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Preprints

Filtering by Subject: Life Sciences

Choosing the Response Matrix: Generalised Linear Latent Variable Models for Multivariate Ecology and Evolution

Shinichi Nakagawa, Ayumi Mizuno, Russell Dinnage, et al.

Published: 2026-06-24
Subjects: Ecology and Evolutionary Biology, Life Sciences

Multivariate responses are central to ecology and evolutionary biology, but their covariance is often difficult to model and interpret. Generalised linear latent variable models (GLLVMs) provide a parsimonious way to represent covariance among many responses using a smaller number of latent variables. They are widely used for Site X Species data in joint species distribution modelling and [...]

Capsicum pubescens Ruiz & Pav.: evolutionary history, genetic resources and future opportunities for an overlooked Andean chile

Nahuel Ezequiel Palombo, Marisel Analía Scaldaferro, Carolina Carrizo García

Published: 2026-06-24
Subjects: Life Sciences

Growing concerns over food security, agrobiodiversity loss, and climate change are driving renewed interest in neglected and underutilized crops with high agronomic, nutritional, and adaptive potential. Capsicum pubescens Ruiz & Pav. is one of the five domesticated chile pepper species and a distinctive crop of Andean agriculture. Adapted to cool mountain environments and characterized by its [...]

Droplet-induced surface aeration, not acoustic sensing, most parsimoniously explains accelerated germination of submerged rice seeds

Ariel Novoplansky

Published: 2026-06-24
Subjects: Life Sciences

When water drops strike the surface above submerged rice seeds, the seeds germinate faster, an effect that scales with drop height and falls off sharply with distance. Makris and Navarro1 attributed this to acoustic stimulation of statoliths, specialised gravity-sensing organelles, suggesting seeds can effectively sense the sound of raindrops. Here I argue that a simpler, well-established [...]

Structural and functional skin microbiota on cane toads across 16,000 km of invaded range

Chava L Weitzman, Kimberley A Day, Gregory Brown, et al.

Published: 2026-06-23
Subjects: Life Sciences

Host-associated microbial communities are shaped by environmental availability, host filtering, microbial interactions, and prior pathogen exposure, with connected habitats promoting greater adaptive microbiome potential. Across the invasive range of cane toads, containing expansive disconnects between populations, we found strong spatial variation in skin bacterial communities, including among [...]

The Fish Fauna of Tubbataha Reefs is highly Biodiverse and distinctively Oceanic

Gerlie T. Gedoria, Klaus Stiefel, Jeffrey T. Williams, et al.

Published: 2026-06-23
Subjects: Biodiversity, Life Sciences, Marine Biology, Zoology

We surveyed the fish fauna of Tubbataha Reefs, a remote and well-protected coral reef system in the Philippines. Tubbataha is located in the Coral Triangle, the region with the highest marine biodiversity in the world, and is a no-take marine protected area. We found a total of 534 species, with the Labridae (65 species), Pomacentridae (60 species), Gobiidae (60 species), Chaetodonidae (33 [...]

Visual-chemotactic saltatory search in Octopus hummelincki (Mollusca, Cephalopoda): a case study in the South Atlantic

Michaella Pereira Andrade, Flavio Ayrosa, Charles MD Santos, et al.

Published: 2026-06-23
Subjects: Behavior and Ethology, Ecology and Evolutionary Biology, Life Sciences

We report for the first time evidence of foraging by Octopus hummelincki and analyze it using saltatory search theory, which posits alternating phases of locomotion and stationary search. Our data showed that substrate complexity dictates behavioral transitions: locomotion predominated in sand, whereas solid substrates elicited tactile exploration. The move-to-search scaling ratio (0.63) aligns [...]

Body condition, but not reproductive success, is associated with sociality in a colonial seabird.

Antoine Morel, Pierre-Paul Bitton

Published: 2026-06-19
Subjects: Biodiversity, Life Sciences, Marine Biology, Ornithology, Zoology

Body condition, breeding habitat quality and access to socially acquired information are generally associated with higher fitness in social animals. In colonial species that breed in dense aggregations, such as seabirds, the combined effects of these factors on reproductive success have rarely been tested together. In this study, we investigated the relationship between fledging success, body [...]

Governing for Learning: Institutional Foundations of Effective Adaptive Management

Fred Allen Johnson, William Pine

Published: 2026-06-19
Subjects: Life Sciences

Adaptive management (AM) remains one of the most promising frameworks for managing conservation challenges under uncertainty, yet its potential is rarely realized in practice. Conservation agencies routinely make repeated decisions under uncertainty, but those decisions are often not structured in ways that allow learning to accumulate and improve future choices. This Perspectives article argues [...]

