Preprints
Filtering by Subject: Life Sciences
Forest composition and diversity buffer microclimates and enhance productivity
Published: 2025-06-17
Subjects: Life Sciences
Trees can buffer forest ecosystems against climate extremes, creating microenvironments that support diverse plant communities. We asked how diverse tree communities could influence the above- and below-ground moisture environment, thereby linking tree diversity and ecosystem function. To address this question, we integrated remotely sensed forest structure, growth inventories, microclimate [...]
Enhancing motivation to learn marine ecology and increase ocean literacy through Virtual Reality in Higher Education
Published: 2025-06-17
Subjects: Ecology and Evolutionary Biology, Life Sciences, Marine Biology, Other Social and Behavioral Sciences, Psychology, Social and Behavioral Sciences
Virtual Reality (VR) is increasingly recognised as a tool for enhancing engagement and motivation in education. This is particularly true where access to experiential learning is limited, as is often the case in marine ecology courses. However, the effectiveness of VR for teaching and learning in higher education is poorly understood. Here, we use the Explore experience developed by The Hydrous [...]
Elevating the importance of Risk of Bias assessment for ecology and evolution
Published: 2025-06-17
Subjects: Agriculture, Animal Sciences, Biodiversity, Biology, Ecology and Evolutionary Biology, Entomology, Life Sciences, Marine Biology, Microbiology, Plant Sciences, Research Methods in Life Sciences
Systematic reviews and meta-analyses are key evidence synthesis methods informing research and policy. An assessment of the Risk of Bias (RoB) in included studies is normally considered an essential component of these. However, RoB assessment is rare in ecology and evolutionary biology (EEB), and tools from other fields are seldom adopted. To identify reasons for this limited uptake, we surveyed [...]
Comparing statistical methods for detecting weather cues of mast seeding in European beech (Fagus sylvatica) across Europe
Published: 2025-06-17
Subjects: Biology, Ecology and Evolutionary Biology, Forest Sciences, Life Sciences, Plant Sciences
Understanding the drivers of mast seeding is important for predicting reproductive dynamics in perennial plants. Here, we evaluate the performance of four statistical methods for identifying weather-associated drivers of annual seed production, i.e, weather cues: climate sensitivity profile, P-spline regression, sliding window analysis, and peak signal detection. Using long-term seed production [...]
Characterising the structural complexity across major habitats of Tenerife, Spain
Published: 2025-06-13
Subjects: Biodiversity, Ecology and Evolutionary Biology, Environmental Monitoring, Life Sciences
Understanding biodiversity changes across ecosystems requires the consideration of various biodiversity dimensions, such as habitat structural complexity – the degree of heterogeneity in the distribution of plant material in three-dimensional space. Yet, its inclusion in long-term biodiversity monitoring on oceanic islands remains limited. Terrestrial laser scanning (TLS) can be used to quantify [...]
Toxin resistance mechanisms span biological scales in the Royal Ground Snake (Colubridae: Erythrolamprus reginae)
Published: 2025-06-13
Subjects: Animal Sciences, Behavior and Ethology, Biology, Cellular and Molecular Physiology, Comparative and Evolutionary Physiology, Ecology and Evolutionary Biology, Evolution, Genomics, Integrative Biology, Life Sciences, Molecular Biology, Neuroscience and Neurobiology, Physiology, Systems and Integrative Physiology Life Sciences, Systems Biology, Zoology
Exposure to multiple toxic compounds imposes selective pressures across biological levels. There are several known toxin resistance mechanisms–such as behavioral avoidance, metabolic detoxification, and target-site insensitivity but an integrative approach to consider multiple toxins and resistance strategies. Predators of amphibians, for example, must counteract multiple chemicals secreted by [...]
Genomic data confirms that mutation cannot restore genetic diversity lost through population bottlenecks
Published: 2025-06-13
Subjects: Ecology and Evolutionary Biology, Genomics, Life Sciences, Molecular Genetics, Population Biology
Maintaining within-species genetic diversity is a critical goal of biodiversity conservation as it determines a species’ ability to adapt to environmental change. Without human intervention, isolated populations can only recover genetic diversity post-bottleneck through the accumulation of new mutations over evolutionary timescales. Using recent estimates of mutation rates from major genomic [...]
