Complete de novo assembly of Wolbachia endosymbiont of contemporary Drosophila simulans using long-read genome sequencing.

Complete de novo assembly of Wolbachia endosymbiont of contemporary Drosophila simulans using long-read genome sequencing.

This is a Preprint and has not been peer reviewed. This is version 1 of this Preprint.

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Authors

Jodie Jacobs, Alexandra Lum, Darren D. Lee, Emry L Gutierrez, Jonah A Dionisio, Camryn N. Morey, Cade Mirchandani, Luke Sylvester, Hailey Loucks, Anne Nakamoto, Ciara Wanket, Ariana Cisneros, Alessandro Calicchio, Alexis Enstrom, Camille Headrick, Faith Okamoto, Kseniya Malukhina, Harrison D Heath, Petria Russell, Sagorika Nag, Thomas Gillespie, William Sobolewski, Zia Truong, Shelbi L. Russel

Abstract

We present a contemporary high-quality, complete de novo assembly of Wolbachia pipientis (wRi Merrill 23, CP199298), an alphaproteobacterial endosymbiont of Drosophila simulans. This assembly was generated using long read sequencing of wRi-infected D. simulans embryos collected from the Merrill College at the University of California, Santa Cruz in October 2023.

DOI

https://doi.org/10.32942/X2BM0X

Subjects

Evolution, Genomics

Keywords

Wolbachia, Drosophila simulans, Symbiosis

Dates

Published: 2025-10-09 06:58

Last Updated: 2025-10-09 06:58

License

No Creative Commons license

Additional Metadata

Language:
English

Data and Code Availability Statement:
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1312834/, https://github.com/jodiejacobs/Jacobs_et_al_2026_de_novo_wRi_merrill_23_assembly.