the elaboration of the Regional Eel Management Plan for Sicily (project FEAMP OP 2014/2020, MEASURE 2.49, Sicilian Regional Administration), to be included in the National Management Plan (Regulation EC 1100/2007). Sampling methods and time-frames diversified for juveniles, [...]

A Satellite-based Approach to Investigating Eutrophication in Lakes Receiving Wastewater Treatment Effluent: A Case Study of Lake Windermere

Sabbir Delowar, Mark Scrimshaw

Published: 2025-12-10
Subjects: Life Sciences, Physical Sciences and Mathematics

Excessive phosphorus from the sewer systems in the UK is a major contributor to eutrophication in freshwater bodies. Chlorophyll-a levels are widely used as a reliable indicator of phosphorus driven eutrophication, and satellite remote sensing provides an effective means of monitoring these dynamics at high spatial and temporal resolution. This study evaluated the utility of Sentinel-2 imagery in [...]

Acorn availability reduces agricultural damage by ungulates

Maria Bogdańska, Valentin Journe, Michał Bogdziewicz

Published: 2025-12-10
Subjects: Ecology and Evolutionary Biology, Life Sciences

Human-wildlife conflicts, particularly the damage to agricultural crops caused by ungulates, pose significant ecological and economic challenges. Understanding the role of natural food availability in driving these conflicts is important for developing effective management strategies. We investigated how the pulsed availability of forest tree seeds, i.e., mast seeding, influences the extent of [...]

The Plant Communities of Oman’s Central Coastline: A Baseline Ecological Assessment amid Rising Threats

Joshua Robert Taylor, Rebecca A Senior, Laila Said Al Harthy, et al.

Published: 2025-12-10
Subjects: Biodiversity, Botany, Desert Ecology, Life Sciences, Plant Sciences

In recent decades, the botanical knowledge within the Sultanate of Oman has advanced considerably. However, the coastal vegetation is comparatively understudied with much of the country’s extensive coastline still undocumented. This is despite Oman’s coast being faced with a plethora of threats including development, overgrazing and the invasive species Neltuma juliflora. This study presents the [...]

Microbial contributions to host life history tradeoffs

Lauren Petrullo, Greg Albery, Aura Raulo, et al.

Published: 2025-12-09
Subjects: Life Sciences

All organisms must allocate finite resources among growth, maintenance, and reproduction, generating trade-offs that constrain adaptation. Here, we argue that host microbiomes are dynamic resource engines capable of reallocating and generating energy and resources for their hosts. In doing so, they may recalibrate the tradeoffs fundamental to life history evolution. 

Quantifying Three Decades of Artisanal and Small-scale Gold Mining Frontiers in the Guiana Shield (1995–2024)

Elmontaserbellah Ammar, Sean J Glynn, Kerry Anne Kansinally, et al.

Published: 2025-12-09
Subjects: Artificial Intelligence and Robotics, Biodiversity, Earth Sciences, Ecology and Evolutionary Biology, Environmental Monitoring, Environmental Sciences, Geography, Life Sciences, Natural Resources and Conservation, Remote Sensing, Sustainability, Terrestrial and Aquatic Ecology

Artisanal and Small-scale Gold Mining (ASGM) is a leading driver of tropical deforestation and forest degradation, yet its spatial and temporal dynamics remain largely underexplored. Here, we present a pan-regional, annual time-series analysis of ASGM expansion across rainforests of the Guiana Shield (Guyana, Suriname, and French Guiana) from 1995 to 2024. Using Landsat imagery, we trained a deep [...]

A chronosequence of human remains on soil microbial populations

Jason Reynolds, Natasha Robinson, Rani Carroll, et al.

Published: 2025-12-08
Subjects: Life Sciences, Physical Sciences and Mathematics

Surficial human decomposition produces substantial and measurable shifts in soil chemistry and microbial composition. This decade-long investigation examined temporal changes beneath surface deposited human remains and identified strong microbial and chemical responses in the first twelve to twenty four months, including decreased microbial diversity, elevated soil nutrients and sustained [...]

Gene expression and structural differences underpinning black and white colouration in spiders

Fabian C. Salgado-Roa, Devi Stuart-Fox, Laura Ospina-Rozo, et al.

Published: 2025-12-05
Subjects: Ecology and Evolutionary Biology, Entomology, Life Sciences

The presence of multiple colour variants within a single population, known as colour polymorphism, is a striking example of intraspecific phenotypic variation and a model for studying evolutionary processes. These processes depend on the underlying mechanisms such as the genes and pigments involved; but we currently lack such information for non-model groups such as arachnids. We examined the [...]

Butterfly abundances but not patch occupancy buffered by vegetation heterogeneity during climatic extremes

Natalie E. van Dis, Mirkka M. Jones, Pekka Niittynen, et al.

Published: 2025-12-04
Subjects: Ecology and Evolutionary Biology, Life Sciences

As extreme climatic events are becoming more frequent and intense, we need to understand what mechanisms could buffer populations from climate change impacts to ensure their persistence. Fragmented populations may be especially vulnerable to climate change, as exemplified by species with metapopulation dynamics, whose persistence is determined by a precarious balance between frequent local [...]

Identification of the Cichlid Fishes of Lake Malawi/Nyasa Part 3: Rhamphochromina and others.

George Francis Turner, Martin J Genner

Published: 2025-12-04
Subjects: Life Sciences

With an estimated 800-1000 species, the cichlid fishes of Lake Malawi represent the largest known adaptive radiation of vertebrates from common ancestry in a limited geographical area, in this case a single lake. Although they represent a major system for the study of evolution, and have considerable local economic value, the presence of so many closely-related and poorly known species presents [...]

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