Preprints
Filtering by Subject: Animal Experimentation and Research
Genetic variance and phenotypic selection on pathogen-linked oviposition choice in Drosophila
Published: 2026-03-23
Subjects: Animal Experimentation and Research, Behavior and Ethology, Entomology, Evolution, Genetics, Integrative Biology, Research Methods in Life Sciences, Zoology
Pathogen-avoidance behaviour is assumed to be adaptive, yet its phenotypic variability and genetic heritability are rarely quantified. In species lacking post-oviposition care, avoiding potentially infectious egg-laying substrates would improve offspring survival and should therefore be under strong selection. We used two-choice oviposition assays to quantify the phenotypic and genetic variance [...]
Insect oviposition as a simple system to investigate the ecology and evolution of pathogen avoidance behaviour
Published: 2026-03-20
Subjects: Animal Experimentation and Research, Behavior and Ethology, Biology, Entomology, Evolution, Immunology and Infectious Disease, Integrative Biology, Laboratory and Basic Science Research Life Sciences, Research Methods in Life Sciences, Zoology
Behavioural avoidance of pathogens and parasites is a ubiquitous first line of defence, yet we lack tractable systems that connect cue detection to fitness consequences, population transmission, and coevolution. We propose insect oviposition as a model that yields general principles for avoidance across taxa. Oviposition decisions fix offspring exposure, they are governed by well‑mapped sensory [...]
Towards a better understanding of adaptation: Problem description, partial solutions, and recommendations
Published: 2026-03-20
Subjects: Animal Experimentation and Research, Animal Studies, Behavior and Ethology, Biodiversity, Developmental Biology, Evolution, Human Ecology, Integrative Biology, Life Sciences, Other Ecology and Evolutionary Biology, Other Genetics and Genomics, Other Public Affairs, Public Policy and Public Administration, Population Biology, Science and Technology Studies
This paper is the product of an international workshop aiming to make progress in our general understanding of adaptation. We met from 5-7 February 2025 in Hannover (Germany), funded by the foundation “Volkswagen Stiftung”. For our group of theoretical and empirical biologists, social scientists, and philosophers of science we set up a program to facilitate communication and collaboration between [...]
Composite virulence: useful metric or conceptual trap?
Published: 2026-02-20
Subjects: Animal Diseases, Animal Experimentation and Research, Animal Sciences, Biodiversity, Biology, Ecology and Evolutionary Biology, Evolution, Immunity, Immunology and Infectious Disease, Immunology of Infectious Disease, Immunopathology, Life Sciences, Medical Microbiology, Microbiology, Other Ecology and Evolutionary Biology, Other Immunology and Infectious Disease, Parasitic Diseases, Parasitology, Pathogenic Microbiology, Plant Pathology, Research Methods in Life Sciences, Zoology
Virulence, the harm an infection causes to its host, is a cornerstone concept in ecology and evolution, yet it remains difficult to quantify because infection impact is multidimensional, dynamic, and context-dependent. Infections can reduce host performance through multiple, partially redundant routes (including mortality, fecundity loss, behavioural impairment, and physiological disruption), [...]
The myth of the metabolic baseline: how sleep-wake cycles undermine a foundational assumption in organismal biology
Published: 2025-08-28
Subjects: Animal Experimentation and Research, Biochemistry, Biophysics, and Structural Biology, Biology, Comparative and Evolutionary Physiology, Life Sciences, Physiology, Research Methods in Life Sciences, Zoology
Basal and standard metabolic rates (BMR and SMR) are cornerstones of physiological ecology and are assumed to be relatively fixed intrinsic properties of organisms that represent the minimum energy required to sustain life. However, this assumption is conceptually flawed. Many core maintenance processes underlying SMR are temporally partitioned across sleep and wakefulness and are not [...]
Transferable approaches to CRISPR-Cas9 induced genome editing in non-model insects: a brief guide
Published: 2025-02-27
Subjects: Animal Experimentation and Research, Animal Sciences, Entomology, Genetics, Life Sciences, Molecular Genetics, Other Genetics and Genomics, Zoology
Despite the large variety of insect species with divergent morphological, developmental and physiological features questions on gene function could for a long time only be addressed in few model species. The adoption of the bacterial CRISPR-Cas system for genome editing in eukaryotic cells widened the scope of the field of functional genetics: for the first time the creation of heritable genetic [...]
Lights tuned to the avian eye result in early detection and escape from an approaching aircraft
Published: 2025-01-08
Subjects: Animal Experimentation and Research, Animal Sciences, Animal Studies, Behavioral Neurobiology, Social and Behavioral Sciences
Collisions between birds and aircraft are a global problem. We identified different behavioral parameters affecting the probability of escape to an approaching aircraft, which is a function of the probability that the animal initiates an escape response (probability of reaction) and the probability of having enough time to escape (probability of sufficient time). Lights of high chromatic contrast [...]
