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Preprints

Filtering by Subject: Life Sciences

Tree species richness effects on pre-dispersal seed predation are mediated by tree fruit type

FEILONG JI, Noga Abecassis-Monteyne, Xiaojuan Liu, et al.

Published: 2026-06-12
Subjects: Biodiversity, Ecology and Evolutionary Biology, Forest Sciences, Life Sciences

Forest BEF experiments are only now reaching a stage at which natural tree regeneration can be studied, offering new opportunities to understand how biodiversity shapes trophic interactions during early demographic filtering. Here, we quantified seed productivity and insect-mediated pre-dispersal seed predation on 12 tree species across a tree species richness gradient from 1 to 16 in the [...]

An integrated framework for unifying our understanding of nonconsumptive predation risk effects

Andrew Thomas Davidson, Tal Avgar, Daniel MacNulty, et al.

Published: 2026-06-12
Subjects: Behavior and Ethology, Biology, Ecology and Evolutionary Biology, Evolution, Integrative Biology, Life Sciences, Other Ecology and Evolutionary Biology, Population Biology, Terrestrial and Aquatic Ecology

Predation risk can induce risk-induced trait responses (RITRs) – changes in prey defensive traits including behavior, morphology, life history, and physiology – thought to have profound effects on prey fitness and population dynamics (termed ‘nonconsumptive effects’). Yet, predicting the magnitude of RITRs and their fitness consequences remains difficult because outcomes depend heavily on [...]

Evolutionary and operational trade-offs in assisted gene flow for climate-adaptive forestry

Camilla Stefanini, Jannis Bolzern, Katalin Csilléry

Published: 2026-06-11
Subjects: Ecology and Evolutionary Biology, Life Sciences

Assisted gene flow (AGF) is an adaptive forest management strategy to increase forests' resilience to climate change, yet little is known about how management decisions interact with the strength of natural selection and introgression dynamics that co-determine relative stand productivity. We used individual-based, spatially explicit simulations to investigate how spatial configuration (ranging [...]

The crabeater seal reference genome reveals hallmarks of persistently large effective population size and sustained population expansion in the World’s most abundant pinniped

Beril Yildiz, Thomas Gelatt, Luis A. Hückstädt, et al.

Published: 2026-06-11
Subjects: Life Sciences

Population genetic theory predicts that a species’ demographic history shapes patterns of genome-wide variation. However, conservation genomic studies have disproportionately focused on small or declining species, where low genetic diversity and inbreeding are major concerns, while highly abundant species have attracted comparatively less attention. Here, we investigate the crabeater seal [...]

Predator Experience Shapes Behaviour: Comparing Stone Wētā (Hemideina maori) Populations With and Without Weka (Gallirallus australis hectori)

Sheri Johnson, Luke Thompson, Hamish Doogan, et al.

Published: 2026-06-10
Subjects: Biology, Ecology and Evolutionary Biology, Entomology, Life Sciences

Antipredator behaviour reflects both evolutionary history and individual experience, yet how populations respond to changes in predator exposure remains poorly understood, particularly for large invertebrates. We examined antipredator behaviour in two populations of stone wētā (Hemideina maori Pictet & Saussure, 1891) inhabiting weka-free Mou Tapu and nearby Mou Waho, where weka (Gallirallus [...]

Mapping Mechanistic Modeling of Highly Pathogenic Avian Influenza Across Migratory Flyways: A Systematic Review

Zubair Ahmad, Hao Wang, Simon Plakolb, et al.

Published: 2026-06-09
Subjects: Applied Mathematics, Diseases, Epidemiology, Life Sciences, Population Biology, Virus Diseases

Highly pathogenic avian influenza (HPAI) viruses, particularly H5N1 clade 2.3.4.4b, continue to spread globally via wild migratory birds along defined flyway corridors. Mechanistic models are essential tools for understanding HPAI transmission dynamics in flyway systems. Yet the geographic distribution of such modeling efforts across migratory flyways remains unknown. To inform the global [...]

Soma as transmission control in multicellular evolution: a population-genetic framework for germline restriction and cellular altruism

Juan Rivas-Santisteban, Juan F. Poyatos

Published: 2026-06-09
Subjects: Genetics, Integrative Biology, Life Sciences, Other Ecology and Evolutionary Biology

A central question in the evolution of multicellularity is why lineages repeatedly transition from germ-dominated unicellular states to organizations with extensive somatic investment. If somatic cells are largely excluded from future generations, why are they produced at all, and why do soma-free multicellular alternatives appear limited in stability, persistence, or attainable complexity? Here, [...]

