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Preprints

Filtering by Subject: Life Sciences

Habitat amount control is necessary but not sufficient to resolve the fragmentation debate

Juan Andrés Martínez Lanfranco

Published: 2026-03-24
Subjects: Life Sciences

Opposing conclusions from the same global multi-taxa dataset have intensified debate over whether fragmentation effects can be inferred independently of habitat amount in observational landscape studies. Gonçalves-Souza et al. (2025) reported lower local- and landscape-scale diversity in fragmented landscapes, whereas Fahrig et al. (2026) reanalysed the same dataset with continuous, scale-matched [...]

Microbial Inoculants for Soil Restoration: A Practical Framework for Risk-Governed Stewardship

Anna Edlund, Gwyn Beattie, Joana Falcao Salles, et al.

Published: 2026-03-24
Subjects: Life Sciences

Global soil degradation and increasing reliance on chemical inputs threaten agricultural sustainability, driving interest in microbial inoculants as tools for soil restoration. These biological products have the potential to enhance nutrient cycling, improve soil structure, and support plant resilience, but their environmental release raises important safety and stewardship considerations. Here, [...]

Coexistence of phenotypic plasticity and habitat use in natural populations

Samantha Clare Patrick, Julien G. A. Martin, anne charmantier, et al.

Published: 2026-03-23
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Evolution, Life Sciences

When studying how individuals adapt to environmental changes, the environment is traditionally viewed as a passive backdrop, with individuals modifying their phenotype in response to environmental conditions (i.e., phenotypic plasticity). However, this perspective overlooks the active role of habitat choice in mediating individual responses to environmental changes. In this paper, we argue for [...]

Direct and biodiversity-mediated effects of climate on grassland productivity across the Alps

Pierre Gauzere, Maria Hällfors, Jussi Mäkinen, et al.

Published: 2026-03-23
Subjects: Ecology and Evolutionary Biology, Life Sciences

Understanding how climate shapes ecosystem productivity through both energetic constraints and biodiversity‑mediated pathways remains a major challenge in global change ecology, particularly in mountain grasslands where rapid warming and strong environmental gradients interact. Here, we disentangle and map direct climatic controls on productivity from indirect effects mediated by canopy [...]

High Data Quality Enhances Microplastic Toxicity Prediction

Ana L. Antonio Vital, Scott Coffin, Andrea Bonisoli-Alquati, et al.

Published: 2026-03-23
Subjects: Life Sciences

Unlike chemicals, microplastics (MPs) lack standardized identifiers, limiting the applicability of traditional predictive ecotoxicology methods such as quantitative structure-activity relationship (QSAR) models. This study aimed to predict MP toxicity using MP properties, MP concentration, organismal traits, endpoints, and experimental design, and to evaluate how data pre-processing, dataset [...]

Missing the mammals for the trees: comparative biogeography of southern Appalachian sky island biodiversity

Kimberly July Cook, David Webster, Juliet Stowe, et al.

Published: 2026-03-20
Subjects: Life Sciences

Sky islands are patches of relic ecosystems on mountaintops, often forest types, that were previously connected in the geological past and are now patchily distributed. Whether a particular sky island forest serves as an umbrella for conserving sky island fauna with a similar patchy distribution is an open question. To address this, we systematically delineate sky islands and identify [...]

Towards a better understanding of adaptation: Problem description, partial solutions, and recommendations

Pim Edelaar, Niels J Dingemanse, Samantha C. Patrick, et al.

Published: 2026-03-20
Subjects: Animal Experimentation and Research, Animal Studies, Behavior and Ethology, Biodiversity, Developmental Biology, Evolution, Human Ecology, Integrative Biology, Life Sciences, Other Ecology and Evolutionary Biology, Other Genetics and Genomics, Other Public Affairs, Public Policy and Public Administration, Population Biology, Science and Technology Studies

This paper is the product of an international workshop aiming to make progress in our general understanding of adaptation. We met from 5-7 February 2025 in Hannover (Germany), funded by the foundation “Volkswagen Stiftung”. For our group of theoretical and empirical biologists, social scientists, and philosophers of science we set up a program to facilitate communication and collaboration between [...]

