Preprints
Filtering by Subject: Entomology
DrawerDissect: Whole-drawer insect imaging, segmentation, and transcription using AI
Published: 2025-07-15
Subjects: Artificial Intelligence and Robotics, Biology, Ecology and Evolutionary Biology, Entomology, Life Sciences
1. Natural history museums often curate large collections of pinned insects. These collections represent invaluable records of biodiversity information, ecological patterns and phenotypic variation. A common goal of museums is to create digital versions of these records for curation and research purposes. However, traditional methods of specimen imaging and metadata transcription are [...]
Emerging tools to advance neuroethology in butterflies and moths
Published: 2025-06-30
Subjects: Behavior and Ethology, Entomology, Other Ecology and Evolutionary Biology
Butterflies and moths have played historically important roles in developing our understanding of both ecology and evolutionary biology, and neuroethology. In both contexts, the diversity of behavioral strategies and specializations displayed by different Lepidoptera make them informative case studies. However, as in neuroscience more broadly, lepidopteran neuroethology has tended to focus on [...]
Two Metschnikowia nectar yeast species have similar volatile profiles, but elicit differential foraging in bee pollinators
Published: 2025-06-19
Subjects: Ecology and Evolutionary Biology, Entomology, Life Sciences, Microbiology, Organismal Biological Physiology, Other Microbiology
Nectar yeasts are a highly specialized group of fungi that may play key roles in pollination ecology. Nectar yeasts lack an independent dispersal mechanism to access new habitats with fresh resources. Yeasts, bumble bee pollinators, and flowering plants likely take part in a series of diffuse mutualisms, wherein yeast attract bees that provide phoretic travel between flowers. This interaction [...]
Elevating the importance of Risk of Bias assessment for ecology and evolution
Published: 2025-06-17
Subjects: Agriculture, Animal Sciences, Biodiversity, Biology, Ecology and Evolutionary Biology, Entomology, Life Sciences, Marine Biology, Microbiology, Plant Sciences, Research Methods in Life Sciences
Systematic reviews and meta-analyses are key evidence synthesis methods informing research and policy. An assessment of the Risk of Bias (RoB) in included studies is normally considered an essential component of these. However, RoB assessment is rare in ecology and evolutionary biology (EEB), and tools from other fields are seldom adopted. To identify reasons for this limited uptake, we surveyed [...]
Cellular Innovations and Diversity in the Lepidopteran Compound Eye
Published: 2025-05-28
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Entomology, Evolution, Life Sciences, Molecular Genetics
Lepidoptera, having co-diversified with flowering plants and adapted to various diel niches, present a remarkable system for studying compound eye cell type diversity. Here we synthesize the latest research regarding lepidopteran eye evolution across different timescales, from species-level variation to family-level changes, and mechanistic levels, from broad anatomical variation to molecular [...]
Filling Monitoring Gaps for Data-deficient Species Using Annual Occupancy Predictions from Co-occurrence Models
Published: 2025-05-21
Subjects: Biodiversity, Ecology and Evolutionary Biology, Entomology, Life Sciences
Fragmented surveys and limited monitoring have excluded most invertebrates from conservation policy. We present a fill-in framework that uses species distribution models (SDMs) to reconstruct missing annual trends—not to extrapolate trends, but to fill them in. Instead of filtering data-sparse regions or years or relying on static environmental variables, we used co-occurrence patterns (COP) as [...]
Proximity to natural habitat is not consistently associated with pollination services in tropical smallholder farms: A systematic review and meta-analysis
Published: 2025-05-13
Subjects: Agriculture, Biodiversity, Ecology and Evolutionary Biology, Entomology
Proximity to natural habitat is known to enhance pollination services in large-scale agriculture, but it remains unclear whether this holds in tropical smallholder farms. These systems are embedded in ecologically complex landscapes, central to global food security, and depend heavily on biodiversity-derived ecosystem services. We conducted a systematic review and meta-analysis of 35 studies [...]
Facing the heat: behavioral and molecular underpinnings of heat stress in bumblebees
Published: 2025-04-25
Subjects: Animal Studies, Behavioral Neurobiology, Behavior and Ethology, Ecology and Evolutionary Biology, Entomology, Environmental Studies, Life Sciences, Neuroscience and Neurobiology, Social and Behavioral Sciences
Climate change heralds an era of increased heat waves. Insects, due to their short generation times and their sensitive ecological requirements, offer a powerful model for studying rapid physiological and behavioral responses to high temperatures. Solitary insects primarily respond to temperature extremes by moving in space or time to remain in a constant environment, or by exploiting phenotypic [...]
