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Preprints

There are 2348 Preprints listed.

Avifaunal diversity in urban parks of Delhi is shaped by both anthropogenic and natural factors

Nirjesh Gautam

Published: 2025-06-13
Subjects: Ecology and Evolutionary Biology

Urbanization leads to fragmentation and reduction of natural habitats which become islands of remnant biodiversity. As predicted by the Theory of Island Biogeography (TIB), fragment area and fragment isolation are major predictors of bird species richness in urban and rural habitats. This study is to understand patterns in avifaunal composition in select urban parks specifically in terms of area [...]

Characterising the structural complexity across major habitats of Tenerife, Spain

Samantha Suter, Rüdiger Otto, José María Fernández-Palacios, et al.

Published: 2025-06-13
Subjects: Biodiversity, Ecology and Evolutionary Biology, Environmental Monitoring, Life Sciences

Understanding biodiversity changes across ecosystems requires the consideration of various biodiversity dimensions, such as habitat structural complexity – the degree of heterogeneity in the distribution of plant material in three-dimensional space. Yet, its inclusion in long-term biodiversity monitoring on oceanic islands remains limited. Terrestrial laser scanning (TLS) can be used to quantify [...]

Toxin resistance mechanisms span biological scales in the Royal Ground Snake (Colubridae: Erythrolamprus reginae)

Valeria Ramírez-Castañeda, Samantha Nixon, Dario Alarcón-Naforo, et al.

Published: 2025-06-13
Subjects: Animal Sciences, Behavior and Ethology, Biology, Cellular and Molecular Physiology, Comparative and Evolutionary Physiology, Ecology and Evolutionary Biology, Evolution, Genomics, Integrative Biology, Life Sciences, Molecular Biology, Neuroscience and Neurobiology, Physiology, Systems and Integrative Physiology Life Sciences, Systems Biology, Zoology

Exposure to multiple toxic compounds imposes diverse selective pressures, potentially leading to a toxin-resistant phenotype that operates across biological levels. There are several known toxin resistance mechanisms–such as behavioral avoidance, metabolic detoxification, and target-site insensitivity. However, most studies have been conducted with exposure to a single toxin or have focused on [...]

Genomic data confirms that mutation cannot restore genetic diversity lost through population bottlenecks

Toby Gregory Laszlo Kovacs, Simon Y. W. Ho, Carolyn J. Hogg, et al.

Published: 2025-06-13
Subjects: Ecology and Evolutionary Biology, Genomics, Life Sciences, Molecular Genetics, Population Biology

Maintaining within-species genetic diversity is a critical goal of biodiversity conservation as it determines a species’ ability to adapt to environmental change. Without human intervention, isolated populations can only recover genetic diversity post-bottleneck through the accumulation of new mutations over evolutionary timescales. Using recent estimates of mutation rates from major genomic [...]

Historical and Potential Future Importance of Marine Megafauna Subsidies to Terrestrial Ecosystems

Francis David Gerraty, Charles A Braman, Jenifer E. Dugan, et al.

Published: 2025-06-12
Subjects: Ecology and Evolutionary Biology

Marine megafauna exert tremendous influence on the structure and function of ocean ecosystems, yet mounting evidence shows that their ecological impacts also cross the land-sea interface and modify terrestrial ecosystem dynamics. Marine megafauna connect land and sea by serving as large, calorically-rich food sources for terrestrial consumers and by transferring marine-derived nutrients onto land [...]

When homology fails: lessons from liver-fluke phylogenies

Pilar Alda, Annia Alba, Nicolas Bonel

Published: 2025-06-12
Subjects: Life Sciences

Misaligned sequences derail evolutionary inference. Datasets from GenBank require verification of positional homology and orientation before alignment and phylogenetic analysis. Liver-fluke case studies reveal how overlooked errors skew results, underscoring the need for rigorous checks in parasitology and all molecular research.

Mapping disturbance across California’s rapidly changing forests

H. Anu Kramer, Elizabeth Ng, Jason Winiarski, et al.

Published: 2025-06-11
Subjects: Environmental Monitoring, Natural Resources and Conservation, Natural Resources Management and Policy, Sustainability

Disturbances shape assemblages and spatial patterns of flora and fauna across the globe, and accurate disturbance mapping can aid conservation science and decision-making. However, mapping and differentiating among disturbance types using remote sensing is challenging, especially in forests with hidden subcanopy disturbances. On federal lands in the western US, wildfire, drought, and fuels [...]

