Preprints
Filtering by Subject: Genomics
Alternative Promoters Expand Transcriptional Complexity of Temperature Stress Responses in Cassava
Published: 2025-09-29
Subjects: Agriculture, Bioinformatics, Genetics and Genomics, Genomics, Plant Biology, Plant Sciences
Plant abiotic stress responses involve two major gene expression regulatory mechanisms: alternative promoter usage and differential expression. Although differentially expressed genes (DEGs) have been extensively studied, alternative promoter genes (APGs) remain poorly characterized despite their potential importance. We systematically compared APGs and DEGs in cassava (Manihot esculenta), a [...]
Project Psyche: Generating and utilising reference genomes for all Lepidoptera in Europe
Published: 2025-09-10
Subjects: Biodiversity, Genetics and Genomics, Genomics, Life Sciences
Project Psyche is a trans-national initiative to generate and study chromosome-level reference genomes of all ca. 11,000 described species of Lepidoptera (butterflies and moths) found in Europe. The Project Psyche community encompasses diverse researchers, amateur lepidopterists, practitioners, and industry experts united by a common vision of the importance of genomics for Lepidoptera. [...]
Predictive Evolutionary Genomics: Principles, Validation, and Practice
Published: 2025-08-10
Subjects: Agriculture, Biodiversity, Ecology and Evolutionary Biology, Evolution, Genomics, Life Sciences, Plant Sciences, Population Biology
Contemporary evolution occurs on observable timescales, enabling prospective evolutionary forecasting with quantitative frameworks rather than only retrospective inference. We propose a unified probabilistic framework that integrates three approaches linked to detectability windows through time. Trait-based models use multivariate quantitative-genetic equations to project correlated phenotypic [...]
Toxin resistance mechanisms span biological scales in the Royal Ground Snake (Colubridae: Erythrolamprus reginae)
Published: 2025-06-13
Subjects: Animal Sciences, Behavior and Ethology, Biology, Cellular and Molecular Physiology, Comparative and Evolutionary Physiology, Ecology and Evolutionary Biology, Evolution, Genomics, Integrative Biology, Life Sciences, Molecular Biology, Neuroscience and Neurobiology, Physiology, Systems and Integrative Physiology Life Sciences, Systems Biology, Zoology
All predators of poisonous prey deal with toxins, yet we often lack an understanding of how the predators integrate various physiological adaptations to toxins. Here we investigate multiple complementary toxin resistance mechanisms in the pan-Amazonian snake Erythrolamprus reginae (Squamata: Colubridae), which consumes multiple species of poisonous frogs. We find that the toxin resistance [...]
Inescapable bottlenecks: Demographic recovery cannot replenish evolutionary potential over conservation timeframes
Published: 2025-06-13
Subjects: Ecology and Evolutionary Biology, Genomics, Life Sciences, Molecular Genetics, Population Biology
Maintaining within-species genetic diversity is a central goal of biodiversity conservation, because it determines a species’ capacity to adapt to environmental change. Species that have experienced population bottlenecks are expected to suffer from an increased risk of extinction unless their genetic diversity is able to recover. A recent study proposed that rapid demographic expansion in a [...]
A draft genome assembly for the dart-poison frog Phyllobates terribilis
Published: 2025-06-10
Subjects: Biology, Genomics
Dendrobatid poison frogs have become well established as model systems in several fields of biology. Nevertheless, the development of molecular and genetic resources for these frogs has been hindered by their large, highly repetitive genomes, which have proven difficult to assemble. Here we present a draft assembly for Phyllobates terribilis (12.6Gb), generated using a combination of sequencing [...]
IQ2MC: A New Framework to Infer Phylogenetic Time Trees Using IQ-TREE 3 and MCMCTree with Mixture Models
Published: 2025-05-05
Subjects: Bioinformatics, Computational Biology, Ecology and Evolutionary Biology, Evolution, Genetics, Genetics and Genomics, Genomics, Life Sciences
IQ-TREE and MCMCtree are two widely used tools for inferring phylogenetic trees and estimating divergence times, respectively. As MCMCtree performs fast approximate Markov Chain Monte Carlo sampling to obtain the times along a fixed tree topology, it would be natural to use IQ-TREE to obtain the tree. However, integrating these tools seamlessly was not previously possible, as MCMCtree requires [...]
