Preprints
Filtering by Subject: Biology
Beyond the Vertebrate Model: Insights from Insect Stress Responses
Published: 2026-10-15
Subjects: Animal Sciences, Animal Studies, Behavioral Neurobiology, Behavior and Ethology, Biology, Cell and Developmental Biology, Cellular and Molecular Physiology, Cognitive Neuroscience, Comparative and Evolutionary Physiology, Ecology and Evolutionary Biology, Entomology, Evolution, Genetics, Molecular Genetics, Systems Biology, Zoology
Stress responses are essential for survival, coordinating behavioural, physiological and molecular adjustments that enable organisms to cope with challenges. Most research has focused on vertebrates, leaving invertebrate stress physiology comparatively underexplored, particularly in insects. In this review, we compare insect and vertebrate stress responses to examine what insects reveal about the [...]
Frequent recovery of Sydowia polyspora from brown spot needle blight-associated loblolly pine (Pinus taeda) needles in Alabama
Published: 2026-10-13
Subjects: Biology, Genetics and Genomics, Plant Pathology
Sydowia polyspora is a widespread conifer-associated fungus that can function as an endophyte, saprophyte, or opportunistic pathogen, but its occurrence in southeastern pine systems remains poorly characterized. During surveys of symptomatic loblolly pine (Pinus taeda) in Washington and Cullman counties, Alabama, fungal isolates morphologically consistent with Sydowia spp. were recovered from [...]
Where are the hake going? How age, environmental signals, and shelf habitat shape Pacific Hake (Merluccius productus) summer distribution
Published: 2026-10-13
Subjects: Biology, Ecology and Evolutionary Biology, Marine Biology
Sustainably managing a multinational fishery on a transboundary, relatively long-lived and migratory fish stock is challenging, especially in a changing ocean environment. In a warming world, Pacific hake (Merluccius productus, Merluccidae), jointly managed by the U.S. and Canada, have been hypothesized to move north. However, in recent years the proportion of Pacific hake in the northern part of [...]
INSTAR: reporting items for invertebrate welfare in research
Published: 2026-09-24
Subjects: Animal Sciences, Biology, Ecology and Evolutionary Biology, Entomology, Life Sciences
Concern for the welfare of research animals has expanded rapidly across taxa, with increasing focus on invertebrates. Recent evidence for affective experience in cephalopod, decapod and insect taxa, alongside shifts in public attitudes and regulation, has raised the stakes of how invertebrates are used and reported, yet no reporting standard adequately addresses them. Here we present INSTAR [...]
As invasions slow down, biosecurity must move inward
Published: 2026-09-21
Subjects: Animal Studies, Biology, Ecology and Evolutionary Biology, Environmental Studies
Rates of non-native-species first records and establishment are slowing. This is encouraging, but it changes rather than resolves the biosecurity problem imposed by biological invasions. As external establishments decline while national non-native-species pools remain compositionally distinct, a growing share of remaining invasion opportunity should lie in redistribution among connected [...]
Multiple genetic routes to pelvic spine loss in brook stickleback
Published: 2026-09-10
Subjects: Biology, Ecology and Evolutionary Biology, Evolution, Life Sciences
Pelvic spine loss has evolved repeatedly in stickleback species and represents an intriguing opportunity to study genetically parallel adaptation. While previous work has identified key loci associated with this trait, the extent to which different populations rely on the same genetic mechanisms remains unclear. The genetic basis of pelvic spine loss in Culaea inconstans (Kirtland 1840) has only [...]
A harmonised dataset of adult Culex pipiens mosquitoes from 17 administrative Italian regions, 2008–2022
Published: 2026-09-07
Subjects: Biology, Entomology
Culex pipiens s.l. is the primary vector of West Nile Virus in Italy. Despite the availability of abundant long-term surveillance data on adult Cx. pipiens observations in Italy, historical records are often fragmented across regional repositories with heterogeneous formats and metadata. This work introduces a comprehensive, harmonised dataset of adult Cx. pipiens observations collected across 17 [...]
