Preprints
Filtering by Subject: Evolution
Lactate Dehydrogenase as a Candidate Genomic Marker of Climate Change in Mammals?
Published: 2026-07-13
Subjects: Bioinformatics, Comparative and Evolutionary Physiology, Evolution, Genetics, Genomics, Molecular Genetics, Other Ecology and Evolutionary Biology, Population Biology
Climate change imposes metabolic and thermal stress on mammals, yet genomic markers that track lineage specific adaptation remain limited. Lactate dehydrogenase (LDH), a central enzyme in lactate metabolism and anaerobic stress response, has not previously been evaluated for its evolutionary association with climate change induced selection. Here, comparative genomics across 14 mammalian species [...]
RSV G selection analyses support constraint of the CX3C/cystine-noose core and diversification in mucin-like regions
Published: 2026-07-07
Subjects: Evolution
The RSV attachment (G) glycoprotein is a highly variable surface antigen and an important target of humoral immunity, yet it contains a short central conserved region (CCR; RSV-A2 residues 157-198) with a CX3C chemokine-mimic motif and disulfide-bonded cystine noose. A pre-specified hypothesis was tested: FEL-positive diversifying sites are enriched in the CCR and/or the CX3C motif. Using a [...]
Genome-wide strengthening of evolutionary constraint across the volvocine multicellularity gradient
Published: 2026-07-03
Subjects: Computational Biology, Ecology and Evolutionary Biology, Evolution, Genetics and Genomics, Genomics
The evolutionary cost of maintaining somatic cell populations has been hypothesised to drive proteome-wide strengthening of constraint on coding sequence at the origin of multicellularity. Prior work on the volvocine clade has reported lower d_N/d_S in colonial than in unicellular relatives on 55 chloroplast genes (Hu et al. 2019) and on 105 nuclear single-copy orthogroups across colonial species [...]
A self tuning sliding window method for detecting phenotype linked regional poly-methylation architecture in sparse wildlife methylomes
Published: 2026-06-26
Subjects: Bioinformatics, Cellular and Molecular Physiology, Comparative and Evolutionary Physiology, Evolution, Genetics and Genomics, Marine Biology, Other Ecology and Evolutionary Biology
Despite featuring extreme physiological adaptations integration of wildlife species into the modern ‘omics’ frameworks are limited due to the sparsity in the data. To address the sparsity limitation a self-tuning sliding-window framework was developed for the identification of the regional poly-CpG methylation architecture associated with phenotypic traits. Under the framework the iteratively [...]
Beyond mistakes: same-sex partner acceptance and broad mating filters coexist in termite pairing
Published: 2026-06-24
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Entomology, Evolution
Same-sex sexual behavior is often interpreted either as a mistake arising from indiscriminate attempts or as an adaptive behavior directed towards same-sex partners. These explanations are typically considered mutually exclusive. Here we challenge this assumption using an adaptive same-sex pairing system in Reticulitermes termites. Long-term male-male pairings originate from tandem running, in [...]
A mathematical foundation of modelling thermal injury and repair dynamics in ectotherms
Published: 2026-06-18
Subjects: Animal Experimentation and Research, Ecology and Evolutionary Biology, Environmental Indicators and Impact Assessment, Environmental Sciences, Evolution, Life Sciences, Physiology, Plant Sciences, Population Biology, Research Methods in Life Sciences, Statistical Models, Statistics and Probability, Survival Analysis
As global temperatures rise and extreme heat events impair ectotherm performance and survival, it is becoming increasingly important to predict how organisms accumulate and repair thermal injury under realistic benign and stressful temperatures. The thermal death time (TDT) model quantifies how heat events translate into thermal injury, but under natural temperature fluctuations the TDT model is [...]
Saved by the Symbiont: Environmental Stress Intensity and Endosymbiont-Mediated Stress Response Determine Evolved Host Complexity
Published: 2026-06-17
Subjects: Evolution, Life Sciences
Understanding how stress responses affect the trajectory of host–symbiont coevolution is central to predicting and managing species outcomes in the face of disturbances to ecosystems. Critically, it remains an open question how exactly we expect stressors to influence the coevolutionary dynamics of symbioses (on either end of the parasitism–mutualism continuum). In this work, we use in silico [...]
