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Preprints

Filtering by Subject: Life Sciences

Reframing the habitat fragmentation debate around the inferential targets predicted by ecological theory

Juan Andrés Martínez Lanfranco, Erin M. Bayne

Published: 2026-05-14
Subjects: Life Sciences

The habitat fragmentation debate has persisted for more than three decades because dominant empirical practice has often estimated a narrower quantity than the competing ecological theories jointly require. These frameworks differ not simply in whether fragmentation matters, but in how their predicted effects change along the habitat-amount gradient. The habitat amount hypothesis (HAH) predicts [...]

The Global Biodiversity Framework supports global assessment of One Health actions

Francis Banville, Claire Burnel, Colin J Carlson, et al.

Published: 2026-05-14
Subjects: Biodiversity, Environmental Health and Protection, Environmental Monitoring, Life Sciences, Public Health

The One Health approach promotes collaboration across disciplines to enhance the health of humans, animals, plants, and the environment. Recently developed by the Quadripartite organizations, the One Health Joint Plan of Action (2022-2026) supports countries in adopting the One Health approach through six action tracks. The tracks address multiple aspects of biodiversity, containing guiding [...]

Making use of oak genomes

Andrew L Hipp, Antoine Kremer

Published: 2026-05-13
Subjects: Biodiversity, Botany, Genomics, Life Sciences

This review summarizes the contributions of genomics to our understanding of oak evolution and management, both past and ongoing. Far from being exhaustive given the large number of publications following the publication of the genomes, this review emphasizes work conducted in the decade following publication of the first two complete oak genome assemblies, and major findings and achievements [...]

HARBOUR PORPOISE RESPONSES TO PILE DRIVING ARE BETTER PREDICTED BY DISTANCE TO SOURCE THAN BY ENERGY-BASED RECEIVED SOUND LEVELS

Paul M Thompson, Aude Benhemma-Le Gall, Barbara Cheney, et al.

Published: 2026-05-13
Subjects: Life Sciences

1. Regulatory assessments for offshore construction are required to avoid impacts on protected marine mammals through noise-related injury or disturbance. Criteria for injury risk are widely accepted, but the extent to which behavioural responses are related to noise levels or co-varying contextual factors such as proximity remains uncertain. 2. This study used arrays of echolocation detectors [...]

Livestock subsidise tiger diets in a central Indian corridor: implications for human-wildlife conflict management and conservation planning

Suvankar Biswas, Sparsh Dubey, Pranav Chanchani, et al.

Published: 2026-05-12
Subjects: Life Sciences

Large carnivores, like tigers, maintain a balance within their respective ecosystem and play a critical role. However, due to their life history needs and extensive overlap with humans, they face significant threats across their distribution. These threats become severe when they inhabit non-protected areas like corridors and kill livestock. In this study, the food habits of tiger were assessed [...]

An epibiotic association between burrowing and sessile bivalves on the Amazon continental shelf: implications for ecological facilitation in sediment-dominated environments

Jonata Arruda Francisco, Flavio de Almeida Alves-Júnior, Karolina Ferreira Rodrigues, et al.

Published: 2026-05-12
Subjects: Life Sciences, Terrestrial and Aquatic Ecology

From the perspective of ecological facilitation theory, we report an epibiotic association between two mollusk species with contrasting lifestyles, Chama macerophylla Gmelin, 1791 (sessile) and Tucetona pectinata (Gmelin, 1791) (burrowing), on the Amazon continental margin. Specimens were collected using a Van Veen dredge near the shelf break and in the vicinity of AP3 blocks recently offered for [...]

Cold treatment benefits Mediterranean orchid seedlings cultivated in vitro

Gwenaëlle Deconninck, Argyrios Gerakis Gerakis, Victoria Chatzopoulou, et al.

Published: 2026-05-11
Subjects: Biology, Ecology and Evolutionary Biology, Life Sciences, Other Ecology and Evolutionary Biology, Plant Biology, Plant Sciences

1. Effective ex situ propagation is increasingly critical for the conservation and restoration of terrestrial orchids threatened by habitat loss, over-collection and climate change. However, large-scale propagation remains constrained by developmental bottlenecks during the transition from protocorms to established plantlets with storage organs. Cold exposure is often recommended to improve [...]