Algorithmically-controlled ecosystems and biodiversity

Matthias C. Rillig, David W Armitage, Jun Tani, et al.

Published: 2026-06-18
Subjects: Life Sciences

Algorithmically-controlled ecosystems are ecosystems in which at least one key process rate or property (e.g. biodiversity) is under control by algorithms, or if ecosystems contain robots/ machines. Algorithmic influence on ecosystems will be matter of degree, and we highlight the risks and opportunities of such algorithm-influenced ecosystems, as well as the need to have discussions about [...]

A mathematical foundation of modelling thermal injury and repair dynamics in ectotherms

Andreas Havbro Faber, Peter Borgen, Bodil Kirstine Ehlers, et al.

Published: 2026-06-18
Subjects: Animal Experimentation and Research, Ecology and Evolutionary Biology, Environmental Indicators and Impact Assessment, Environmental Sciences, Evolution, Life Sciences, Physiology, Plant Sciences, Population Biology, Research Methods in Life Sciences, Statistical Models, Statistics and Probability, Survival Analysis

As global temperatures rise and extreme heat events impair ectotherm performance and survival, it is becoming increasingly important to predict how organisms accumulate and repair thermal injury under realistic benign and stressful temperatures. The thermal death time (TDT) model quantifies how heat events translate into thermal injury, but under natural temperature fluctuations the TDT model is [...]

What Artificial Intelligence Cannot Replace in Ecology?

Juline Rodrigues da Conceição

Published: 2026-06-18
Subjects: Life Sciences

Artificial intelligence is becoming increasingly integrated into ecological research, from literature synthesis and hypothesis generation to statistical modelling and data analysis. As AI systems become more autonomous, discussions about ecology's future are often framed as a competition between human and machine intelligence. Yet this perspective overlooks a more fundamental question: what [...]

Mind the Gap: A critical 40-fold workforce shortfall for protecting 30% of the ocean

Christine A Ward-Paige, Alexandre Courtiol, Michael R Appleton, et al.

Published: 2026-06-18
Subjects: Life Sciences, Marine Biology, Other Social and Behavioral Sciences, Public Economics

Despite being tasked with protecting ocean biodiversity, the marine protected area (MPA) workforce remains poorly described. Using a global site-level questionnaire, we assessed staffing, workforce adequacy, technology use, and personnel requirements for effectively and equitably conserving 30% of the ocean by 2030 (30 x 30). Responses represented 52.8% of global MPA area across 63 countries and [...]

The first systematic map of evidence syntheses on the use of artificial intelligence in ecology

Sergio Poo Hernandez, Eduardo S. A. Santos, Santiao Ortega, et al.

Published: 2026-06-17
Subjects: Ecology and Evolutionary Biology, Life Sciences

Artificial intelligence (AI) is increasingly used in ecology to automate data-intensive tasks, from species identification and environmental monitoring to ecological prediction. As primary studies have proliferated, evidence syntheses reviewing AI applications have also increased, but their thematic coverage, methodological emphasis, and reporting transparency remain unclear. We conducted a [...]

Saved by the Symbiont: Environmental Stress Intensity and Endosymbiont-Mediated Stress Response Determine Evolved Host Complexity

Kiara M. Johnson, Camila Mendoza, Daya Tucker, et al.

Published: 2026-06-17
Subjects: Evolution, Life Sciences

Understanding how stress responses affect the trajectory of host–symbiont coevolution is central to predicting and managing species outcomes in the face of disturbances to ecosystems. Critically, it remains an open question how exactly we expect stressors to influence the coevolutionary dynamics of symbioses (on either end of the parasitism–mutualism continuum). In this work, we use in silico [...]

prepR4pcm: An R Package for Preparing Data and Trees for Phylo- genetic Comparative Methods

Shinichi Nakagawa, Santiao Ortega, Ayumi Mizuno, et al.

Published: 2026-06-17
Subjects: Ecology and Evolutionary Biology, Life Sciences

1. Phylogenetic comparative methods require species names in a trait dataset to match tip labels in a phylogenetic tree. Yet this apparently simple prerequisite is often one of the most fragile steps in a comparative workflow. Names may differ because of, for example, formatting, taxonomic revisions, synonyms, or spelling errors. If these differences are resolved informally, species can be lost [...]

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