(R)evolution Stings Back: Rethinking Strategies for Conserving Local Biodiversity of the Western Honey Bee (Apis mellifera)

Michał Robert Kolasa, Bartłomiej Molasy, Andrzej Oleksa

Published: 2026-03-19
Subjects: Life Sciences

The western honey bee (Apis mellifera) occupies an unusual position between domesticated livestock and wild organisms, creating persistent ambiguity in conservation policy. Most current conservation programmes prioritise controlled breeding, phenotypic stability, and lineage integrity, implicitly treating honey bees as populations dependent on continuous human management. While effective at [...]

iDeer: A decision-support tool for managing deer alongside woodland creation

Amy Gresham, Matthew Grainger, Matt Guy, et al.

Published: 2026-03-19
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Forest Management, Life Sciences, Terrestrial and Aquatic Ecology

Increasing deer (Cervidae) densities driven by land-use change and climate warming represent a growing challenge to the establishment and management of woodlands across temperate biomes. Targeting deer management is challenging without spatially explicit information on potential impact risks under alternative management scenarios. Here we present the iDeer Tool [...]

Permissible Spite: Kin Selection, Demography, and the Inverse Hamiltonian Equation

Olga Semenova

Published: 2026-03-19
Subjects: Biology, Life Sciences, Social and Behavioral Sciences, Systems Biology

This article revisits Hamilton’s rule by proposing an inverted formulation to evaluate the evolutionary permissibility of spiteful behavior within kin-based populations. We formalize a reverse Hamiltonian equation and apply replicator dynamics to investigate the demographic and genetic conditions under which within group aggression may become evolutionarily stable. The model shows that in [...]

Reintroducing a nationally extinct predator, the forest caterpillar hunter (Calosoma sycophanta), for biocontrol of the invasive oak processionary (Thaumetopoea processionea) in Britain: considerations, benefits and risks

Kyle Alexander Miller, Jordan Patrick Cuff, Katiana Saleiko, et al.

Published: 2026-03-18
Subjects: Life Sciences

1. Controlling invasive species remains one of the greatest challenges of the 21st century. Sustainably managing invasive pests like the oak processionary moth (Thaumetopoea processionea; OPM), which lacks natural enemies in Britain, may require the introduction, or reintroduction, of suitable biocontrol agents. 2. The forest caterpillar hunter (Calosoma sycophanta; FCH) is thought to have been [...]

Historical mating systems and the origin of sexual ornament

Andrew Crompton

Published: 2026-03-18
Subjects: Life Sciences

Ornamental traits are commonly interpreted as products of runaway sexual selection or costly signals of male quality, but many ornamental traits appear to be functionally redundant, and in birds especially, multiple ornaments coexist without clear links to condition or survival. This new proposal explains the evolution of ornament through sexual selection. based on looking for historic [...]

The Finite Element method to aid modelling of complex ecological systems

Klementyna A. Gawecka, James M. Bullock

Published: 2026-03-18
Subjects: Ecology and Evolutionary Biology, Life Sciences

Predicting how biodiversity responds to environmental change and management interventions remains a major challenge in ecology. Ecological systems are shaped by the interplay of demographic processes, species interactions, dispersal, and spatial heterogeneity across landscapes. Yet, many existing modelling approaches face a trade-off between spatial and ecological complexity, which limits their [...]

The scent of survival in a warming world: how monoterpenes drive thermal adaptation in thyme

Andreas Havbro Faber, John D Thompson, Perrine Gauthier, et al.

Published: 2026-03-18
Subjects: Comparative and Evolutionary Physiology, Ecology and Evolutionary Biology, Evolution, Life Sciences, Other Ecology and Evolutionary Biology, Other Plant Sciences, Physiology, Plant Biology, Plant Sciences, Population Biology

1 Monoterpenes are key plant secondary metabolites with well known defensive and ecological functions, yet their role in abiotic stress tolerance remains poorly understood. In many Mediterranean plants, monoterpene composition varies markedly within and among species and is associated with climatic gradients, suggesting that these compounds may mediate plant responses to extreme heat. 2 We [...]

Adaptive strategies for biodiversity monitoring integrating Indigenous ecological calendars and community science data

Orlando Acevedo-Charry, Gloria Amparo Rivera Velasco, Walter Gabriel (Siorú) Estrada, et al.

Published: 2026-03-18
Subjects: Life Sciences

Integrating Indigenous and Western ecological knowledge can strengthen understanding of phenological patterns, yet this integration is often constrained by epistemological differences, power asymmetries, and histories of exclusion. We evaluated the potential of the Two-Eye Seeing guiding principle, which integrates the strengths of both knowledge systems, in ongoing biocultural conservation [...]

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