Preprints
Filtering by Subject: Genomics
Toxin resistance mechanisms span biological scales in the Royal Ground Snake (Colubridae: Erythrolamprus reginae)
Published: 2025-06-13
Subjects: Animal Sciences, Behavior and Ethology, Biology, Cellular and Molecular Physiology, Comparative and Evolutionary Physiology, Ecology and Evolutionary Biology, Evolution, Genomics, Integrative Biology, Life Sciences, Molecular Biology, Neuroscience and Neurobiology, Physiology, Systems and Integrative Physiology Life Sciences, Systems Biology, Zoology
Exposure to multiple toxic compounds imposes diverse selective pressures, potentially leading to a toxin-resistant phenotype that operates across biological levels. There are several known toxin resistance mechanisms–such as behavioral avoidance, metabolic detoxification, and target-site insensitivity. However, most studies have been conducted with exposure to a single toxin or have focused on [...]
Genomic data confirms that mutation cannot restore genetic diversity lost through population bottlenecks
Published: 2025-06-13
Subjects: Ecology and Evolutionary Biology, Genomics, Life Sciences, Molecular Genetics, Population Biology
Maintaining within-species genetic diversity is a critical goal of biodiversity conservation as it determines a species’ ability to adapt to environmental change. Without human intervention, isolated populations can only recover genetic diversity post-bottleneck through the accumulation of new mutations over evolutionary timescales. Using recent estimates of mutation rates from major genomic [...]
A draft genome assembly for the dart-poison frog Phyllobates terribilis
Published: 2025-06-10
Subjects: Biology, Genomics
Dendrobatid poison frogs have become well established as model systems in several fields of biology. Nevertheless, the development of molecular and genetic resources for these frogs has been hindered by their large, highly repetitive genomes, which have proven difficult to assemble. Here we present a draft assembly for Phyllobates terribilis (12.6Gb), generated using a combination of sequencing [...]
IQ2MC: A New Framework to Infer Phylogenetic Time Trees Using IQ-TREE 3 and MCMCTree with Mixture Models
Published: 2025-05-06
Subjects: Bioinformatics, Computational Biology, Ecology and Evolutionary Biology, Evolution, Genetics, Genetics and Genomics, Genomics, Life Sciences
IQ-TREE and MCMCTree are two widely used phylogenetic tools to infer phylogenetic trees and estimate divergence times, respectively. As MCMCTree performs fast approximate Markov Chain Monte Carlo sampling to obtain the times along a fixed tree topology, it would be natural to use IQ-TREE to obtain the tree. However, it is currently not possible to integrate these tools seamlessly, as MCMCTree [...]
Plant genomic variation and its implications for proposed EU NGT legislation
Published: 2025-04-09
Subjects: Biotechnology, Ecology and Evolutionary Biology, Evolution, Genetics, Genetics and Genomics, Genomics, Life Sciences, Molecular Genetics, Plant Breeding and Genetics Life Sciences, Plant Sciences
The European Commission proposal for New Genomic Techniques (NGTs) of July 2023 specifies that NGT1 plants, which are considered equivalent to conventional plants, may differ from the recipient or parental plant by no more than 20 insertions, which cannot be longer than 20 bp; deletions can be of any size and number. Here, we examine the proposed 20/20 NGT1 limit against the background of the [...]
De Novo Gene Emergence: Summary, Classification, and Challenges of Current Methods
Published: 2025-04-08
Subjects: Bioinformatics, Genomics
A novel mechanism of de novo gene origination from non-genic sequences was first proposed in the early 2000s. Subsequent studies have since provided evidence of de novo gene emergence across all domains of life, revealing its occurrence to be more frequent than initially anticipated. While studies mainly agree on the general concept of de novo emergence from non-genic DNA, the exact methods and [...]
Conservation macrogenetics reveals the potential hidden consequences of the 2019-2020 Black Summer fires on Australian biodiversity
Published: 2025-03-17
Subjects: Biodiversity, Genetics, Genomics, Life Sciences, Molecular Genetics, Other Ecology and Evolutionary Biology, Population Biology
The use of genetic analyses has become ubiquitous in conservation planning and management as biodiversity is increasingly threatened globally. Typically, such analyses are employed at the species-level, though as genetic data accrue, it is now possible to consider the genetic composition of multiple species across landscapes. Such macrogenetic perspectives can reveal the potential genetic [...]