Reporting guidelines for terrestrial respirometry: Building openness, transparency of metabolic and evaporative water loss data
Published: 2024-02-20
Subjects: Animal Experimentation and Research, Comparative and Evolutionary Physiology, Integrative Biology, Life Sciences
Respirometry is an important tool for understanding whole-animal energy and water balance in relation to the environment. Consequently, the growing number of studies using respirometry over the last decade warrants reliable reporting and data sharing for effective dissemination and research synthesis. We provide a checklist guideline on five key sections to facilitate the transparency, [...]
Switching to bioplastics may exacerbate ingestion of lost and discarded fishing gear by marine invertebrates
Published: 2024-01-09
Subjects: Animal Experimentation and Research, Biology, Environmental Health Life Sciences, Life Sciences, Marine Biology, Natural Resources and Conservation, Sustainability
Bioplastics are argued to be more environmentally sustainable than conventional plastics. Yet, little is known about how bioplastics degrade in marine environments or their likelihood of being ingested by animals. We measured changes in the weight of biodegradable, semi-biodegradable, and non-biodegradable fishing gears (soft plastic lures, SPLs) in or out of seawater over 14 days. We then [...]
Quantifying clearance rates of restored shellfish reefs using modular baskets
Published: 2023-12-08
Subjects: Animal Experimentation and Research, Aquaculture and Fisheries Life Sciences, Biology, Ecology and Evolutionary Biology, Environmental Indicators and Impact Assessment, Environmental Monitoring, Environmental Sciences, Life Sciences, Marine Biology, Oceanography and Atmospheric Sciences and Meteorology, Research Methods in Life Sciences, Terrestrial and Aquatic Ecology, Water Resource Management, Zoology
Shellfish reefs are among the most degraded ecosystems globally, prompting substantial efforts to restore them. While biodiversity gains of restored reefs are well documented, other ecosystem services such as water filtration remain poorly quantified. We present a novel way of measuring water filtration by restored reefs using modular restoration structures called Robust Oyster Baskets (ROB 400). [...]
ForageFeeder: a low-cost open source feeder for randomly distributing food
Published: 2022-12-30
Subjects: Animal Experimentation and Research, Life Sciences, Research Methods in Life Sciences
Automated feeders have long fed mice, livestock, and poultry, but are incapable of feeding zoo animals such as gorillas. Gorilla food, consisting of cut vegetables and fruits, is too large to be dispensed by automated feeders. Instead gorillas are manually at a set time and location, which decreases the exercise and enrichment that accompanies their natural foraging. We designed and built [...]
Repeatability of performance within and across contexts measuring behavioral flexibility
Published: 2022-08-10
Subjects: Animal Experimentation and Research, Behavior and Ethology, Ecology and Evolutionary Biology, Life Sciences, Research Methods in Life Sciences
Research into animal cognitive abilities is increasing quickly and often uses methods where behavioral performance on a task is assumed to represent variation in the underlying cognitive trait. However, because these methods rely on behavioral responses as a proxy for cognitive ability, it is important to validate that the task structure does, in fact, target the cognitive trait of interest [...]
Intergenerational effects of overfeeding on aversive learning in zebrafish (Danio rerio)
Published: 2022-02-07
Subjects: Animal Experimentation and Research, Behavior and Ethology, Ecology and Evolutionary Biology, Life Sciences, Research Methods in Life Sciences
The obesity epidemic is among the most serious and rapidly growing public health challenges of the 21st century. This rapid increase is concerning as obesity appears to negatively impact cognition and behaviour. Furthermore, some studies suggest that this negative effect could be carried across generations from both mothers and fathers although evidence is not consistent. Here, we attempt to [...]
The effects of an obesogenic diet on behaviour and cognition in zebrafish (Danio rerio): traits average, variability, repeatability, and behavioural syndromes
Published: 2022-02-07
Subjects: Animal Experimentation and Research, Behavior and Ethology, Ecology and Evolutionary Biology, Life Sciences, Research Methods in Life Sciences
The obesity epidemic, largely driven by the accessibility of ultra-processed high-energy foods, is one of the most pressing public health challenges of the 21st century. Consequently, there is increasing concern about the impacts of diet-induced obesity on behaviour and cognition. While research on this matter continues, to date, no study has explicitly investigated the effect of obesogenic diet [...]
An efficient new assay for measuring zebrafish anxiety: tall tanks that better characterize between-individual differences
Published: 2020-11-18
Subjects: Animal Experimentation and Research, Life Sciences, Research Methods in Life Sciences
Zebrafish (Danio rerio) are increasingly being used to model anxiety. A common behavioral assay employed for assessing anxiety-like behaviors in zebrafish is the “novel tank test”. We hypothesized that using deeper tanks in this test would result in greater between-individual variation in behavioral responses and a more ‘repeatable’ assay. After mapping the literature and identifying common [...]