Plant genomic variation and its implications for proposed EU NGT legislation
Published: 2025-04-09
Subjects: Biotechnology, Ecology and Evolutionary Biology, Evolution, Genetics, Genetics and Genomics, Genomics, Life Sciences, Molecular Genetics, Plant Breeding and Genetics Life Sciences, Plant Sciences
The European Commission proposal for New Genomic Techniques (NGTs) of July 2023 specifies that NGT1 plants, which are considered equivalent to conventional plants, may differ from the recipient or parental plant by no more than 20 insertions, which cannot be longer than 20 bp; deletions can be of any size and number. Here, we examine the proposed 20/20 NGT1 limit against the background of the [...]
De Novo Gene Emergence: Summary, Classification, and Challenges of Current Methods
Published: 2025-04-07
Subjects: Bioinformatics, Genomics
A novel mechanism of de novo gene origination from non-genic sequences was first proposed in the early 2000s. Subsequent studies have since provided evidence of de novo gene emergence across all domains of life, revealing its occurrence to be more frequent than initially anticipated. While studies mainly agree on the general concept of de novo emergence from non-genic DNA, the exact methods and [...]
A Novel Landscape Macrogenetics Approach Reveals Conservation Implications of Australia's 2019–2020 Black Summer Wildfires
Published: 2025-03-17
Subjects: Biodiversity, Genetics, Genomics, Life Sciences, Molecular Genetics, Other Ecology and Evolutionary Biology, Population Biology
AimThe use of genetic analyses has become ubiquitous in conservation planning and management. Typically, such analyses are employed at the species-level, though as genetic data accrue, it is now possible to consider the genetic composition of multiple species across landscapes. Such macrogenetic perspectives can reveal the potential genetic ramifications of extreme disturbance events, such as the [...]
Environmental RNA mitigates fish ghosts related to fish feeds for aquaculture in molecular ecological survey in a bay.
Published: 2024-12-19
Subjects: Aquaculture and Fisheries Life Sciences, Biodiversity, Genomics, Terrestrial and Aquatic Ecology
The use of environmental DNA for molecular ecological surveys has become widely utilized in ecological assessments of various water environments, from rivers to coastal and marine areas. However, it is important to consider the scenarios of potential contamination of environmental nucleic acids in interpreting the results. In this study, we analyzed the fish species present in the feed that may [...]
Unlocking the hidden dimensions of genomic diversity within species
Published: 2024-12-16
Subjects: Evolution, Genomics, Molecular Biology
The missing heritability problem, defined as the failure of genetic variants to explain variance in phenotypes, has been an unsolved issue in genetics for the past two decades. A potential solution to this problem stems from the idea that single nucleotide polymorphisms and copy-number variants, the most commonly studied forms of genomic diversity, do not represent the totality of the information [...]
Reassessing a Holocene extinction: multiple lines of evidence do not support the historical presence and recent extirpation of a protected anole on the island of Anguilla
Published: 2024-09-20
Subjects: Biodiversity, Genomics, Integrative Biology, Other Ecology and Evolutionary Biology, Zoology
Accurate assessment of historical species ranges is important for conservation science and management. Inaccurate historical species ranges can lead to incorrect assumptions about local extinctions, population trends, and potential sites for reintroductions. Yet, historical knowledge is often lacking for many species. Here, we examine the case of the bearded anole, Anolis pogus, which is long [...]
Demographic expansion and panmixia in a St. Martin endemic, Anolis pogus, coincides with the decline of a competitor
Published: 2024-09-19
Subjects: Biodiversity, Ecology and Evolutionary Biology, Evolution, Genomics, Population Biology, Zoology
Understanding patterns of differentiation at microgeographic scales can enhance our understanding of evolutionary dynamics and lead to the development of effective conservation strategies. In particular, high levels of landscape heterogeneity can strongly influence species abundances, genetic structure, and demographic trends. The bearded anole, Anolis pogus, is endemic to the topographically [...]
Not by selection alone: expanding the scope of gene-culture coevolution
Published: 2024-09-05
Subjects: Anthropology, Biological and Physical Anthropology, Ecology and Evolutionary Biology, Evolution, Genetics and Genomics, Genomics, Social and Behavioral Sciences
Gene-culture coevolution (GCC) - an ambitious synthesis of biological and social sciences - is often used to explain the evolution of key human traits. Despite the framework’s broad conceptual appeal however, empirical evidence is often perceived as limited to a few key examples like lactase persistence. We argue this apparent gap between theoretical appeal and empirical evidence stems [...]