Hybridization in Animal Evolution

Kelsie Hunnicutt, Molly Schumer

Published: 2026-05-11
Subjects: Ecology and Evolutionary Biology, Life Sciences

In the past two decades, it has become clear that hybridization is so common in animal species as to be an almost universal feature of their evolutionary histories. Remnants of both ancient and contemporary hybridization events are present in the genomes of modern species, but their consequences are still not completely understood. In this review, we synthesize what is known about the [...]

Robustness of pesticide and other environmental stressors as key drivers of stream macroinvertebrates in small agricultural catchments

Hanh H. Nguyen, Verena C. Schreiner, Ralf B. Schäfer

Published: 2026-05-08
Subjects: Agriculture, Biodiversity, Life Sciences

Robustness of multiple stressor rankings is essential for credible ecotoxicological assessments and policy guidance. A widely cited study of 101 small agricultural streams across Germany identified pesticide mixtures as the dominant stressor for stream macroinvertebrates, but its analytical robustness has since been questioned. Using a fully reproducible workflow, we reanalysed this dataset to [...]

The social environment has little impact on inbreeding depression in a social mammal

King To Chan, Alexandre Courtiol, Leonie F. Walter, et al.

Published: 2026-05-08
Subjects: Life Sciences

Inbreeding depression describes the decline in fitness caused by breeding between relatives and is now known to be widespread in natural populations. Yet, its relative strength across different fitness components and its sensitivity to social and demographic environments are poorly understood. Using nearly 30 years of life-history, behavioural, pedigree and genomic data from a wild population of [...]

The presence of generalist plants in urban greenspaces predicts pollinator diversity

Jacob S. Francis, Nadja Pernat, Adam Bloom, et al.

Published: 2026-05-08
Subjects: Life Sciences

Urban greenspaces are increasingly important for pollinator conservation, yet it remains unclear whether pollinator diversity is better predicted by overall plant richness or by the presence of particular plant species with central roles in the pollination network, referred to as hub species. Using community science observations from 39 urban greenspaces in Broward County, Florida, we constructed [...]

From repeated baseline HRV monitoring to activity-and-recovery assessment in a trained dolphin: a preliminary protocol report

Yumi Shimozato

Published: 2026-05-08
Subjects: Life Sciences

Heart rate variability (HRV) may provide useful physiological information for welfare assessment in managed dolphins. Previous preliminary work proposed HRV analysis as a potential tool for evaluating welfare-related physiological states in dolphins. As a further step, this preliminary protocol report documents the development and initial implementation of a baseline-informed [...]

Invasiveness reshapes the historical pattern of carp trait evolution

Garen Guzmán-Rendón, Joaquín Cárcamo, Cristián E. Hernández, et al.

Published: 2026-05-07
Subjects: Ecology and Evolutionary Biology, Life Sciences

Human-mediated invasions are increasingly recognised as contemporary ecological disturbances with profound impacts on microevolutionary processes. However, whether such impacts extend beyond microevolutionary change to alter long-term evolutionary trajectories across lineages remains poorly explored. Using a global phylogenetic analysis of nearly 1,400 carp species (freshwater fishes of the [...]

Identifying knowledge gaps on simultaneous above- and belowground organism responses to global change

Hendrik Mohr, Esme Ashe-Jepson, Malte Jochum

Published: 2026-05-07
Subjects: Ecology and Evolutionary Biology, Life Sciences, Terrestrial and Aquatic Ecology

Global change affects all terrestrial organisms regardless if they live above or below the ground. Even though they are strongly linked by various direct and indirect interactions, organisms can respond very differently to global change stressors, due to different features of above- and belowground compartments. Many different organism groups have shown declines in diversity, abundance or biomass [...]

Multi-scale surveillance reveals substantial egg-laying winter activity in Aedes albopictus mosquito populations across temperate Europe

Daniele Da Re, Giovanni Marini, Alessandro Albieri, et al.

Published: 2026-05-07
Subjects: Ecology and Evolutionary Biology, Entomology, Life Sciences

The Asian tiger mosquito (Aedes albopictus), a competent vector for dengue, chikungunya, and Zika viruses, has expanded rapidly across temperate Europe. European vector surveillance typically operates May to October, assuming winter diapause precludes activity and transmission risk. However, recent field observations suggest sustained egg-laying winter activity in southern European populations, [...]

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