Preprints
Filtering by Subject: Entomology
Stridulation rate as a thermometer in Tettigonia viridissima: a season-long automated record and a test against archive recordings
Published: 2026-10-15
Subjects: Ecology and Evolutionary Biology, Entomology
That an insect's song rate follows its temperature has been known since Dolbear proposed reading a cricket as a thermometer in 1897. The relation is not in doubt; its functional form is. Walker (1975a) found stridulation rate approximately linear in ten species of Orchelimum and, reanalysing fifteen published datasets, found linear fits better than exponential in thirteen — but his data were [...]
Beyond the Vertebrate Model: Insights from Insect Stress Responses
Published: 2026-10-15
Subjects: Animal Sciences, Animal Studies, Behavioral Neurobiology, Behavior and Ethology, Biology, Cell and Developmental Biology, Cellular and Molecular Physiology, Cognitive Neuroscience, Comparative and Evolutionary Physiology, Ecology and Evolutionary Biology, Entomology, Evolution, Genetics, Molecular Genetics, Systems Biology, Zoology
Stress responses are essential for survival, coordinating behavioural, physiological and molecular adjustments that enable organisms to cope with challenges. Most research has focused on vertebrates, leaving invertebrate stress physiology comparatively underexplored, particularly in insects. In this review, we compare insect and vertebrate stress responses to examine what insects reveal about the [...]
Diurnal temperature fluctuations outweigh population origin in shaping thermal performance and range predictions in European Aedes albopictus
Published: 2026-09-30
Subjects: Ecology and Evolutionary Biology, Entomology, Life Sciences, Zoology
Predicting invasive ectotherm responses to climate change requires understanding how population-level variation and diurnal temperature fluctuations shape thermal performance, and whether constant-temperature measurements accurately capture performance under fluctuating conditions. Yet many models, including those used to predict mosquito distribution and disease transmission, still rely on [...]
Modeling Decomposition: Beyond a Well-Mixed World
Published: 2026-09-28
Subjects: Botany, Ecology and Evolutionary Biology, Entomology
Carbon cycle models overlook resource patchiness and networked foraging characterizing terrestrial carbon dynamics. They treat inputs as well mixed and decomposers as acting at local scales, making substrate stoichiometry, rather than spatial access, the primary constraint on decomposition. Using deadwood decomposition by cord-forming fungi and termites as examples, we illustrate how resource [...]
INSTAR: reporting items for invertebrate welfare in research
Published: 2026-09-24
Subjects: Animal Sciences, Biology, Ecology and Evolutionary Biology, Entomology, Life Sciences
Concern for the welfare of research animals has expanded rapidly across taxa, with increasing focus on invertebrates. Recent evidence for affective experience in cephalopod, decapod and insect taxa, alongside shifts in public attitudes and regulation, has raised the stakes of how invertebrates are used and reported, yet no reporting standard adequately addresses them. Here we present INSTAR [...]
Evaluating vibroacoustic sensor performance and vibration attenuation: implications for soil biodiversity sampling
Published: 2026-09-11
Subjects: Entomology, Life Sciences, Research Methods in Life Sciences
Earth’s biodiversity is undergoing substantial changes in response to anthropogenic stressors, creating a need for efficient methods of assessing biodiversity, particularly in soil ecosystems where sampling remains challenging. Soil vibroacoustics - the use of substrate-borne vibrations or sounds to quantify or detect below-ground biological activity – offers a potentially rapid and scalable [...]
Hive product metagenomics: a promising window into honey bee genomics and bee-associated ecosystems
Published: 2026-09-06
Subjects: Apiculture, Entomology, Genomics, Microbiology, Other Genetics and Genomics
Through their pollination services, bees are essential components of ecosystem functioning, supporting biodiversity and contributing to global food production. Among them, honey bees are particularly important, both for their ecological role and their widespread contribution to many agricultural systems and beekeeping. Honey, beeswax and propolis, among other beehive products, have played a [...]
A harmonised dataset of adult Culex pipiens mosquitoes from 17 administrative Italian regions, 2008–2022
Published: 2026-09-06
Subjects: Biology, Entomology
Culex pipiens s.l. is the primary vector of West Nile Virus in Italy. Despite the availability of abundant long-term surveillance data on adult Cx. pipiens observations in Italy, historical records are often fragmented across regional repositories with heterogeneous formats and metadata. This work introduces a comprehensive, harmonised dataset of adult Cx. pipiens observations collected across 17 [...]
