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Preprints

Filtering by Subject: Life Sciences

Beyond Observed Diversity: A Completeness-Based Invasion Theory

Yanjie Liu

Published: 2026-02-26
Subjects: Ecology and Evolutionary Biology, Life Sciences, Other Ecology and Evolutionary Biology, Population Biology, Terrestrial and Aquatic Ecology

Charles Elton proposed that species-rich communities resist invasion better, but support is mainly from local studies, possibly because studies use observed richness alone, ignoring the dark diversity. I propose Completeness-Based Invasion Theory, linking invasibility inversely to community completeness, an index linking observed and dark diversity, enabling unified insights across scales.

Tapping into symbiosis to advance human microbiome research

Gina R Lewin, Lily Khadempour

Published: 2026-02-25
Subjects: Bacteriology, Ecology and Evolutionary Biology, Environmental Microbiology and Microbial Ecology Life Sciences, Life Sciences, Other Microbiology, Pathogenic Microbiology

In human microbiome research, the term commensal is often used to describe organisms that benefit their hosts. In ecology, where the term originates, a commensal organism has no impact on its host, whereas a mutualist organism benefits its host. While others have recognized this discrepancy in terminology use, old habits are hard to break, and the human microbiome community has continued in this [...]

A new conceptual framework for host-microbe symbiosis

Lily Khadempour

Published: 2026-02-25
Subjects: Ecology and Evolutionary Biology, Life Sciences

Host-microbe relationships are studied across biological disciplines, with different but related discipline-specific conceptual frameworks arising from each of them. Without a unified framework that can be applied across all symbioses, we cannot do the interdisciplinary work necessary to understand the underlying rules that govern symbiosis. Here I present a new conceptual framework for [...]

Stronger Evidence for Trait–Environment Association by Pre-processing of Abundance Tables

Cajo ter Braak

Published: 2026-02-25
Subjects: Life Sciences

Understanding trait–environment relationships is central to predicting community responses to environmental change, yet statistical evidence for such relationships is often weak in observational datasets. Here, I introduce an N2-processing method for abundance tables, grounded in the observation that the precision of community weighted means (CWMs) and species niche centroids (SNCs) is [...]

High-Resolution Coastal Blue Carbon Site Intelligence: A Multi-Attribute Geospatial Pipeline for National-Scale Mangrove Assessment

Jayson Gutierrez

Published: 2026-02-25
Subjects: Biodiversity, Bioinformatics, Life Sciences, Systems Biology

The voluntary blue carbon market is severely bottlenecked by outdated methodologies that apply broad, coast-level carbon averages across low-resolution spatial units, systematically failing to account for micro-site ecological realities and critical socio-political constraints. To resolve this structural deficit, this paper introduces the High-Resolution Geographically-Explicit Blue Carbon [...]

The Global Lakes Explorer: A basin-to-global scale data visualisation application for the assessment of nutrient emissions to lakes

Elise Gallois, Philip Taylor, Will J Brownlie, et al.

Published: 2026-02-24
Subjects: Computer Sciences, Earth Sciences, Environmental Sciences, Life Sciences, Physical Sciences and Mathematics

Phosphorus and nitrogen are critical plant nutrients, essential for fertiliser production and global food security. However, poor management across the anthropogenic nutrient cycles leads to losses associated with pollution of water bodies, driving eutrophication, biodiversity loss, and methane emissions. Addressing these interconnected challenges requires a sustainable, integral, and where [...]

BABAPPAΩ: Diagnosing the Identifiability of Episodic Selection under Branch–Site Evolution Using Likelihood-Free Neural Inference

Krishnendu Sinha

Published: 2026-02-24
Subjects: Bioinformatics, Life Sciences

Episodic positive selection acting on specific evolutionary lineages is a longstanding yet intrinsically difficult target of molecular inference. Classical branch–site methods formulate this problem as hypothesis testing under explicit codon substitution models, implicitly assuming that episodic selection is statistically identifiable from finite alignments. Under biologically realistic [...]

A Niche in the Machine: The Promise of AI Foundation Models for Species Distribution Modeling

Russell Dinnage, Dan L. Warren

Published: 2026-02-24
Subjects: Ecology and Evolutionary Biology, Life Sciences

Species distribution models (SDM) are fundamental tools for conservation, yet methodological progress has stalled. Despite two decades of refinement, traditional approaches – MaxEnt, boosted regression trees, random forests – have approached a performance ceiling, and deep learning has failed to break through on species distribution data. TabPFN, a foundation model that learns to perform Bayesian [...]

