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Preprints

Filtering by Subject: Life Sciences

Colossal Disinformation

Vincent Lynch

Published: 2026-05-20
Subjects: Biology, Ecology and Evolutionary Biology, Life Sciences

I have a position: de-extinction is almost impossible, however, deëxtinction, a word invented by the world’s first “de-extinction” company Colossal Laboratories & Bioscience’s can be (Max 2025); hereafter Colossal, which they define as the creation of genetically modified organisms (GMOs) that appear and act like extinct species, is possible. It’s branding, not science. I and other critics of [...]

The roles of density dependence, developmental asynchrony, and niche traversal costs in shaping the evolution of ontogenetic complexity

James G DuBose

Published: 2026-05-20
Subjects: Life Sciences

Most organisms undergo some degree of niche transition throughout their life cycles, which are typically accompanied by morphological, physiological, and/or behavioral changes. Ontogenetic complexity generally refers to the magnitude and abruptness of these changes. Evolutionary theory has described how various genetic properties facilitate and constrain the evolution of ontogenetic complexity, [...]

A Protocol for Standardizing Measurements and Enabling Global Harmonization of Herbarium Leaf Reflectance Spectra

Dawson M White, Flávia M Durgante, Matthew Austin, et al.

Published: 2026-05-20
Subjects: Biochemistry, Biophysics, and Structural Biology, Biodiversity, Bioinformatics, Botany, Ecology and Evolutionary Biology, Life Sciences, Other Ecology and Evolutionary Biology, Research Methods in Life Sciences

Reflectance spectroscopy offers a powerful approach to integrate high-throughput phenotypic data from herbarium specimens into the digital landscapes of ecology, evolution, and systematics. Because inconsistencies in instrumentation and measurement practices can increase noise and limit dataset compatibility, the International Herbarium Spectral Digitization Working Group (IHerbSpec) has [...]

Cheating and imperfect vaccines as drivers of bacterial evolution

Florian Lecorvaisier, Thomas Louis Philippe Martin

Published: 2026-05-20
Subjects: Life Sciences

Cheating is an ubiquitous evolutionary strategy, appearing everywhere throughout the tree of life. Among bacteria, cheating appears mostly through the consumption of public good without participation in their production. Some vaccines, because they specifically target these public goods, may alter the eco-evolutionary dynamics of cheating in bacterial populations of pathogenic species such as [...]

Oak masting remains stable despite climate warming

Michał Bogdziewicz, Jessie Foest, Jakub Szymkowiak, et al.

Published: 2026-05-20
Subjects: Ecology and Evolutionary Biology, Life Sciences

Climate warming alters tree reproduction, but long-term tests of how it affects the large, synchronised interannual fluctuations in seed production known as masting remain rare. Theory predicts that climate-driven declines in masting should be most likely when species combine high reproductive sensitivity to weather cues with rapid climatic change during the corresponding cue windows. We tested [...]

Parasites and forage as determinants of body condition and population size in an imperiled ungulate.

Benjamin Juan Padilla, Oscar Alejandro Aleuy, Petter Jacobsen, et al.

Published: 2026-05-20
Subjects: Life Sciences, Other Ecology and Evolutionary Biology, Parasitology, Population Biology

Rapid environmental changes are resulting in widespread changes in population size, health, and physiology of wildlife, especially at northern latitudes where the impacts of climate change are more pronounced. Barren-ground caribou (Rangifer tarandus groenlandicus) have declined across much of their range in recent decades, and while the ultimate causes are unknown, western science and local [...]

Designing for nature doesn't cost the Earth

Jacinta Ellesse Humphrey, Holly Louise Kirk, Victoria Cook, et al.

Published: 2026-05-19
Subjects: Biodiversity, Life Sciences, Terrestrial and Aquatic Ecology, Urban Studies and Planning

1. A key barrier to the development of nature positive cities is the unknown cost of implementing novel urban design elements. Strict budgets and government approval processes make it challenging for developers to trial new approaches, meaning most developments rarely deviate from ‘business-as-usual’ (BAU). Further research is urgently needed to overcome this barrier to innovation and help [...]

Towards comparability among state-and-transition models: A set of generalised templates linked to ecosystem condition

Suzanne M Prober, Megan Kate Good, Helen Murphy, et al.