National-scale datasets systematically underestimate vegetation recovery in Australian carbon farming projects

Tim Moore, Andrew O'Reilly-Nugent, Kenneth Clarke, et al.

Published: 2025-06-10
Subjects: Environmental Monitoring

Limiting global warming below 2 degrees C requires nature-based climate solutions which are expected to supply more than a third of cost-effective climate mitigation by 2030. Regenerating native forests under the Australian Government’s Australian Carbon Credit Unit (ACCU) program are delivering large-scale carbon storage across approximately 3.4 million hectares. Projects using the Human Induced [...]

Non-native grass invasion drives biodiversity loss after a single fire in a semi-arid shrubland

Adam Lee Mahood, Jennifer Balch, David Barnard, et al.

Published: 2025-06-10
Subjects: Biodiversity, Desert Ecology, Terrestrial and Aquatic Ecology

In the United States Great Basin, invasive annual grasses have initiated a novel grass-fire cycle that has transformed vast areas of semi-arid shrublands into non-native annual grasslands that now burn frequently. After the initial transformation, the system is so fire prone that it is difficult to find areas that have not burned repeatedly. We evaluated how the ecosystem responds in the absence [...]

A draft genome assembly for the dart-poison frog Phyllobates terribilis

Roberto Marquez, Denis Jacob Machado, Reyhaneh Nouri, et al.

Published: 2025-06-10
Subjects: Biology, Genomics

Dendrobatid poison frogs have become well established as model systems in several fields of biology. Nevertheless, the development of molecular and genetic resources for these frogs has been hindered by their large, highly repetitive genomes, which have proven difficult to assemble. Here we present a draft assembly for Phyllobates terribilis (12.6Gb), generated using a combination of sequencing [...]

Marine Conservation Leadership: Does Australia Deserve a Spot on the Podium?

Carissa Klein, Kate Becker, David Carrasco Rivera, et al.

Published: 2025-06-10
Subjects: Natural Resources and Conservation

Australia’s marine biodiversity is unmatched, and its conservation has been prominently championed over the past three decades by successive Federal and State governments, who have consistently portrayed the nation as a global leader in marine protection. Here, we question whether this reputation is justified. We highlight substantial—and in some cases catastrophic—declines in marine species and [...]

Colorful birds face heightened extinction risk around the world

Montague Neate-Clegg, Natalia Ocampo-Peñuela

Published: 2025-06-10
Subjects: Biodiversity, Ecology and Evolutionary Biology, Environmental Studies, Life Sciences, Ornithology

Many of the functional traits that mediate extinction risk across the tree life relate indirectly to a species’ ability to persist in a changing world. Yet, there are certain traits such as coloration that directly affect human interactions with wildlife. Here, we use an existing dataset of color metrics for 4334 passerine bird species combined with global functional trait data to determine [...]

The interplay between ecological networks drives host-plasmid community dynamics

Ying-Jie Wang, Kaitlin Schaal, Johannes Nauta, et al.

Published: 2025-06-10
Subjects: Ecology and Evolutionary Biology, Life Sciences

Plasmids can accelerate host adaptation through horizontal gene transfer and are key members of microbial communities. Plasmid infection dynamics are potentially affected by the interplay between the structures of host-plasmid and plasmid-plasmid interactions. However, how the structures of these networks jointly affect plasmid transmission and community dynamics remains unstudied. We used an [...]

Delayed Reproduction as a Driver of Longevity

Cameron MacDonald

Published: 2025-06-09
Subjects: Life Sciences

Understanding the evolutionary forces that shape aging is central to both biology and medicine. While classic theories—such as Medawar’s mutation accumulation, Williams’ antagonistic pleiotropy, and Kirkwood’s disposable soma—have provided foundational insights, the population-level consequences of reproductive timing remain underexplored. Here, I propose that delayed reproduction may intensify [...]

Generalized graphical mixed models connect ecological theory with widely used statistical models

James T Thorson

Published: 2025-06-06
Subjects: Applied Statistics, Aquaculture and Fisheries Life Sciences, Biodiversity, Environmental Sciences, Multivariate Analysis

Ecological dynamics are analyzed across multiple sites, times, and variables. Here, we introduce the family of generalized graphical mixed models (GGMMs) and show that it extends structural equation, generalized additive, and generalized linear mixed models. GGMMs represent ecological systems using a mathematical graph, where each analytic unit (node for each site-time-variable) has a direct [...]

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