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Preprints

Filtering by Subject: Biology

varKodes extend DNA barcoding to ultrafine-grained identity, place, and state

Peter J Flynn, Jackson Kehoe, T. Jonathan Davies, et al.

Published: 2026-07-23
Subjects: Biodiversity, Bioinformatics, Biology, Ecology and Evolutionary Biology, Genetics and Genomics, Life Sciences

DNA barcoding has transformed biodiversity science, but locus-based markers often fail across taxa, at shallow phylogenetic depths, and in mixed or spatially structured samples. Here, we test whether varKoder, an alignment-free framework that converts low-coverage genomic reads into image-based genomic signatures (varKodes), can extend barcoding from taxonomic identification to broader inference [...]

Species Traits Predict Local Population Change Above and Beyond Regional Trend

Ivara Goulden, Casey Youngflesh, Allen H Hurlbert

Published: 2026-07-23
Subjects: Biology, Ecology and Evolutionary Biology, Population Biology

Why do some populations persist in the face of environmental change while others decline? We tested the extent to which variation in local population change across species is attributable to three species traits: (i) habitat selectivity, (ii) temperature niche position, and (iii) degree of insectivory, with the null hypothesis that local trends are simply a reflection of broader regional trends. [...]

Swept ashore: Transoceanic dispersals and the biogeographic origins of Australian gekkonid lizards

Wesley Read, Ian Brennan, Sarin Tiatragul, et al.

Published: 2026-07-20
Subjects: Biology, Life Sciences

Oceanic dispersal is a rare mode of long-distance dispersal where taxa colonize disconnected islands or continents, sometimes resulting in new radiations. Australia has been isolated from other continental landmasses during the last 45 million years, and overwater colonisation has played a critical role in shaping its contemporary biota. Here, we investigate the biogeographic origins of [...]

Is Biology Necessary to Advance Biology?

John T Van Stan, II, Theodore Bach, Olivier Dangles, et al.

Published: 2026-07-14
Subjects: Biochemistry, Biophysics, and Structural Biology, Biodiversity, Bioinformatics, Biology, Cell and Developmental Biology, Computational Biology, Philosophy, Scholarship of Teaching and Learning, Systems Biology

AI is transforming the workflow of biological science. But what role remains for human cognition when the priority is deep theory? We propose a division of labor based on a “coverage asymmetry” between artificial and biological agents. AI excels at extending the encounterable: scaling known mechanisms and exploring complex parameter regimes. However, AI cannot (yet) perform kind formation – the [...]

Sniffing for fungi: Use of a conservation dog uncovers high regional truffle diversity

Heather A Dawson, Jonathan L. Frank, Carolyn Delevich, et al.

Published: 2026-07-09
Subjects: Biodiversity, Biology, Botany, Ecology and Evolutionary Biology, Environmental Microbiology and Microbial Ecology Life Sciences, Forest Biology, Forest Sciences, Plant Sciences, Research Methods in Life Sciences, Terrestrial and Aquatic Ecology

Hypogeous aromatic fungi (‘truffles’) contribute significantly to overall fungal diversity but are difficult to find using traditional survey methods because they fruit underground, leading to under-documentation and a lack of understanding of truffle ecology. Truffles evolved to emit strong aromatic compounds to attract mycophagists for spore dispersal, a trait that culinary truffle harvesters [...]

Avian Population Genomics: Latest Findings and Future Prospects

Georg Langebrake, Kira Delmore, Miriam Liedvogel

Published: 2026-06-25
Subjects: Biology, Ecology and Evolutionary Biology, Genetics and Genomics, Life Sciences

Birds are one of the most recognizable and diverse groups of organisms on Earth. This group has played an important role in many fields, including the development of methods in behavioral ecology and evolutionary theory. The use of population genomics took off following the increased accessibility of high-throughput sequencing across taxa. Several features of bird genomes make them particularly [...]

Parasitic zoosporic eufungi: taxonomic and phylogenetic diversity, ecology, and impacts

Kensuke Seto, Kathryn T. Picard, Gustavo H. Jerônimo, et al.

Published: 2026-06-17
Subjects: Biodiversity, Biology, Parasitology, Plant Pathology

Zoosporic eufungi (i.e., chytrids, sensu lato) comprise a phylogenetically and ecologically diverse guild of early diverging fungal phyla (Chytridiomycota, Monoblepharomycota, Neocallimastigomycota, Blastocladiomycota, Sanchytriomycota, Aphelidiomycota, Cryptomycota/Rozellomycota, and Olpidiomycota). While most circumscribed zoosporic eufungi function as decomposers of recalcitrant materials, [...]

An integrated framework for unifying our understanding of nonconsumptive predation risk effects

Andrew Thomas Davidson, Tal Avgar, Daniel MacNulty, et al.

