Preprints
Filtering by Subject: Behavior and Ethology
Gradual development and chance beget individuality
Published: 2026-06-02
Subjects: Behavior and Ethology, Developmental Biology, Ecology and Evolutionary Biology, Evolution
Behaviors – and thus behavioral individuality – rarely emerge fully formed but are instead built gradually through development, shaped by processes involving learning, skill formation, and experience. Prevailing theory in behavioral ecology, however, has largely focused on static equilibrium outcomes where behaviors are analyzed only as fully formed traits, often neglecting development. Here, we [...]
Evaluating the potential of molecular dietary analysis of predators for the detection of emerging plant pests
Published: 2026-06-01
Subjects: Agriculture, Behavior and Ethology, Biodiversity, Biosecurity, Ecology and Evolutionary Biology, Entomology, Forest Biology, Forest Management, Forest Sciences, Genetics, Laboratory and Basic Science Research Life Sciences, Life Sciences, Research Methods in Life Sciences
Monitoring plant pests is crucial for maximising yields across agricultural and forest production systems, but also for the mitigation of invasive species spread. Traditional monitoring methods, such as mass trapping and direct observation, scale poorly and introduce latency between collection, detection and response. Since many plant pests are frequently consumed by predators, molecular dietary [...]
The coevolution of cooperation and socially-mediated dispersal: a model
Published: 2026-06-01
Subjects: Behavior and Ethology, Evolution, Population Biology
Limited dispersal can promote the evolution of cooperation by increasing relatedness between social partners. However it also intensifies kin competition, potentially cancelling the benefits of helping. Here, we analyse a model in which individuals evolve both (i) the probability of cooperating within social groups as adults, and (ii) the dispersal probability of juveniles conditional on the [...]
Methodological choices influence ecological inference in passive acoustic monitoring of a Neotropical nightjar
Published: 2026-05-29
Subjects: Behavior and Ethology, Biodiversity, Population Biology
Passive acoustic monitoring is increasingly used to investigate species activity and habitat use through occupancy analyses. Yet, the complex analytical workflow, from automated detector choice to confidence thresholds and statistical modeling framework, is amongst the factors that influence ecological inference, and the extent these decisions affect modelling outputs is poorly debated. Here, we [...]
Expanding the sentinel approach through multimodal integration: resolving underlying ecological processes with eDNA and computer vision
Published: 2026-05-19
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Life Sciences, Other Ecology and Evolutionary Biology, Research Methods in Life Sciences, Terrestrial and Aquatic Ecology
Sentinel approaches provide a semi-controlled method for quantifying in-field ecological interactions and processes while reducing bias and labour. They are, however, limited by difficulties ascribing taxonomic identities, behavioural context and temporal resolution to interacting agents. The integration of additional sources of data, including the analysis of DNA left behind on sentinel objects [...]
Sex differential effects of developmental heat stress on life-history and reproductive traits
Published: 2026-05-01
Subjects: Behavior and Ethology, Biology, Ecology and Evolutionary Biology, Evolution, Life Sciences
Global warming has led to increased mean global temperatures with projections suggesting continued warming throughout this century, posing an escalating threat to biological systems worldwide. Ectotherms are most vulnerable to this change as heat stress conditions can have severe implications on their development, mating interactions, and fitness. However, the sex-specific effects of [...]
Extending {spatsoc} to measure intragroup social dynamics
Published: 2026-05-01
Subjects: Animal Studies, Behavior and Ethology, Ecology and Evolutionary Biology, Software Engineering
Beyond proximity-based social networks and home range overlap, animal telemetry data can also be used to measure intragroup social dynamics including individual position within groups, individual and group level movement directions, leadership patterns and lagged follower behaviours. We used a scoping review of literature across domains, including behavioural ecology, collective movement, and [...]
A Substrate-Driven Plasticity Hypothesis for the Monterey Ensatina Salamander (Ensatina eschscholtzii)
Published: 2026-04-30
Subjects: Behavior and Ethology
Abstract.—Phenotypic plasticity allows many amphibians, including several salamander species, to adjust skin luminance in response to background brightness. In habitats with heterogeneous substrates in color and brightness, such plasticity may generate substantial individual variation within a population. In a population of the terrestrial salamander Ensatina eschscholtzii, a recent study [...]
