Complete de novo assembly of Wolbachia endosymbiont of contemporary Drosophila simulans using long-read genome sequencing.

Jodie Jacobs, Alexandra Lum, Elyse Mina, Camryn N. Morey, Darren D. Lee, Emry L Gutierrez, Jonah A Dionisio, Cade Mirchandani, Luke Sylvester, Anne Nakamoto, Hailey Loucks, Ciara Wanket, Ariana Cisneros, Alessandro Calicchio, Alexis Enstrom, Camille Headrick, Faith Okamoto, Harrison D Heath, Kseniya Malukhina, Petria Russell, Sagorika Nag, Thomas Gillespie, William Sobolewski, Zia Truong, Shelbi L. Russel

We present a contemporary high-quality, complete de novo assembly of Wolbachia pipientis (wRi Merrill 23, CP199298), an alphaproteobacterial endosymbiont of Drosophila simulans. This assembly was generated using long read sequencing of wRi-infected D. simulans embryos collected from the Merrill College at the University of California, Santa Cruz in October 2023.