Preprints
Filtering by Subject: Entomology
How (not) to study insect microbiota in the age of high-throughput sequencing
Published: 2026-08-06
Subjects: Biodiversity, Ecology and Evolutionary Biology, Entomology, Environmental Microbiology and Microbial Ecology Life Sciences
The recognition of microbiota as a critical component of insect biology and evolution has driven a rapid expansion of insect-microbe interaction research. Yet while high-throughput sequencing has democratized access to microbial community profiling, it has also normalized low-resolution, artifact-prone approaches that often yield limited biological insights. Insects pose distinct challenges for [...]
Life-history traits do not always evolve in response to changing thermal environments
Published: 2026-08-04
Subjects: Biodiversity, Biology, Comparative and Evolutionary Physiology, Ecology and Evolutionary Biology, Entomology, Integrative Biology, Zoology
Quantifying animals' capacity to evolve in response to changing environments is central to predicting ecological responses to climate change. However, empirical evidence for evolutionary responses to changing temperatures remains mixed, and we know little about how selection alters trait variance or generates trade-offs among traits. Here, we conducted a systematic review and meta-analysis of 44 [...]
Continental breeding hotspots of migratory monarch butterflies revealed by coordinated community science partnership
Published: 2026-07-18
Subjects: Biodiversity, Ecology and Evolutionary Biology, Entomology
The eastern migratory monarch butterfly (Danaus plexippus plexippus) has declined by more than 80% over the past two decades, highlighting the need for improved understanding of its breeding ecology. To address longstanding gaps in continental-scale data on monarch reproduction, the International Monarch Monitoring Blitz has mobilized community scientists across North America to monitor monarch [...]
Insects as agents of national security: entomological biodiversity loss and ecosystem collapse in agriculture and forestry threaten geopolitical stability
Published: 2026-07-02
Subjects: Agriculture, Biodiversity, Biosecurity, Entomology, Forest Management, Forest Sciences, Life Sciences, Policy Design, Analysis, and Evaluation, Public Affairs, Public Policy and Public Administration
1. In early 2026, the UK Government published a report assessing how global biodiversity loss and ecosystem collapse represent systematic threats to UK national security through cascading impacts to food security, land use and climate-related feedbacks. 2. Recontextualising biodiversity and ecosystem health as determinants of national security offers a novel perspective on long observed [...]
Joint species distributions reveal crop type and field-specific assembly rules and idiosyncrasies in carabid beetle occurrences and abundances
Published: 2026-06-25
Subjects: Entomology, Life Sciences, Other Ecology and Evolutionary Biology
1. Designing effective biodiversity conservation and pest regulation strategies in agroecosystems requires understanding how environmental gradients and assembly rules jointly structure ecological communities. 2. We fitted a hurdle joint species distribution model (jSDM) to presence–absence and conditional abundance data of 20 carabid species sampled across 57 arable fields in three French [...]
Beyond mistakes: same-sex partner acceptance and broad mating filters coexist in termite pairing
Published: 2026-06-24
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Entomology, Evolution
Same-sex sexual behavior is often interpreted either as a mistake arising from indiscriminate attempts or as an adaptive behavior directed towards same-sex partners. These explanations are typically considered mutually exclusive. Here we challenge this assumption using an adaptive same-sex pairing system in Reticulitermes termites. Long-term male-male pairings originate from tandem running, in [...]
EarthChirp: a global reference library for insect acoustic recognition and discovery across the audible and ultrasonic spectrum
Published: 2026-06-18
Subjects: Computational Biology, Ecology and Evolutionary Biology, Entomology, Genetics and Genomics
1. Passive acoustic monitoring (PAM) is scaling rapidly, but automated recognition for insects lags far behind birds. The dominant recogniser (BirdNET) classifies only ~35 insect species and building bespoke insect classifiers requires labelled training data that does not exist for most taxa. 2. We present EarthChirp, a training-free recogniser for singing insects (Orthoptera and Cicadidae) [...]
