Preprints
Filtering by Subject: Other Genetics and Genomics
Measuring natural selection on the transcriptome
Published: 2025-03-11
Subjects: Ecology and Evolutionary Biology, Evolution, Other Genetics and Genomics
The level and pattern of gene expression is increasingly recognized as a principal determinant of plant phenotypes and thus of fitness. The estimation of natural selection on the transcriptome is an emerging research discipline. We here review recent progress and consider the challenges posed by the high dimensionality of the transcriptome for the multiple regression methods routinely used to [...]
Transferable approaches to CRISPR-Cas9 induced genome editing in non-model insects: a brief guide
Published: 2025-02-27
Subjects: Animal Experimentation and Research, Animal Sciences, Entomology, Genetics, Life Sciences, Molecular Genetics, Other Genetics and Genomics, Zoology
Despite the large variety of insect species with divergent morphological, developmental and physiological features questions on gene function could for a long time only be addressed in few model species. The adoption of the bacterial CRISPR-Cas system for genome editing in eukaryotic cells widened the scope of the field of functional genetics: for the first time the creation of heritable genetic [...]
Combining eDNA and Museomics to Enhance Biodiversity Monitoring
Published: 2025-02-25
Subjects: Biodiversity, Life Sciences, Other Ecology and Evolutionary Biology, Other Genetics and Genomics
Biodiversity changes due to human activities highlight the need for efficient biodiversity monitoring approaches. Environmental DNA (eDNA) metabarcoding offers a non-invasive method to assess species distributions, but its accuracy depends on comprehensive DNA reference databases. Natural history museum collections often contain rare or difficult to obtain samples that could be used as a resource [...]
Comparing ecological and evolutionary variability within datasets
Published: 2020-01-28
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Evolution, Genetics and Genomics, Life Sciences, Other Ecology and Evolutionary Biology, Other Genetics and Genomics, Social and Behavioral Sciences
Many key questions in evolutionary ecology require the use of variance ratios such as heritability, repeatability, and individual resource specialization. These ratios allow to understand how phenotypic variation is structured into genetic and non-genetic components, to identify how much organisms vary in the resources they use or how functional traits structure species communities. Understanding [...]
Embedding indigenous principles in genomic research of culturally significant species: a conservation genomics case study
Published: 2019-09-30
Subjects: Genetics and Genomics, Life Sciences, Other Genetics and Genomics
Indigenous peoples around the world are leading discussion regarding genomic research of humans, and more recently, species of cultural significance, to ensure the ethical and equitable use of DNA. Within a Māori (indigenous people of Aotearoa New Zealand) worldview, genomic data obtained from taonga (culturally significant) species has whakapapa – generally defined as genealogy, whakapapa layers [...]