Preprints
There are 2643 Preprints listed.
When homology fails: lessons from liver-fluke phylogenies
Published: 2025-06-12
Subjects: Life Sciences
Misaligned sequences derail evolutionary inference. Datasets from GenBank require verification of positional homology and orientation before alignment and phylogenetic analysis. Liver-fluke case studies reveal how overlooked errors skew results, underscoring the need for rigorous checks in parasitology and all molecular research.
Mapping disturbance across California’s rapidly changing forests
Published: 2025-06-12
Subjects: Environmental Monitoring, Natural Resources and Conservation, Natural Resources Management and Policy, Sustainability
Disturbances shape assemblages and spatial patterns of flora and fauna across the globe, and accurate disturbance mapping can aid conservation science and decision-making. However, mapping and differentiating among disturbance types using remote sensing is challenging, especially in forests with hidden subcanopy disturbances. On federal lands in the western US, wildfire, drought, and fuels [...]
National-scale datasets systematically underestimate vegetation recovery in Australian carbon farming projects
Published: 2025-06-11
Subjects: Environmental Monitoring
Limiting global warming below 2C requires nature-based climate solutions which are expected to supply more than a third of cost-effective climate mitigation by 2030, while prioritising avoiding fossil fuel emissions. Regenerating native forests under the Australian Government’s Australian Carbon Credit Unit (ACCU) program are delivering large-scale carbon storage across approximately 3.4 million [...]
Non-native grass invasion drives biodiversity loss after a single fire in a semi-arid shrubland
Published: 2025-06-10
Subjects: Biodiversity, Desert Ecology, Terrestrial and Aquatic Ecology
In the United States Great Basin, invasive annual grasses have initiated a novel grass-fire cycle that has transformed vast areas of semi-arid shrublands into non-native annual grasslands that now burn frequently. After the initial transformation, the system is so fire prone that it is difficult to find areas that have not burned repeatedly. We evaluated how the ecosystem responds in the absence [...]
A draft genome assembly for the dart-poison frog Phyllobates terribilis
Published: 2025-06-10
Subjects: Biology, Genomics
Dendrobatid poison frogs have become well established as model systems in several fields of biology. Nevertheless, the development of molecular and genetic resources for these frogs has been hindered by their large, highly repetitive genomes, which have proven difficult to assemble. Here we present a draft assembly for Phyllobates terribilis (12.6Gb), generated using a combination of sequencing [...]
Marine Conservation Leadership: Does Australia Walk the Talk?
Published: 2025-06-10
Subjects: Natural Resources and Conservation
The conservation of Australia's extraordinary marine biodiversity has been prominently championed over the past three decades by successive Federal and State Governments, who have consistently portrayed the nation as a global leader in marine protection. Here, we question whether this reputation is justified. We highlight substantial—and in some cases catastrophic—declines in marine species and [...]
Colorful birds face heightened extinction risk around the world
Published: 2025-06-10
Subjects: Biodiversity, Ecology and Evolutionary Biology, Environmental Studies, Life Sciences, Ornithology
Many of the functional traits that mediate extinction risk across the tree life relate indirectly to a species’ ability to persist in a changing world. Yet, there are certain traits such as coloration that directly affect human interactions with wildlife. Here, we use an existing dataset of color metrics for 4334 passerine bird species combined with global functional trait data to determine [...]
The interplay between ecological networks drives host-plasmid community dynamics
Published: 2025-06-10
Subjects: Ecology and Evolutionary Biology, Life Sciences
Plasmids drive bacterial evolution by transferring adaptive traits, such as metabolism and antimicrobial resistance, between members of microbial communities. There are trends and barriers to plasmid transmission, which likely emerge from both host-plasmid interactions (e.g. plasmid host ranges), and plasmid-plasmid interactions (e.g. plasmid incompatibility). However, while both interaction [...]
Delayed Reproduction as a Driver of Longevity
Published: 2025-06-10
Subjects: Life Sciences
Understanding the evolutionary forces that shape aging is central to both biology and medicine. While classic theories—such as Medawar’s mutation accumulation, Williams’ antagonistic pleiotropy, and Kirkwood’s disposable soma—have provided foundational insights, the population-level consequences of reproductive timing remain underexplored. Here, I propose that delayed reproduction may intensify [...]
Generalized graphical mixed models connect ecological theory with widely used statistical models
Published: 2025-06-06
Subjects: Applied Statistics, Aquaculture and Fisheries Life Sciences, Biodiversity, Environmental Sciences, Multivariate Analysis
Ecological dynamics are analyzed across multiple sites, times, and variables. Here, we introduce the family of generalized graphical mixed models (GGMMs) and show that it extends structural equation, generalized additive, and generalized linear mixed models. GGMMs represent ecological systems using a mathematical graph, where each analytic unit (node for each site-time-variable) has a direct [...]
Best practices for moving from correlation to causation in ecological research
Published: 2025-06-06
Subjects: Applied Statistics, Longitudinal Data Analysis and Time Series, Other Ecology and Evolutionary Biology, Statistical Methodology
In ecology, causal questions are ubiquitous, yet the literature describing systematic approaches to answering these questions is vast and fragmented across different traditions (e.g., randomization, structural equation modeling, convergent cross mapping). In our Perspective, we connect the causal assumptions, tasks, frameworks, and methods across these traditions, thereby providing a synthesis of [...]
Subterranean environments contribute to three-quarters of classified ecosystem services
Published: 2025-06-06
Subjects: Life Sciences
Beneath the Earth’s surface lies a network of interconnected caves, voids, and systems of fissures forming in rocks of sedimentary, igneous, or metamorphic origin. Though largely inaccessible to humans, this hidden realm supports and regulates services critical to ecological health and human well-being. Subterranean ecosystems are integral to major biogeochemical cycles, sustain diverse surface [...]
Evolution of Nassauvia Comm. ex Juss. (Asteraceae; Nassauvieae): new insights from old data
Published: 2025-06-06
Subjects: Biodiversity
The present work collated and reanalyzed DNA sequences for species of Nassauvia Comm. ex Juss. (including erstwhile Triptilion Ruiz & Pav.) (Asteraceae, Nassauvieae) reported in several previously published phylogenetic analyses. These sequences included: (i) the nuclear ribosomal DNA internal transcribed spacer region (ITS) and (ii) 5’ external transcribed spacer (ETS), and (iii) the [...]
Withdrawn: Which phenotypic traits are under selection under warm, dry climates in black spruce?
Published: 2025-06-06
Subjects: Life Sciences
• Trees are increasingly at risk of maladaptation to their environment as climates change rapidly world-wide. Although adaptive evolution by natural selection is a key mechanism by which populations and species can avoid extinction in changing environments, we have limited information regarding the phenotypic traits under selection under warm and dry environments. We answer the following research [...]
Systematic mapping and bibliometric analysis of meta-analyses on animal cognition
Published: 2025-06-03
Subjects: Ecology and Evolutionary Biology, Psychiatry and Psychology
Meta-analyses play an important role in empirically synthesising research and guiding future directions. The field of animal cognition is rapidly expanding, with both empirical and review papers increasing at a faster rate than those in the life sciences overall. However, the use of meta-analyses, their methodological rigour, and the geographic distribution of research activity remain unclear. We [...]