History, challenges, and opportunities in the study of entomopathogenic fungi in tropical regions: Borneo as a model ecosystem
Published: 2026-06-17
Subjects: Biodiversity, Entomology, Life Sciences, Other Ecology and Evolutionary Biology
Fungal pathogens tend to have a poor reputation as a disease among the general public and policy-makers. However, entomopathogenic fungi, adapted to infect and kill arthropod hosts, play a wide range of roles in ecosystems, provide key ecosystem services, and offer interesting models to understand pathogen interaction networks. Tropical regions provide especially favorable conditions for [...]
Four decades of inland invasion by Formosan subterranean termite in Alabama: expansion associated with transportation infrastructure
Published: 2026-06-17
Subjects: Entomology
Invasive termites pose ecological and economic concerns as both cellulose-destroying pests and ecosystem engineers. The Formosan subterranean termite, Coptotermes formosanus Shiraki, is listed among the world's worst invasive species and has established invasive populations across continents. Most studies have emphasized their transoceanic international dispersal and establishment in the coastal [...]
EntoScan and BEEomass: a standardized imaging system and a physically motivated model for high-throughput dry biomass estimation of arthropods
Published: 2026-06-12
Subjects: Biodiversity, Computational Biology, Computational Engineering, Ecology and Evolutionary Biology, Engineering, Entomology
Computer vision and AI are now widely used for automated insect classification, but their potential for estimating other traits, such as biomass, is not yet fully explored. Insect biomass is a key measure of ecosystem function, informing ecosystem services, food webs, and environmental change. It is also used to track population trends and estimate the contribution of insects to ecosystem carbon. [...]
Predator Experience Shapes Behaviour: Comparing Stone Wētā (Hemideina maori) Populations With and Without Weka (Gallirallus australis hectori)
Published: 2026-06-10
Subjects: Biology, Ecology and Evolutionary Biology, Entomology, Life Sciences
Antipredator behaviour reflects both evolutionary history and individual experience, yet how populations respond to changes in predator exposure remains poorly understood, particularly for large invertebrates. We examined antipredator behaviour in two populations of stone wētā (Hemideina maori Pictet & Saussure, 1891) inhabiting weka-free Mou Tapu and nearby Mou Waho, where weka (Gallirallus [...]
Symbiont interactions bias measures of arthropod biodiversity
Published: 2026-06-06
Subjects: Biodiversity, Entomology
Although arthropods comprise the majority of all named species, their biodiversity is relatively understudied. Here, we highlight how interactions between arthropods and their bacterial symbionts can pose challenges for biodiversity research, especially for estimating taxonomic, functional, and genetic diversity. We also argue that recent technological developments and biomonitoring schemes [...]
Trophic interactions of ants are robust to tree species loss
Published: 2026-06-05
Subjects: Entomology, Life Sciences, Terrestrial and Aquatic Ecology
1. How changes in habitat conditions influence insect diversity has been intensively studied. However, whether trophic interactions of insects are also influenced by such changes is largely unknown. Higher habitat heterogeneity is often hypothesized to promote niche partitioning and complementarity in resource use among interacting species, yet evidence from animal interaction networks is sparse. [...]
Evaluating the potential of molecular dietary analysis of predators for the detection of emerging plant pests
Published: 2026-06-01
Subjects: Agriculture, Behavior and Ethology, Biodiversity, Biosecurity, Ecology and Evolutionary Biology, Entomology, Forest Biology, Forest Management, Forest Sciences, Genetics, Laboratory and Basic Science Research Life Sciences, Life Sciences, Research Methods in Life Sciences
Monitoring plant pests is crucial for maximising yields across agricultural and forest production systems, but also for the mitigation of invasive species spread. Traditional monitoring methods, such as mass trapping and direct observation, scale poorly and introduce latency between collection, detection and response. Since many plant pests are frequently consumed by predators, molecular dietary [...]
Canopy closure re-establishes ants in young tree plantations, while low soil pH limits ant diversity
Published: 2026-05-28
Subjects: Entomology, Life Sciences, Terrestrial and Aquatic Ecology
1. Tree species richness is known to enhance biodiversity and ecosystem functioning, but its effects across trophic levels during forest restoration remain insufficiently understood. In reforestation on complex terrain, habitat complexity may moderate the effect of canopy closure on animal community reassembly, a relationship further shaped by the abiotic environment. 2. Ants, as key functional [...]