When homology fails: lessons from liver-fluke phylogenies
Published: 2025-06-12
Subjects: Life Sciences
Misaligned sequences derail evolutionary inference. Datasets from GenBank require verification of positional homology and orientation before alignment and phylogenetic analysis. Liver-fluke case studies reveal how overlooked errors skew results, underscoring the need for rigorous checks in parasitology and all molecular research.
Colorful birds face heightened extinction risk around the world
Published: 2025-06-10
Subjects: Biodiversity, Ecology and Evolutionary Biology, Environmental Studies, Life Sciences, Ornithology
Many of the functional traits that mediate extinction risk across the tree life relate indirectly to a species’ ability to persist in a changing world. Yet, there are certain traits such as coloration that directly affect human interactions with wildlife. Here, we use an existing dataset of color metrics for 4334 passerine bird species combined with global functional trait data to determine [...]
The interplay between ecological networks drives host-plasmid community dynamics
Published: 2025-06-10
Subjects: Ecology and Evolutionary Biology, Life Sciences
Plasmids drive bacterial evolution by transferring adaptive traits, such as metabolism and antimicrobial resistance, between members of microbial communities. There are trends and barriers to plasmid transmission, which likely emerge from both host-plasmid interactions (e.g. plasmid host ranges), and plasmid-plasmid interactions (e.g. plasmid incompatibility). However, while both interaction [...]
Delayed Reproduction as a Driver of Longevity
Published: 2025-06-10
Subjects: Life Sciences
Understanding the evolutionary forces that shape aging is central to both biology and medicine. While classic theories—such as Medawar’s mutation accumulation, Williams’ antagonistic pleiotropy, and Kirkwood’s disposable soma—have provided foundational insights, the population-level consequences of reproductive timing remain underexplored. Here, I propose that delayed reproduction may intensify [...]
Subterranean environments contribute to three-quarters of classified ecosystem services
Published: 2025-06-06
Subjects: Life Sciences
Beneath the Earth’s surface lies a network of interconnected caves, voids, and systems of fissures forming in rocks of sedimentary, igneous, or metamorphic origin. Though largely inaccessible to humans, this hidden realm supports and regulates services critical to ecological health and human well-being. Subterranean ecosystems are integral to major biogeochemical cycles, sustain diverse surface [...]
Withdrawn: Which phenotypic traits are under selection under warm, dry climates in black spruce?
Published: 2025-06-06
Subjects: Life Sciences
• Trees are increasingly at risk of maladaptation to their environment as climates change rapidly world-wide. Although adaptive evolution by natural selection is a key mechanism by which populations and species can avoid extinction in changing environments, we have limited information regarding the phenotypic traits under selection under warm and dry environments. We answer the following research [...]
Vaccination and immigration rates influence raccoon rabies elimination and recolonization in simulated urban-suburban landscapes
Published: 2025-06-04
Subjects: Life Sciences
The raccoon variant of the rabies virus (RRV) is managed in the eastern United States and Canada via distribution of oral rabies vaccine (ORV) baits. The goal of ORV distribution is to reach seroprevalence rates (an index of population immunity) of at least 60%, the threshold thought to eliminate RRV. Seroprevalence rates in urban areas rarely reach target levels, predictably leading to rabies [...]
Relative High Fitness and Large Genome Size May Lead to the High Diversification of Plastic Foragers
Published: 2025-06-04
Subjects: Ecology and Evolutionary Biology, Life Sciences
Explaining variation in diversification of species across the Tree of Life is an important challenge for evolutionary biologists. Growing evidence suggests that key innovations or historical contingency give rise to high diversification of species, but the genetic mechanisms through which this process may occur remain poorly investigated. Based on fitness landscapes, a high diversification is [...]