Reindeer habitat selection and movement changes with cumulative impacts from mining and wind power development
Published: 2026-04-30
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Forest Management, Life Sciences, Terrestrial and Aquatic Ecology, Zoology
Industrial expansion often occurs in landscapes already affected by multiple disturbances, leading to cumulative impacts on biodiversity and local communities. With the societal pressure for an rapid ‘green transition’, land-use changes intensifies, yet most impact assessments remain local and project-based, disregarding cumulative impacts. We conceptualized and disentangled key dimensions of [...]
Plastic shifts in thermal preference and thermoregulation strategy across ontogeny in an invasive fly
Published: 2026-04-21
Subjects: Animal Sciences, Behavior and Ethology, Biology, Ecology and Evolutionary Biology, Entomology, Life Sciences, Other Ecology and Evolutionary Biology, Zoology
Behavioural thermoregulation allows ectotherms to escape extreme or seek optimal temperatures. Its precision can impact survival and fitness under changing conditions and its plasticity can be an adaptive strategy when the plasticity of thermal limits is insufficient to buffer against warming. We explore the developmental and intergenerational plasticity of behavioural thermoregulation strategies [...]
Acute bacterial challenge in Drosophila reveals age and sex dependent feeding and macronutrient choice without generalised anorexia
Published: 2026-04-10
Subjects: Behavior and Ethology, Entomology, Integrative Biology, Zoology
Sickness behaviours are often interpreted as adaptive host responses that reallocate resources from performance to defence. Anorexia - a reduction in food intake - is one of the most frequently cited examples, yet evidence across insects is variable and rarely separates the effects of wounding, immune stimulation, and live infection. Here we use a factorial design in Drosophila melanogaster to [...]
Why are embodied social signals concentrated towards the rostral region? — The rostrum concentration hypothesis
Published: 2026-04-09
Subjects: Behavior and Ethology, Biological Psychology, Ecology and Evolutionary Biology, Life Sciences, Other Ecology and Evolutionary Biology, Psychology, Social and Behavioral Sciences
Although frequently embodied, the relationship of animal social communication with body layout has rarely been investigated from a unified cognitive perspective. Across animal taxa, socially relevant signals, ranging from facial expressions and gaze to colouration and morphology, are strikingly concentrated towards the anterior region of the body. Here, we propose the Rostrum Concentration [...]
Coexistence of phenotypic plasticity and habitat use in natural populations
Published: 2026-03-23
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Evolution, Life Sciences
When studying how individuals adapt to environmental changes, the environment is traditionally viewed as a passive backdrop, with individuals modifying their phenotype in response to environmental conditions (i.e., phenotypic plasticity). However, this perspective overlooks the active role of habitat choice in mediating individual responses to environmental changes. In this paper, we argue for [...]
Genetic variance and phenotypic selection on pathogen-linked oviposition choice in Drosophila
Published: 2026-03-23
Subjects: Animal Experimentation and Research, Behavior and Ethology, Entomology, Evolution, Genetics, Integrative Biology, Research Methods in Life Sciences, Zoology
Pathogen-avoidance behaviour is assumed to be adaptive, yet its phenotypic variability and genetic heritability are rarely quantified. In species lacking post-oviposition care, avoiding potentially infectious egg-laying substrates would improve offspring survival and should therefore be under strong selection. We used two-choice oviposition assays to quantify the phenotypic and genetic variance [...]
Heritabilities and genetic correlations of Drosophila melanogaster locomotory behaviour traits: a high-throughput phenotyping approach
Published: 2026-03-23
Subjects: Behavior and Ethology, Evolution
Genetic variance forms the basis for evolutionary inferences as it describes the evolutionary potential of traits. The major limitation of quantitative genetic studies is achieving sufficient power and sample sizes to estimate heritabilities with sufficient precision. This issue is especially important in the case of traits that are inherently susceptible to stochastic, nonbiological variation. [...]