Published: 2026-05-19
Subjects: Life Sciences

Growing commitments to environmental sustainability and nature conservation by industry, government and communities globally have led to a pressing need for consistent methods to characterise and quantify outcomes of land use and ecological restoration. State-and-transition models (STMs) are widely used to describe and communicate knowledge about ecosystem dynamics and are increasingly applied in [...]

Expanding the sentinel approach through multimodal integration: resolving underlying ecological processes with eDNA and computer vision

Yuval Cohen, Jordan Patrick Cuff, Liora Shaltiel-Harpaz

Published: 2026-05-19
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Life Sciences, Other Ecology and Evolutionary Biology, Research Methods in Life Sciences, Terrestrial and Aquatic Ecology

Sentinel approaches provide a semi-controlled method for quantifying in-field ecological interactions and processes while reducing bias and labour. They are, however, limited by difficulties ascribing taxonomic identities, behavioural context and temporal resolution to interacting agents. The integration of additional sources of data, including the analysis of DNA left behind on sentinel objects [...]

Feeding Ecology and Conservation of the Mediterranean Monk Seal, (Monachus monachus)

Ines Moreira Santos, Rebecca LST Netels, Ashlie J. McIvor, et al.

Published: 2026-05-19
Subjects: Life Sciences

The Mediterranean monk seal (Monachus monachus) is one of the world’s most endangered pinnipeds, persisting as a small number of fragmented populations exposed to continuing anthropogenic pressure. Understanding its feeding ecology is therefore important not only for clarifying its trophic role and habitat use, but also for informing conservation actions related to fisheries overlap, prey [...]

Reframing the habitat fragmentation debate around the inferential targets predicted by ecological theory

Juan Andrés Martínez Lanfranco, Erin M. Bayne

Published: 2026-05-14
Subjects: Life Sciences

The habitat fragmentation debate has persisted for more than three decades because dominant empirical practice has often estimated a narrower quantity than the competing ecological theories jointly require. These frameworks differ not simply in whether fragmentation matters, but in how their predicted effects change along the habitat-amount gradient. The habitat amount hypothesis (HAH) predicts [...]

The Global Biodiversity Framework supports global assessment of One Health actions

Francis Banville, Claire Burnel, Colin J Carlson, et al.

Published: 2026-05-14
Subjects: Biodiversity, Environmental Health and Protection, Environmental Monitoring, Life Sciences, Public Health

The One Health approach promotes collaboration across disciplines to enhance the health of humans, animals, plants, and the environment. Recently developed by the Quadripartite organizations, the One Health Joint Plan of Action (2022-2026) supports countries in adopting the One Health approach through six action tracks. The tracks address multiple aspects of biodiversity, containing guiding [...]

Making use of oak genomes

Andrew L Hipp, Antoine Kremer

Published: 2026-05-13
Subjects: Biodiversity, Botany, Genomics, Life Sciences

This review summarizes the contributions of genomics to our understanding of oak evolution and management, both past and ongoing. Far from being exhaustive given the large number of publications following the publication of the genomes, this review emphasizes work conducted in the decade following publication of the first two complete oak genome assemblies, and major findings and achievements [...]

HARBOUR PORPOISE RESPONSES TO PILE DRIVING ARE BETTER PREDICTED BY DISTANCE TO SOURCE THAN BY ENERGY-BASED RECEIVED SOUND LEVELS

Paul M Thompson, Aude Benhemma-Le Gall, Barbara Cheney, et al.

Published: 2026-05-13
Subjects: Life Sciences

1. Regulatory assessments for offshore construction are required to avoid impacts on protected marine mammals through noise-related injury or disturbance. Criteria for injury risk are widely accepted, but the extent to which behavioural responses are related to noise levels or co-varying contextual factors such as proximity remains uncertain. 2. This study used arrays of echolocation detectors [...]

Livestock subsidise tiger diets in a central Indian corridor: implications for human-wildlife conflict management and conservation planning

Suvankar Biswas, Sparsh Dubey, Pranav Chanchani, et al.

Published: 2026-05-12
Subjects: Life Sciences

Large carnivores, like tigers, maintain a balance within their respective ecosystem and play a critical role. However, due to their life history needs and extensive overlap with humans, they face significant threats across their distribution. These threats become severe when they inhabit non-protected areas like corridors and kill livestock. In this study, the food habits of tiger were assessed [...]

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