Preprints
Filtering by Subject: Life Sciences
Evaluating population resilience to anticipated stressors using integrated population modeling: a case study of Peregrine Falcons
Published: 2026-03-15
Subjects: Biodiversity, Biology, Ecology and Evolutionary Biology, Life Sciences, Ornithology, Population Biology
Reliable estimates of demographic parameters are fundamental to understanding population dynamics and guiding conservation efforts. Integrated population models (IPMs) provide a powerful framework for jointly analyzing diverse data sources to estimate demographic rates and population trajectories, evaluate resilience to environmental stressors, and project population dynamics info the future. We [...]
A widespread gap in U.S. Endangered Species Act implementation: Risk of genetic erosion within populations
Published: 2026-03-15
Subjects: Biodiversity, Life Sciences
Despite its importance to fitness and population viability, genetic diversity is rarely incorporated into biodiversity assessments. The recent adoption of indicators of intraspecific genetic diversity by the Convention on Biological Diversity has highlighted the importance of evaluating genetic diversity in wild species. Genetic indicators are useful even in the absence of genetic data because [...]
Assessing the dual utility of nuptial patterning in the rainbow darter (Etheostoma caeruleum)
Published: 2026-03-15
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Life Sciences
In this study, we assessed the possible dual utility of nuptial color patterns in male rainbow darters (Etheostoma caeruleum) in the context of male-male territorial interactions and female mate choice. Using a cohort of wild-caught male E. caeruleum, we conducted simulated territorial contests between pairs of males in the lab. We scored the “winner” and the “loser” of each contest based on [...]
Impacted Bones: Can extant primates help identify tool-use in early hominins?
Published: 2026-03-15
Subjects: Life Sciences
Despite recent advances pushing back the earliest record of tool use, how and when it first emerged in the hominin lineage, and the extent to which it featured amongst the numerous co-existing hominin species, remain critical questions in palaeoanthropology. In addition to analysing extinct hominin fossils and lithic toolkits, tool-use in extant primates should be studied to help answer this [...]
The use of multi-response models to improve inferences about natural selection
Published: 2026-03-14
Subjects: Life Sciences
Natural selection, the relationship between trait and fitness, is a key determinant of evolutionary change and population adaptation. Therefore, accurate estimation of natural selection is important. In 1983, Lande and Arnold proposed a simple regression-based approach which allows the measurement of selection on a range of traits whilst accounting for confounding variables. However, issues [...]
Drivers of roe deer use in fragmented forest landscapes; implications for management in the context of policy driven forest expansion
Published: 2026-03-13
Subjects: Animal Sciences, Behavior and Ethology, Ecology and Evolutionary Biology, Forest Management, Forest Sciences, Life Sciences, Zoology
Forest expansion is a major current land use change across Europe. How this will affect the forest use of the most common European wild ungulate species, roe deer (Capreolus capreolus), and associated forest ecosystem services and disservices is poorly understood. Using a forest-agricultural mosaic landscape in northeast Scotland, we selected forests across a size and connectivity gradient as a [...]
Photographs and observations suggest Superb Fairy-wren Malurus cyaneus x Variegated Fairy-wren Malurus lamberti hybridisation
Published: 2026-03-12
Subjects: Life Sciences
We observed and photographed an unusual looking male Fairy-wren at Lake Eden, just north of Brisbane, Queensland. The plumage of this individual displays a blend of Superb Fairy-wren Malurus cyaneus and Variegated Fairy-wren Malurus lamberti characteristics, supporting the notion of a novel hybridisation event between these two species. We have observed this likely hybrid on multiple occasions in [...]
An update on the Black-naped Pheasant-pigeon and search for Long-billed Myzomela on Fergusson Island, Papua New Guinea
Published: 2026-03-12
Subjects: Life Sciences
Searching for lost species– those without conclusive documentation for a decade or longer– has emerged as a core goal of conservationists during the current extinction crisis. While documenting these species is an important first step, sustained efforts to study and conserve these often data-deficient and rare taxa are necessary to increase prospects for long-term survival. Here we report on an [...]