TRIPLE-IJ and SHIFT as a coupled framework for characterising entomopathogenic nematode performance under abiotic stress
Published: 2026-08-26
Subjects: Agriculture, Entomology, Other Ecology and Evolutionary Biology, Research Methods in Life Sciences
Entomopathogenic nematodes (EPNs) of the families Steinernematidae and Heterorhabditidae are widely recognised as promising biological control agents, yet the translation of laboratory-demonstrated virulence into consistent field-scale efficacy remains an unresolved challenge. A central reason for this gap is the fragmented way EPN performance is conventionally characterised: infectivity and [...]
Increase, decrease, or both: A systematic review of mosquito population response to floods
Published: 2026-08-21
Subjects: Ecology and Evolutionary Biology, Entomology, Life Sciences, Parasitic Diseases, Terrestrial and Aquatic Ecology, Virus Diseases
Floods are increasing in frequency and severity under the ongoing climate change and are widely reported to alter mosquito population dynamics. However, the direction, magnitude, and consistency of this effect across mosquito species and flood contexts remain poorly studied. This systematic review synthesizes empirical evidence on mosquito species population responses to disastrous flood events [...]
Reproduction is more sensitive to warming than adult lifespan across invertebrates
Published: 2026-08-11
Subjects: Animal Sciences, Biodiversity, Entomology, Life Sciences, Population Biology, Terrestrial and Aquatic Ecology
Studies estimating the effect of temperature on wild animal populations typically focus on survival as a proxy for population health. However, high and low temperatures can also negatively influence animal reproduction, and recent work suggests that the thermal sensitivity of reproduction is more important for population persistence that the thermal sensitivity of survival in some species groups. [...]
How (not) to study insect microbiota in the age of high-throughput sequencing
Published: 2026-08-06
Subjects: Biodiversity, Ecology and Evolutionary Biology, Entomology, Environmental Microbiology and Microbial Ecology Life Sciences
The recognition of microbiota as a critical component of insect biology and evolution has driven a rapid expansion of insect-microbe interaction research. Yet while high-throughput sequencing has democratized access to microbial community profiling, it has also normalized low-resolution, artifact-prone approaches that often yield limited biological insights. Insects pose distinct challenges for [...]
Life-history traits do not always evolve in response to changing thermal environments
Published: 2026-08-04
Subjects: Biodiversity, Biology, Comparative and Evolutionary Physiology, Ecology and Evolutionary Biology, Entomology, Integrative Biology, Zoology
Quantifying animals' capacity to evolve in response to changing environments is central to predicting ecological responses to climate change. However, empirical evidence for evolutionary responses to changing temperatures remains mixed, and we know little about how selection alters trait variance or generates trade-offs among traits. Here, we conducted a systematic review and meta-analysis of 44 [...]
Continental breeding hotspots of migratory monarch butterflies revealed by coordinated community science partnership
Published: 2026-07-18
Subjects: Biodiversity, Ecology and Evolutionary Biology, Entomology
The eastern migratory monarch butterfly (Danaus plexippus plexippus) has declined by more than 80% over the past two decades, highlighting the need for improved understanding of its breeding ecology. To address longstanding gaps in continental-scale data on monarch reproduction, the International Monarch Monitoring Blitz has mobilized community scientists across North America to monitor monarch [...]
Insects as agents of national security: entomological biodiversity loss and ecosystem collapse in agriculture and forestry threaten geopolitical stability
Published: 2026-07-02
Subjects: Agriculture, Biodiversity, Biosecurity, Entomology, Forest Management, Forest Sciences, Life Sciences, Policy Design, Analysis, and Evaluation, Public Affairs, Public Policy and Public Administration
1. In early 2026, the UK Government published a report assessing how global biodiversity loss and ecosystem collapse represent systematic threats to UK national security through cascading impacts to food security, land use and climate-related feedbacks. 2. Recontextualising biodiversity and ecosystem health as determinants of national security offers a novel perspective on long observed [...]