Speciation in ants: Unlocking ant diversity to study speciation (Hymenoptera: Formicidae)
Published: 2025-04-09
Subjects: Ecology and Evolutionary Biology, Entomology, Life Sciences, Other Ecology and Evolutionary Biology
Ants are among the most abundant terrestrial animals and play key roles in ecosystems across the globe. Their taxonomic and ecological diversity makes them a prime taxon for contributing to our understanding of the patterns and processes of speciation. However, studies on ants, such as those on ant diversification or taxonomy, often do not explicitly consider how their results inform or update [...]
AedesTraits: A global database of temperature–dependent trait responses in Aedes mosquitoes
Published: 2025-04-08
Subjects: Biology, Entomology, Life Sciences
Invasive Aedes mosquitoes are major vectors of arboviral diseases such as dengue, Zika, and chikungunya, posing an increasing threat to global public health. Their recent geographic expansion calls for predictive models to simulate population dynamics and transmission risk. Temperature is a key driver in these models, influencing traits that affect vector competence. While data on [...]
Pharmacophagy in Insects: Ecological and Evolutionary Perspectives on the Non-Nutritional Use of Plant Specialized Metabolites
Published: 2025-04-02
Subjects: Ecology and Evolutionary Biology, Entomology, Life Sciences
Herbivorous insects can interact with plants in ways that go beyond nutrition, with plant specialized (secondary) metabolites (PSMs) mediating complex non-nutritional relationships. While PSMs often function as anti-herbivore defenses, many insects have evolved strategies to counteract and even exploit these compounds, using them for purposes such as their own defense against antagonists, [...]
Habitat filtering, not dispersal limitation, drives ant and termite community assembly along a tropical forest regeneration gradient
Published: 2025-03-27
Subjects: Biodiversity, Ecology and Evolutionary Biology, Entomology, Life Sciences
Regenerating forests comprise a significant proportion of forest ecosystems in the tropics. However, species assembly mechanisms after anthropogenic disturbances are still poorly understood. It has been shown that locally established ant communities clearly assemble along gradients of forest regeneration. However, it is unclear if this is determined by dispersal limitation or habitat filtering [...]
Modelling the distribution of the tick Ixodes ricinus in England and Wales using passive surveillance data from citizen science reports
Published: 2025-03-07
Subjects: Ecology and Evolutionary Biology, Entomology, Environmental Public Health, Public Health
Background: The tick Ixodes ricinus is the most common tick species in the UK and a significant vector of Borrelia burgdorferi s.l. (causative agent of Lyme borreliosis) and Tick-Borne Encephalitis virus (TBEv) to humans and Anaplasma phagocutphilum, Babesia divergens and louping ill virus to animals.Methods: The Tick Surveillance Scheme (TSS) administered by the UK Health Security Agency (UKHSA) [...]
Agroecological farming promotes yield and biodiversity but may require subsidy to be profitable
Published: 2025-03-04
Subjects: Agriculture, Biodiversity, Ecology and Evolutionary Biology, Entomology, Life Sciences, Other Ecology and Evolutionary Biology
1. Intensive arable agriculture uses agrochemicals to replace ecosystem services (e.g. pest control and soil health) while simultaneously degrading others (e.g. pollination). Agroecological farming aims to reduce this reliance. Whether these practices maintain yields at a scale relevant to farm business viability is unclear. 2. In a 4-year replicated study across 17 English farms we assessed [...]
Transferable approaches to CRISPR-Cas9 induced genome editing in non-model insects: a brief guide
Published: 2025-02-27
Subjects: Animal Experimentation and Research, Animal Sciences, Entomology, Genetics, Life Sciences, Molecular Genetics, Other Genetics and Genomics, Zoology
Despite the large variety of insect species with divergent morphological, developmental and physiological features questions on gene function could for a long time only be addressed in few model species. The adoption of the bacterial CRISPR-Cas system for genome editing in eukaryotic cells widened the scope of the field of functional genetics: for the first time the creation of heritable genetic [...]