The lens of the Sonic Holobiont. A perspective on acoustic influence on microbial communities and its application as an additional layer to the holobiont concept.
Published: 2026-03-11
Subjects: Arts and Humanities, Biochemistry, Biophysics, and Structural Biology, Biodiversity, Ecology and Evolutionary Biology, Life Sciences, Microbiology, Other Arts and Humanities, Other Physical Sciences and Mathematics, Physical Sciences and Mathematics
When studying micro and macro biomes in the quest for a more general understanding, we can hardly escape from a holistic perspective. At first, symbiosis was demonstrated to be a ubiquitous phenomenon in living cells, shaping evolutionary patterns across species at very different scales. The “holobiont” concept gains a central role in modern biology. The observation of the complex inter- and [...]
Global latitudinal and bathymetric gradients in body size among cartilaginous fishes (Gnathostomata: Chondrichthyes)
Published: 2026-03-11
Subjects: Ecology and Evolutionary Biology, Evolution, Life Sciences, Other Ecology and Evolutionary Biology, Terrestrial and Aquatic Ecology
Understanding the macroecological rules governing body size variation across environmental gradients has long been a central focus of biology for centuries. Bergmann’s rule – the tendency for animals to reach larger body sizes in colder environments – has been studied in endotherms but with mixed support. However, phylogenetically informed tests of this rule in ectotherms remain scarce, and there [...]
Why your causal diagram should probably include sampling and measurement processes
Published: 2026-03-11
Subjects: Life Sciences
Insect scientists are starting to use causal diagrams to display assumptions about causal relationships between variables that exist before any data have been collected. The perception appears to be that these assumptions are sufficient to determine whether observed associations between variables can be interpreted causally. But an observed association implies an observation process (sampling and [...]
Reconstructing the Holy Loch Food Web: A simple system for interpreting large numbers of Barcode of Life Database (BOLD) Biodiversity Index Numbers (BINs) for use in taxonomy, natural history and conservation, using Chironomoidea (Diptera) as a model taxon.
Published: 2026-03-11
Subjects: Life Sciences
Mass, low-cost-, non-destructive DNA sequencing/barcoding via UK BIOSCAN, based at the Wellcome Sanger Institute, is now creating hundreds of thousands of invertebrate Cytochrome c Oxidase sub unit 1 (COI) barcodes from specimens collected across the United Kingdom (UK). Holy Loch Nature Reserve (HLNR), in Argyll, Scotland, joined the project in 2024, and in the same year, our first 6350 DNA [...]
Evaluating the efficacy of window treatments to reduce bird–window collisions
Published: 2026-03-11
Subjects: Life Sciences
Collisions with glass are a leading anthropogenic driver of avian mortality. Window treatments can be highly effective at reducing collisions, however, treating windows remains uncommon and many treatments are applied that do not follow evidence-based standards or guidelines. To evaluate the efficacy of window treatments that meet versus do not meet guidelines, and the drivers influencing [...]
Separating good from bad – a methodological assessment of the critical temperature that separates stressful and permissive temperatures in ectotherms
Published: 2026-03-11
Subjects: Ecology and Evolutionary Biology, Life Sciences
Context and aim: Estimating the thermal limits of ectothermic organisms is critical for predicting their responses to climate change. A key physiological threshold in this context is the critical temperature (Tc), which separates the permissive temperature range, where organisms maintain homeostasis and complete their life cycle, from the stressful range, where thermal stress causes physiological [...]
Conserving Coherence Under Constraint
Published: 2026-03-10
Subjects: Life Sciences
Organisms often respond to energy constraints, time pressure, or imminent threat by limiting behavioral options, lowering metabolic demands, and increasing their level of coordinated action. Although these responses can be framed as impairment, we argue they can be adaptive responses that occur as the costs of coordinating complexity exceed an organism's capabilities. As such, selection favors [...]