What goes up, must come down: air–soil microbiome connectivity and its implications for ecosystem functioning
Published: 2026-08-28
Subjects: Biodiversity, Biology, Ecology and Evolutionary Biology, Life Sciences, Microbiology
Soils harbor Earth's largest reservoir of microbial diversity, yet enormous numbers of microorganisms are continuously aerosolized, transported through the atmosphere, and redeposited across landscapes. Although soil and the atmosphere have largely been studied separately, here, we propose a conceptual framework that views soils and the atmosphere as a coupled microbiome linked by bidirectional [...]
Cell-autonomous immunity and the endosymbiotic theory: Septins at the origin?
Published: 2026-08-28
Subjects: Biology, Cell and Developmental Biology, Evolution, Life Sciences
The origin of complex eukaryotic cells remains a long-standing mystery. Endosymbiosis gave rise to mitochondria and chloroplasts, the bacteria-derived organelles that underpin eukaryotic productivity. Early eukaryotes had to establish these partnerships while avoiding pathogens, using limited ancestral tools. Septins are cytoskeletal GTP-binding proteins best known for roles in cytokinesis, yet [...]
Computational classification of SOD2 protein in coral and symbiotic algae from public genomic and transcriptomic data
Published: 2026-08-20
Subjects: Bioinformatics, Biology, Life Sciences, Marine Biology, Other Ecology and Evolutionary Biology
SOD2, or Manganese/Iron Superoxide Dismutase, plays a crucial role in maintaining cellular health across the tree of life. Existing literature is vague in identifying types of SOD2 proteins expressed in both corals and their symbiotic algae; most agree on the presence of a) mitochondrial targeting MnSOD in corals, and b) likely Mn dependent SOD2 in algae. This paper presents evidence for four [...]
Sleep elasticity: accessory, useful and vital components of a single state
Published: 2026-08-19
Subjects: Biology, Ecology and Evolutionary Biology, Evolution, Neuroscience and Neurobiology, Systems Biology
Sleep is universal, homeostatically defended and apparently costly, and for half a century this combination has been read as evidence of a vital, conserved function still awaiting identification. The search has not converged. Instead, it has accumulated a dozen candidate functions, each defensible but none commanding assent. I argue that the difficulty lies with the question rather than with the [...]
The knowledge landscape of jaguar science: rapid growth, thematic structure, and regional inequalities
Published: 2026-08-17
Subjects: Biodiversity, Biology, Ecology and Evolutionary Biology, Life Sciences
Understanding how scientific effort is distributed is essential for evaluating the evidence available to guide species conservation. We assessed the temporal, thematic, methodological, and spatial organization of jaguar (Panthera onca) research across the species’ range. Using 1,028 bibliographic records, we applied a metadata-based scope filter, defining a primary analytical corpus of 857 [...]
Life-history traits do not always evolve in response to changing thermal environments
Published: 2026-08-04
Subjects: Biodiversity, Biology, Comparative and Evolutionary Physiology, Ecology and Evolutionary Biology, Entomology, Integrative Biology, Zoology
Quantifying animals' capacity to evolve in response to changing environments is central to predicting ecological responses to climate change. However, empirical evidence for evolutionary responses to changing temperatures remains mixed, and we know little about how selection alters trait variance or generates trade-offs among traits. Here, we conducted a systematic review and meta-analysis of 44 [...]
varKodes extend DNA barcoding to ultrafine-grained identity, place, and state
Published: 2026-07-23
Subjects: Biodiversity, Bioinformatics, Biology, Ecology and Evolutionary Biology, Genetics and Genomics, Life Sciences
DNA barcoding has transformed biodiversity science, but locus-based markers often fail across taxa, at shallow phylogenetic depths, and in mixed or spatially structured samples. Here, we test whether varKoder, an alignment-free framework that converts low-coverage genomic reads into image-based genomic signatures (varKodes), can extend barcoding from taxonomic identification to broader inference [...]
Species Traits Predict Local Population Change Above and Beyond Regional Trend
Published: 2026-07-23
Subjects: Biology, Ecology and Evolutionary Biology, Population Biology
Why do some populations persist in the face of environmental change while others decline? We tested the extent to which variation in local population change across species is attributable to three species traits: (i) habitat selectivity, (ii) temperature niche position, and (iii) degree of insectivory, with the null hypothesis that local trends are simply a reflection of broader regional trends. [...]