Global Patterns Predict Local Biodiversity Shifts in a Climate Change Hotspot

Jake Lawlor, Amelia Hesketh, Julien Beaulieu, et al.

Published: 2026-02-23
Subjects: Life Sciences

Climate change is redistributing life on Earth, and global-scale biogeographical patterns can inform expectations for local ecological responses. As thermal envelopes shift towards higher absolute latitudes and deeper depths in the ocean, fixed locations are experiencing changes in their niche space, driving changes in abundance, occurrence, and community composition. Here, we examine intertidal [...]

A new effect size for meta-analysis of magnitude: lnM

Shinichi Nakagawa, Ayumi Mizuno, Coralie Williams, et al.

Published: 2026-02-23
Subjects: Ecology and Evolutionary Biology, Life Sciences, Statistics and Probability

Meta-analyses in ecology and evolution often consider the magnitude of differences between groups rather than their direction. Yet, a common practice is to coerce signed effects (e.g., d and response ratio) into magnitudes by taking absolute values. This transformation induces strong upward bias and non-normal (Gaussian) sampling distributions, violating the assumptions of standard meta-analytic [...]

A Merlin Falco columbarius at the southern fringe of its Asian wintering range in Madhya Pradesh, India

Mandar Tijare

Published: 2026-02-23
Subjects: Life Sciences

The Merlin Falco columbarius is a small falcon considered an uncommon winter visitor to northwestern India, with records from the central part of the country being extremely scarce and the species often regarded as accidental in peninsular India. Here, I report a rare, well-documented observation of a Merlin from Bhopal, Madhya Pradesh, in central India. The bird was observed and photographed at [...]

Kin recognition in non-native plants: a general hypothesis of invasiveness

Rameez Ahmad, Yanjie Liu

Published: 2026-02-20
Subjects: Life Sciences

Understanding how non-native plants successfully invade new environments is a fundamental question in invasion ecology. Here, we propose a novel hypothesis of kin recognition - the ability of plants to differentiate between closely related and distantly related neighbors - as a mechanistic explanation for invasion success. To evaluate the idea, we reviewed existing evidence for kin recognition in [...]

Composite virulence: useful metric or conceptual trap?

Luis M. Silva, Tiago G. Zeferino

Published: 2026-02-20
Subjects: Animal Diseases, Animal Experimentation and Research, Animal Sciences, Biodiversity, Biology, Ecology and Evolutionary Biology, Evolution, Immunity, Immunology and Infectious Disease, Immunology of Infectious Disease, Immunopathology, Life Sciences, Medical Microbiology, Microbiology, Other Ecology and Evolutionary Biology, Other Immunology and Infectious Disease, Parasitic Diseases, Parasitology, Pathogenic Microbiology, Plant Pathology, Research Methods in Life Sciences, Zoology

Virulence, the harm an infection causes to its host, is a cornerstone concept in ecology and evolution, yet it remains difficult to quantify because infection impact is multidimensional, dynamic, and context-dependent. Infections can reduce host performance through multiple, partially redundant routes (including mortality, fecundity loss, behavioural impairment, and physiological disruption), [...]

Anergiobiosis: a testable framework for microbial life under extreme power limitation

Paul Carini, Roland Hatzenpichler, Jennifer F Biddle

Published: 2026-02-20
Subjects: Biology, Life Sciences, Marine Biology, Microbiology

"Aeonophily" was recently suggested as a new category of extremophily for ultra-slow-growing subsurface microorganisms. This terminology conflates a physiological state with potential extremophilic specialization. We propose "anergiobiosis" to describe life without sufficient power to sustain cell division, separating this state from questions about specialization. Analogous to temperature [...]

A Framework for Questionable Research Practices in Ecological Modelling

Elliot Gould, Hannah S. Fraser, Bonnie Claire Wintle, et al.

Published: 2026-02-20
Subjects: Applied Statistics, Ecology and Evolutionary Biology, Life Sciences, Natural Resources and Conservation, Water Resource Management

1. Questionable research practices (QRPs) bias the published literature towards apparently strong and conclusive results, resulting in low rates of replicability. Recent metaresearch reveals that ecology is not immune to the ‘reproducibility crisis’ seen in other disciplines, due to similar rates of QRPs and a lack of transparency in published research. However, metaresearch to date focuses on [...]

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