Intertidal Exposure Modulates Time-Integrated Heat Tolerance of the Eastern Oyster Crassostrea virginica

Andrew R Villeneuve, Brittany M Jellison, Easton R White

Published: 2026-06-08
Subjects: Ecology and Evolutionary Biology, Integrative Biology, Life Sciences, Marine Biology, Other Ecology and Evolutionary Biology

The rise of unprecedented heatwaves globally has caused an increase in mass mortality events, motivating the need for accurate predictions of population declines. Predicting organismal function under fluctuating thermal regimes is a central challenge in thermal biology, particularly in intertidal systems where organisms experience rapid shifts between submerged and aerial exposure. Here, we [...]

Comparative urban behaviour of two sympatric columbids: Columba palumbus and Columba livia

Iñigo Navidad Larraona, Zaida Ortega Diago

Published: 2026-06-05
Subjects: Life Sciences

Urban environments favour species tolerant of human disturbance. We compared time budgets and anti-predator behaviour of the common wood pigeon (Columba palumbus) and rock pigeon (Columba livia) in five urban parks in León (Spain) using video recordings and predator approach tests. The common wood pigeon devoted most time to foraging (81.1%) with moderate vigilance (9.9%), while the rock pigeon [...]

Trophic interactions of ants are robust to tree species loss

Joshua Emil Spitz, Annika Leah Langlotz, Julian Lunow, et al.

Published: 2026-06-05
Subjects: Entomology, Life Sciences, Terrestrial and Aquatic Ecology

1. How changes in habitat conditions influence insect diversity has been intensively studied. However, whether trophic interactions of insects are also influenced by such changes is largely unknown. Higher habitat heterogeneity is often hypothesized to promote niche partitioning and complementarity in resource use among interacting species, yet evidence from animal interaction networks is sparse. [...]

Problems of geometry, sampling, and scale in gridded biodiversity data, and proposed solutions

Petr Keil, Friederike Wölke, Gabriel Ortega-Solis, et al.

Published: 2026-06-03
Subjects: Ecology and Evolutionary Biology, Life Sciences

Grids, and gridded biodiversity data such as regional or country-level atlases, play a prominent role in ecology, particularly in the study of spatial patterns of species occupancy, geographic ranges, biodiversity, and their drivers and temporal dynamics. However, managing, exploring, and analyzing data in grids comes with problems. Here, we review the problems with gridded data, and the existing [...]

Predicting substrate size at a watershed scale to inform conservation planning for a declining salmonid species

Kyleisha J. Foote, Shawn J. Leroux, Ava J. Hart, et al.

Published: 2026-06-02
Subjects: Biology, Earth Sciences, Ecology and Evolutionary Biology, Environmental Sciences, Hydrology, Life Sciences, Natural Resources and Conservation, Physical Sciences and Mathematics

Good quality spawning habitat is critical for fish embryo development, survival, and overall population productivity. Appropriate riverbed substrate size is particularly important for riverine-spawning salmonids but the availability of suitable substrate may vary across a watershed. Predicting substrate size at watershed extents may therefore be critical to inform management and conservation of [...]

From social experience to social behaviour: hormonal and behavioural phenotypes during adolescence in male guinea pigs

Melanie Gleske, S. Helene Richter, Sylvia Kaiser

Published: 2026-06-02
Subjects: Animal Sciences, Biology, Life Sciences, Social and Behavioral Sciences

Adolescence is the transition from juvenility to adulthood and is characterized by prominent endocrine, neural and behavioural alterations. Thus, adolescence represents a sensitive phase during which social experiences can shape endocrine and behavioural phenotypes. Although the influence of the social environment during adolescence has been widely investigated, most studies assessed such effects [...]

Concise guidelines and complementary checklists for improving research reliability and reproducibility

Jason Pither, Mathew Vis-Dunbar

Published: 2026-06-02
Subjects: Life Sciences

Checklists to address reproducibility shortfalls have proliferated but tend to be discipline- or research stage-specific and lack context or learning opportunities. We offer an alternative: one page, every stage, with a reason for each recommendation.

Evaluating the potential of molecular dietary analysis of predators for the detection of emerging plant pests

Kyle A Miller, Molly Davidson, Chris Hirst, et al.

Published: 2026-06-01
Subjects: Agriculture, Behavior and Ethology, Biodiversity, Biosecurity, Ecology and Evolutionary Biology, Entomology, Forest Biology, Forest Management, Forest Sciences, Genetics, Laboratory and Basic Science Research Life Sciences, Life Sciences, Research Methods in Life Sciences

Monitoring plant pests is crucial for maximising yields across agricultural and forest production systems, but also for the mitigation of invasive species spread. Traditional monitoring methods, such as mass trapping and direct observation, scale poorly and introduce latency between collection, detection and response. Since many plant pests are frequently consumed by predators, molecular dietary [...]

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