DNA methylation patterns in the round goby hypothalamus support an on-the-spot decision scenario for territorial behaviour

This is a Preprint and has not been peer reviewed. The published version of this Preprint is available: https://doi.org/10.3390/genes10010004. This is version 3 of this Preprint.

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Authors

Irene Adrian-Kalchhauser, Vincent Somerville, Michaela Schwaiger, Philipp Hirsch, Karen Bussmann, Isabelle C. Gebhardt, Jean-Claude Walser, Patricia Burkhardt-Holm, Alexandra Weyrich

Abstract

How early life experiences are stored on a molecular level and affect behavioural phenotypes later in life is not well understood. In fish, reproductive phenotypes are often easily discernible and frequently depend on previous life experiences. DNA methylation is an epigenetic mechanism which is both sensitive to environmental conditions and stable across cell divisions. In this study, we therefore investigate whether DNA methylation mediates early life experiences and predetermines the territorial male reproductive phenotype in the round goby, Neogobius melanostomus. We investigate early life predisposition by growth back-calculations and then study DNA methylation by MBD-Seq in the round goby hypothalamus as the brain region controlling vertebrate reproductive behaviour. We find that the territorial reproductive phenotype is linked to a high growth rate in the first year of life. Hypothalamic DNA methylation patterns, however, reflect the current behavioural status independently of early life experiences. Together, our data suggest a non-predetermination scenario in which indeterminate males progress to a non-territorial status in the spawning season, and in which some males then assume a specialized territorial phenotype if current conditions are favourable.

DOI

https://doi.org/10.32942/osf.io/teuhb

Subjects

Genetics and Genomics, Genomics, Life Sciences

Keywords

brain, epigenetic mechanisms, latent effects, Neogobius melanostomus, reproductive strategy

Dates

Published: 2018-12-11 04:10

Last Updated: 2019-01-21 08:18

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CC-By Attribution-ShareAlike 4.0 International