Preprints
Filtering by Subject: Research Methods in Life Sciences
Genetic variance and phenotypic selection on pathogen-linked oviposition choice in Drosophila
Published: 2026-03-23
Subjects: Animal Experimentation and Research, Behavior and Ethology, Entomology, Evolution, Genetics, Integrative Biology, Research Methods in Life Sciences, Zoology
Pathogen-avoidance behaviour is assumed to be adaptive, yet its phenotypic variability and genetic heritability are rarely quantified. In species lacking post-oviposition care, avoiding potentially infectious egg-laying substrates would improve offspring survival and should therefore be under strong selection. We used two-choice oviposition assays to quantify the phenotypic and genetic variance [...]
Insect oviposition as a simple system to investigate the ecology and evolution of pathogen avoidance behaviour
Published: 2026-03-20
Subjects: Animal Experimentation and Research, Behavior and Ethology, Biology, Entomology, Evolution, Immunology and Infectious Disease, Integrative Biology, Laboratory and Basic Science Research Life Sciences, Research Methods in Life Sciences, Zoology
Behavioural avoidance of pathogens and parasites is a ubiquitous first line of defence, yet we lack tractable systems that connect cue detection to fitness consequences, population transmission, and coevolution. We propose insect oviposition as a model that yields general principles for avoidance across taxa. Oviposition decisions fix offspring exposure, they are governed by well‑mapped sensory [...]
A comparison of methods to assess selective disappearance and quantify ageing
Published: 2026-02-25
Subjects: Ecology and Evolutionary Biology, Research Methods in Life Sciences
1. Age-dependent change in traits at the population-level level can diverge from the within-individual ageing trajectory due to selective disappearance, the biased removal or death of individuals with certain phenotypes. 2. Commonly used methods to assess, and account for, selective disappearance have been developed for relatively simple settings. As such, we currently lack a clear understanding [...]
Composite virulence: useful metric or conceptual trap?
Published: 2026-02-20
Subjects: Animal Diseases, Animal Experimentation and Research, Animal Sciences, Biodiversity, Biology, Ecology and Evolutionary Biology, Evolution, Immunity, Immunology and Infectious Disease, Immunology of Infectious Disease, Immunopathology, Life Sciences, Medical Microbiology, Microbiology, Other Ecology and Evolutionary Biology, Other Immunology and Infectious Disease, Parasitic Diseases, Parasitology, Pathogenic Microbiology, Plant Pathology, Research Methods in Life Sciences, Zoology
Virulence, the harm an infection causes to its host, is a cornerstone concept in ecology and evolution, yet it remains difficult to quantify because infection impact is multidimensional, dynamic, and context-dependent. Infections can reduce host performance through multiple, partially redundant routes (including mortality, fecundity loss, behavioural impairment, and physiological disruption), [...]
One Toolbox, Many Tools: A Practitioner’s Guide to Latent Variable Modelling for Community Ecology
Published: 2026-02-05
Subjects: Ecology and Evolutionary Biology, Multivariate Analysis, Research Methods in Life Sciences, Statistical Methodology, Statistical Models
In this article, we present the case for Generalized Linear Latent Variable Models (GLLVMs) as a go-to choice of statistical method for any community ecologist wanting to tackle a range of present-day ecological research questions. GLLVMs bring tools and capabilities from classic (mixed-effects) regression models to multivariate community analysis, providing a number of novel ways to tailor [...]
Shared acoustic manifolds for exploratory comparison of passerine vocalizations
Published: 2026-01-23
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Research Methods in Life Sciences
This study presents a fixed-parameter pipeline designed to support reproducible embedding of frame-level representations of multiple passerine vocalizations within shared low-dimensional spaces. Three passerine species are considered: Eurasian Wren, Tree Pipit and Common Chaffinch, with a selection of four individuals for each species group. Vocalization frames from each species group are mapped [...]
Phenotyping avian bill sizes; combining the collection of standardized still images with software to obtain observer-independent measures of avian bill shapes
Published: 2026-01-02
Subjects: Animal Sciences, Ecology and Evolutionary Biology, Life Sciences, Research Methods in Life Sciences
Avian bill size is a morphological trait with evolutionary and ecological importance. Obtaining large-scale observer-independent measures of bill length and bill depth has proven to be challenging. We developed a device, the Bill Phenotyping Box, that allows taking standardized still images from wild small passerine birds in the field. We combine this with dedicated software that, based on a [...]
sitetool: an application for field site selection and evaluation
Published: 2025-10-27
Subjects: Life Sciences, Research Methods in Life Sciences
Field studies are fundamental to ecological research, yet many studies rely on unspecified or convenience-based methods for site selection, potentially introducing bias that can compromise research results. Remote-sensing data provides a quantitative way to evaluate potential sites without expensive pilot visits, however, interacting with spatial data can be computationally complex. We present an [...]