Environmental RNA mitigates fish ghosts related to fish feeds for aquaculture in molecular ecological survey in a bay.
Published: 2024-12-19
Subjects: Aquaculture and Fisheries Life Sciences, Biodiversity, Genomics, Terrestrial and Aquatic Ecology
The use of environmental DNA for molecular ecological surveys has become widely utilized in ecological assessments of various water environments, from rivers to coastal and marine areas. However, it is important to consider the scenarios of potential contamination of environmental nucleic acids in interpreting the results. In this study, we analyzed the fish species present in the feed that may [...]
Unlocking the hidden dimensions of genomic diversity within species
Published: 2024-12-16
Subjects: Evolution, Genomics, Molecular Biology
The missing heritability problem, defined as the failure of genetic variants to explain variance in phenotypes, has been an unsolved issue in genetics for the past two decades. A potential solution to this problem stems from the idea that single nucleotide polymorphisms and copy-number variants, the most commonly studied forms of genomic diversity, do not represent the totality of the information [...]
Reassessing a Holocene extinction: multiple lines of evidence do not support the historical presence and recent extirpation of a protected anole on the island of Anguilla
Published: 2024-09-20
Subjects: Biodiversity, Genomics, Integrative Biology, Other Ecology and Evolutionary Biology, Zoology
Accurate assessment of historical species ranges is important for conservation science and management. Inaccurate historical species ranges can lead to incorrect assumptions about local extinctions, population trends, and potential sites for reintroductions. Yet, historical knowledge is often lacking for many species. Here, we examine the case of the bearded anole, Anolis pogus, which is long [...]
Demographic expansion and panmixia in a St. Martin endemic, Anolis pogus, coincides with the decline of a competitor
Published: 2024-09-19
Subjects: Biodiversity, Ecology and Evolutionary Biology, Evolution, Genomics, Population Biology, Zoology
Understanding patterns of differentiation at microgeographic scales can enhance our understanding of evolutionary dynamics and lead to the development of effective conservation strategies. In particular, high levels of landscape heterogeneity can strongly influence species abundances, genetic structure, and demographic trends. The bearded anole, Anolis pogus, is endemic to the topographically [...]
Not by selection alone: expanding the scope of gene-culture coevolution
Published: 2024-09-05
Subjects: Anthropology, Biological and Physical Anthropology, Ecology and Evolutionary Biology, Evolution, Genetics and Genomics, Genomics, Social and Behavioral Sciences
Gene-culture coevolution (GCC) - an ambitious synthesis of biological and social sciences - is often used to explain the evolution of key human traits. Despite the framework’s broad conceptual appeal however, empirical evidence is often perceived as limited to a few key examples like lactase persistence. We argue this apparent gap between theoretical appeal and empirical evidence stems [...]
The Genomics for Australian Plants (GAP) framework initiative – developing genomic resources for understanding the evolution and conservation of the Australian flora.
Published: 2024-07-08
Subjects: Biodiversity, Botany, Genomics
The generation and analysis of genome-scale data—genomics—is driving a rapid increase in plant biodiversity knowledge. However, the speed and complexity of technological advance in genomics presents challenges for its widescale use in evolutionary and conservation biology. Here, we introduce and describe a national-scale collaboration conceived to build genomic resources and capability for [...]
Evolution and maintenance of mtDNA gene content across eukaryotes
Published: 2024-04-22
Subjects: Evolution, Genetics, Genomics
Across eukaryotes, most genes required for mitochondrial function have been transferred to, or otherwise acquired by, the nucleus. Encoding genes in the nucleus has many advantages. So why do mitochondria retain any genes at all? Why does the set of mtDNA genes vary so much across different species? And how do species maintain functionality in the mtDNA genes they do retain? In this review we [...]
The nitroplast and its relatives support a universal model of features predicting gene retention in endosymbiont and organelle genomes
Published: 2024-04-22
Subjects: Evolution, Genomics
Endosymbiotic relationships have shaped eukaryotic life. As endosymbionts coevolve with their host, towards full integration as organelles, their genomes tend to shrink, with genes being completely lost or transferred to the host nucleus. Modern endosymbionts and organelles show diverse patterns of gene retention, and why some genes and not others are retained in these genomes is not fully [...]