Published: 2026-06-12
Subjects: Behavior and Ethology, Biology, Ecology and Evolutionary Biology, Evolution, Integrative Biology, Life Sciences, Other Ecology and Evolutionary Biology, Population Biology, Terrestrial and Aquatic Ecology

Predation risk can induce risk-induced trait responses (RITRs) – changes in prey defensive traits including behavior, morphology, life history, and physiology – thought to have profound effects on prey fitness and population dynamics (termed ‘nonconsumptive effects’). Yet, predicting the magnitude of RITRs and their fitness consequences remains difficult because outcomes depend heavily on [...]

Nest architecture as overlooked material culture: the case for systematic study of construction behaviour across nest-building primates

Andrea L. Permana

Published: 2026-06-10
Subjects: Animal Studies, Anthropology, Biological Psychology, Biology, Comparative Psychology, Ecology and Evolutionary Biology

Frans de Waal's work highlighted an uncomfortable question: not whether animals have complex cognitive lives, but why we are so reluctant to recognise them. Nest building in great apes is perhaps the most striking example of this problem. Every great ape builds a nest, every day, for the entirety of its adult life. The behaviour has been documented ecologically for decades. Yet the internal [...]

Predator Experience Shapes Behaviour: Comparing Stone Wētā (Hemideina maori) Populations With and Without Weka (Gallirallus australis hectori)

Sheri Johnson, Luke Thompson, Hamish Doogan, et al.

Published: 2026-06-10
Subjects: Biology, Ecology and Evolutionary Biology, Entomology, Life Sciences

Antipredator behaviour reflects both evolutionary history and individual experience, yet how populations respond to changes in predator exposure remains poorly understood, particularly for large invertebrates. We examined antipredator behaviour in two populations of stone wētā (Hemideina maori Pictet & Saussure, 1891) inhabiting weka-free Mou Tapu and nearby Mou Waho, where weka (Gallirallus [...]

Predicting substrate size at a watershed scale to inform conservation planning for a declining salmonid species

Kyleisha J. Foote, Shawn J. Leroux, Ava J. Hart, et al.

Published: 2026-06-03
Subjects: Biology, Earth Sciences, Ecology and Evolutionary Biology, Environmental Sciences, Hydrology, Life Sciences, Natural Resources and Conservation, Physical Sciences and Mathematics

Good quality spawning habitat is critical for fish embryo development, survival, and overall population productivity. Appropriate riverbed substrate size is particularly important for riverine-spawning salmonids but the availability of suitable substrate may vary across a watershed. Predicting substrate size at watershed extents may therefore be critical to inform management and conservation of [...]

From social experience to social behaviour: hormonal and behavioural phenotypes during adolescence in male guinea pigs

Melanie Gleske, S. Helene Richter, Sylvia Kaiser

Published: 2026-06-03
Subjects: Animal Sciences, Biology, Life Sciences, Social and Behavioral Sciences

Adolescence is the transition from juvenility to adulthood and is characterized by prominent endocrine, neural and behavioural alterations. Thus, adolescence represents a sensitive phase during which social experiences can shape endocrine and behavioural phenotypes. Although the influence of the social environment during adolescence has been widely investigated, most studies assessed such effects [...]

From Individuals to Networks: The Role of Variation in Plant-Pollinator Communities' Responses to Global Change

James DeWitt Crall, Marilia Gaiarsa

Published: 2026-05-28
Subjects: Biology, Ecology and Evolutionary Biology, Integrative Biology, Life Sciences

1. Plant–pollinator communities are critical for biodiversity, ecosystem function, and human well-being. Yet our ability to predict divergent species responses to environmental change, the risk of abrupt collapse, or the potential for recovery in plant-pollinator systems remains limited. 2. Here, we argue that individual variation within species may play a critical but underappreciated role in [...]

Inferring genomic landscapes with the integrative sequentially Markov coalescent (iSMC)

Gustavo Valadares Barroso, Julien Yann Dutheil

Published: 2026-05-28
Subjects: Bioinformatics, Biology, Computational Biology, Genetics and Genomics, Genomics, Life Sciences

The integrative Sequentially Markovian Coalescent (iSMC) is an extension of the sequentially Markovian Coalescent (SMC) model allowing for parameter heterogeneity along the genome, such as recombination and mutation rates. Heterogeneous parameters follow an autocorrelation process that modulates the genealogical process, extending the hidden state space and adding as few as two extra parameters [...]

A viral mimic increases body temperature but does not affect mass or inflammation in a wild frugivorous bat

Alexis Heckley, Valeriia Bohodist, Camilo Calderon, et al.

Published: 2026-05-28
Subjects: Biology, Life Sciences

The acute phase response is a component of innate immunity that helps fight infections. Understanding variation in this response is particularly critical in bats, which can be asymptomatic hosts of pathogens that cause disease in other animals. Although bats are most famously tolerant of viruses, research on the bat acute phase response has focused predominantly on bacterial antigens. To improve [...]

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