Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations

This is a Preprint and has not been peer reviewed. The published version of this Preprint is available: This is version 2 of this Preprint.


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Miriam Merenciano, Laura Aguilera, Josefa Gonzalez


This protocol generates a precise deletion of a transposable element (TE) in a natural population of Drosophila melanogaster using two steps of CRISPR-Cas9 homology-directed repair. In the first step, the TE is replaced by a fluorescent marker, while in the second CRISPR-Cas9 step the fluorescence marker is removed to avoid any possible effect of the introduced marker sequence. This two-step protocol thus produces a precise deletion of any genomic region, exemplified here with a TE, while facilitating the screening of positive CRISPR-Cas9 events in natural populations without altering their genetic background.
For complete details on the use and execution of this protocol, please refer to (Merenciano & Gonzalez, 2023).



Life Sciences


CRISPR/Cas9, Drosophila, natural population


Published: 2023-02-15 16:56

Last Updated: 2023-02-15 21:56

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CC-By Attribution-NonCommercial-NoDerivatives 4.0 International

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