Nicholas C. Wu , Lesley Ann Alton, Rafael P Bovo, Nicholas Carey, Shannon E Currie, John R B Lighton, Andrew E McKechnie, Patrice Pottier , Giulia Rossi, Craig R White, Danielle Levesque

Respirometry is an important tool for understanding whole-animal energy and water balance in relation to their environment. Consequently, the growing number of studies using respirometry over the last decade warrants reliable reporting and data sharing for effective dissemination and research synthesis. We provide a checklist guideline on five key areas to facilitate the transparency and reproducibility of respirometry studies: 1) materials, set up, plumbing, 2) subject conditions/maintenance, 3) measurement conditions, 4) data processing, and 5) data reporting and statistics, each with explanations and example studies. Transparency in reporting and data availability has benefits on multiple fronts. Authors can use this checklist to design and report on their study, and reviewers and editors can use the checklist to assess the reporting quality of the manuscripts they review. Improved standards for reporting will enhance the value of primary studies and will greatly facilitate the ability to carry out higher quality research syntheses to address ecological and evolutionary theories.