An integrated framework for unifying our understanding of nonconsumptive predation risk effects
Published: 2026-06-12
Subjects: Behavior and Ethology, Biology, Ecology and Evolutionary Biology, Evolution, Integrative Biology, Life Sciences, Other Ecology and Evolutionary Biology, Population Biology, Terrestrial and Aquatic Ecology
Predation risk can induce risk-induced trait responses (RITRs) – changes in prey defensive traits including behavior, morphology, life history, and physiology – thought to have profound effects on prey fitness and population dynamics (termed ‘nonconsumptive effects’). Yet, predicting the magnitude of RITRs and their fitness consequences remains difficult because outcomes depend heavily on [...]
Gradual development and chance beget individuality
Published: 2026-06-02
Subjects: Behavior and Ethology, Developmental Biology, Ecology and Evolutionary Biology, Evolution
Behaviors – and thus behavioral individuality – rarely emerge fully formed but are instead built gradually through development, shaped by processes involving learning, skill formation, and experience. Prevailing theory in behavioral ecology, however, has largely focused on static equilibrium outcomes where behaviors are analyzed only as fully formed traits, often neglecting development. Here, we [...]
The coevolution of cooperation and socially-mediated dispersal: a model
Published: 2026-06-01
Subjects: Behavior and Ethology, Evolution, Population Biology
Limited dispersal can promote the evolution of cooperation by increasing relatedness between social partners. However it also intensifies kin competition, potentially cancelling the benefits of helping. Here, we analyse a model in which individuals evolve both (i) the probability of cooperating within social groups as adults, and (ii) the dispersal probability of juveniles conditional on the [...]
The holobiont is not a useful model for most host-microbiome interactions
Published: 2026-05-20
Subjects: Environmental Microbiology and Microbial Ecology Life Sciences, Evolution, Life Sciences
The holobiont concept refers to a host and associated microbes. It has been critiqued over the last decade, primarily based on the argument that individual holobionts are not an appropriate level for analyzing multi-generation host dynamics, as most microbes are acquired from the environment. Several responses were given to this and other criticisms. The main response has been that the holobiont [...]
Summarizing Populations: Characterizing the Effects of Sampling in Computational Evolutionary Replay Experiments
Published: 2026-05-19
Subjects: Evolution
When we sample an evolving population, how well do we capture its long-term evolutionary potential? This question underlies the validity of analytical replay experiments, which restart evolution from multiple points in a population’s history to measure how long-term potential changed over time. Analytical replay experiments are becoming increasingly popular in both wet-lab and computational [...]
The bacterial immune system: identifying evolved defense adaptations
Published: 2026-05-13
Subjects: Ecology and Evolutionary Biology, Evolution, Microbiology
The last few years have witnessed a rapid expansion of reported bacterial defense mechanisms. Alongside established mechanisms of defense against molecular parasites (e.g. CRISPR-Cas, restriction-modification), hundreds of novel defenses are being described each year, contributing to an ever-expanding ‘bacterial immune system’. Terms like ‘defense’ and ‘immune’ are often used as shorthand for an [...]
Sexually antagonistic selection: a review of the theory and its implications
Published: 2026-05-08
Subjects: Ecology and Evolutionary Biology, Evolution, Population Biology
Sexually antagonistic selection arises when females and males have different fitness optima for traits with a shared genetic basis, so that the same alleles are favoured in one sex but disfavoured in the other. It has been implicated in a wide range of ecological and evolutionary processes, from the maintenance of a sex load to the evolution of sex chromosomes. Mathematical models have long been [...]
The evolutionary link between food, condiments and medicine
Published: 2026-05-06
Subjects: Agricultural Science, Anthropology, Biodiversity, Biology, Botany, Ecology and Evolutionary Biology, Evolution, Food Science, Life Sciences, Plant Biology, Plant Sciences, Social and Behavioral Sciences
The deep relationship between humans and plants is of great interest to ethnobotanists, human ecologists, and evolutionary biologists. Humans have incorporated thousands of plant species into both traditional medicine and our diets, as foods and condiments. Many of these provide not only calories but also micronutrients and other bioactive compounds that contribute to health [1]. The boundaries [...]