GhostNetZero: AI for Detecting Marine Ghost Nets
Published: 2025-09-23
Subjects: Artificial Intelligence and Robotics, Biodiversity, Databases and Information Systems, Environmental Monitoring, Marine Biology, Research Methods in Life Sciences, Sustainability
Abandoned, lost, or otherwise discarded fishing gears (ALDFG), commonly referred to as ghost nets, pose a persistent global threat to marine biodiversity. Constructed from durable synthetic polymers, ghost nets remain intact for decades, continuing to entangle and kill marine organisms while damaging habitats and imposing economic burdens on fisheries and coastal communities. Despite their [...]
The myth of the metabolic baseline: how sleep-wake cycles undermine a foundational assumption in organismal biology
Published: 2025-08-28
Subjects: Animal Experimentation and Research, Biochemistry, Biophysics, and Structural Biology, Biology, Comparative and Evolutionary Physiology, Life Sciences, Physiology, Research Methods in Life Sciences, Zoology
Basal and standard metabolic rates (BMR and SMR) are cornerstones of physiological ecology and are assumed to be relatively fixed intrinsic properties of organisms that represent the minimum energy required to sustain life. However, this assumption is conceptually flawed. Many core maintenance processes underlying SMR are temporally partitioned across sleep and wakefulness and are not [...]
“But I can’t preregister my research”: Improving the reproducibility and transparency of ecology and conservation with adaptive preregistration for model-based research
Published: 2025-08-19
Subjects: Ecology and Evolutionary Biology, Research Methods in Life Sciences
1. Preregistration is an open-science practice which aims to improve research transparency and mitigate questionable research practices, like cherry-picking results. It helps protect against cognitive biases, like hindsight bias, that can influence how study outcomes are interpreted. There has been little uptake of preregistration in ecology and conservation, arguably because existing [...]
movetrack: An R package to model flight paths from radio-telemetry networks
Published: 2025-07-04
Subjects: Research Methods in Life Sciences
Tracking small- to large-scale movements of animals is important for studying their interactions with the environment, including how they adjust and adapt their migration in response to environmental and human-induced changes. Despite the technical progress in tracking devices, a major challenge remains for small animals-such as songbirds, bats, and insects-because GPS transmitters are still too [...]
Going Global by Going Local: Impacts and Opportunities of Geographically Focused Data Integration
Published: 2025-06-17
Subjects: Biodiversity, Life Sciences, Research Methods in Life Sciences
Biodiversity conservation is a global challenge that requires the integration of global and local data. Expanding global data infrastructures have opened unprecedented opportunities for biodiversity data storage, curation, and dissemination. Within one such infrastructure – the Global Biodiversity Information Facility (GBIF) – these benefits are achieved by aggregating data from over 100 regional [...]
Elevating the importance of Risk of Bias assessment for ecology and evolution
Published: 2025-06-16
Subjects: Agriculture, Animal Sciences, Biodiversity, Biology, Ecology and Evolutionary Biology, Entomology, Life Sciences, Marine Biology, Microbiology, Plant Sciences, Research Methods in Life Sciences
Systematic reviews and meta-analyses are key evidence synthesis methods informing research and policy. An assessment of the Risk of Bias (RoB) in included studies is normally considered an essential component of these. However, RoB assessment is rare in ecology and evolutionary biology (EEB), and tools from other fields are seldom adopted. To identify reasons for this limited uptake, we surveyed [...]
Gendered male and high-income country authors dominate publication at a One Health research organization
Published: 2025-05-28
Subjects: Life Sciences, Publishing, Research Methods in Life Sciences, Social and Behavioral Sciences
Authorship on academic publications carries substantial weight for researchers in science fields. One’s position in a list of authors is typically used to signal information about author contributions and status, with the first and last authorship positions regarded as the most prestigious and important for career advancement. Therefore, any inequities that exist in the allocation of publication [...]